Source files for a paper about identifiying sample mix-ups and mixtures in microbiome data
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Analysis
Data
R
.gitignore
LICENSE.md
Makefile
ReadMe.md
genetics.bst
mb_mixups.Rnw
mb_mixups.bib

ReadMe.md

Identification of sample mix-ups and mixtures in microbiome data in Diversity Outbred mice

Files for a paper (in preparation) on detecting sample mix-ups and mixtures in microbiome data in Diversity Outbred mice.

  • mb_mixups.Rnw - Make text for the manuscript
  • mb_mixups.bib - BibTeX file with bibliography
  • Analysis/ - directory contain basic analyses and summarized results; the raw data and pipeline to get data summaries are elsewhere
  • Data/ - summarized data sets for the analysis
  • R/ - Code for making the figures (plus the basic analyses)

The initial work involved:

  • mapping sequence reads to the mouse genome using bowtie

  • generating pileup files using tools + Rsamtools (code BamFile(), ScanBamParam(), pileup())

  • ...look for reads that overlap each possible SNP among the 8 founder lines and get counts of reads for each of the two alleles

  • separately, use calculated genotype probabilities + SNP database on founders to get inferred genotypes at all SNPs for all DO offspring

R packages needed to compile the paper:

Current PDF of the paper at https://www.biostat.wisc.edu/~kbroman/publications/mb_mixups.pdf


License

This manuscript is licensed under CC BY.

CC BY