diff --git a/python/ipywidgets/ipywidgets/widgets/tests/test_datetime_serializers.py b/python/ipywidgets/ipywidgets/widgets/tests/test_datetime_serializers.py
index f6e6863b26..262a0aa54d 100644
--- a/python/ipywidgets/ipywidgets/widgets/tests/test_datetime_serializers.py
+++ b/python/ipywidgets/ipywidgets/widgets/tests/test_datetime_serializers.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # coding: utf-8
 
 # Copyright (c) Vidar Tonaas Fauske.
diff --git a/python/ipywidgets/ipywidgets/widgets/tests/test_widget_datetime.py b/python/ipywidgets/ipywidgets/widgets/tests/test_widget_datetime.py
index 7ebcb20a87..573d298174 100644
--- a/python/ipywidgets/ipywidgets/widgets/tests/test_widget_datetime.py
+++ b/python/ipywidgets/ipywidgets/widgets/tests/test_widget_datetime.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # coding: utf-8
 
 # Copyright (c) Jupyter Development Team.
diff --git a/python/ipywidgets/ipywidgets/widgets/tests/test_widget_naive_datetime.py b/python/ipywidgets/ipywidgets/widgets/tests/test_widget_naive_datetime.py
index 212673d5db..7e62c008cb 100644
--- a/python/ipywidgets/ipywidgets/widgets/tests/test_widget_naive_datetime.py
+++ b/python/ipywidgets/ipywidgets/widgets/tests/test_widget_naive_datetime.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # coding: utf-8
 
 # Copyright (c) Vidar Tonaas Fauske.
diff --git a/python/ipywidgets/ipywidgets/widgets/tests/test_widget_time.py b/python/ipywidgets/ipywidgets/widgets/tests/test_widget_time.py
index b9a12cb341..af9064a798 100644
--- a/python/ipywidgets/ipywidgets/widgets/tests/test_widget_time.py
+++ b/python/ipywidgets/ipywidgets/widgets/tests/test_widget_time.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 # coding: utf-8
 
 # Copyright (c) Vidar Tonaas Fauske.