Thanks for checking out BindingTutor! Here's a quick-start tutorial designed to get you graphing binding curves in no time.
Running BindingTutor for the First Time
To begin, you will need to download and install BindingTutor. When you run BindingTutor for the first time, you should see a window that looks like this:
BindingTutor's defaults are to plot a graph of an experiment in which the total concentration of ligand, [L], is varied (along the X-axis), and the binding model is first-order binding. See the Running BindingTutor chapter for more information about these settings, and the Binding Model Mathematics chapter for more detailed information about the binding models.
First, enter values for the experimental range of [L]. Varying between 0 and 10 is a nice start.
You will also need to enter values for [P] total and KD. Try 2 for both [P] total and KD.
To calculate the binding curve under these conditions, click the graph button.
You should now see a graph that looks like this:
Congratulations, you've just plotted your first binding curve using BindingTutor!
Try changing the values of [P] total or KD to see how the curves change. After plotting four curves you'll have a graph that might look something like this:
If you'd like to start over with a new graph, click the clear button. This will delete the current graph, and subsequent curves will be plotted on a new plot.
Good luck, and happy graphing!