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AZ100LightSheet

This repository contains files for building a simple light sheet system based on a Nikon AZ100 microscope. Most of the files are CAD drawings for mounting ASI LX4000 parts purchased from Ebay on to the AZ100. The filter wheel mounts were machined from aluminum by proto labs; the other parts were 3D printed from ABS in house.

What the files are

  • [AZ-FLDIC-FWup top adapter.ipt: top part of adapter for LX4000 filter wheel.](AZ-FLDIC-FWup top adapter.ipt) This bolts to the filter cube turret on the AZ100 and the filter wheel bolts to it. Machined out of aluminum. (Autocad Inventor Format)
  • [AZ-FLDIC-FWup top adapter.igs: same part, IGS format](AZ-FLDIC-FWup top adapter.igs)
  • [AZ-FLDIC-FWup bottom adapter.ipt: bottom part of adapter for LX4000 filter wheel.](AZ-FLDIC-FWup bottom adapter.ipt) This bolts to the top adapter piece and the AZ100 nosepiece bolts to it. Machined out of aluminum. (Autocad Inventor Format)
  • [AZ-FLDIC-FWup bottom adapter.igs: same part, IGS format](AZ-FLDIC-FWup bottom adapter.igs)
  • [AZ100 ASI LX-4000 stage adapter.ipt: Mount to attach LX-4000 stage to transmitted light base.](AZ100 ASI LX-4000 stage adapter.ipt) Clamps with a set screw and bolts to the stage. 3D printed from ABS. (Autocad Inventor Format)
  • [AZ100 ASI LX-4000 stage adapter.stl: same part, STL format.](AZ100 ASI LX-4000 stage adapter.stl)
  • [LX4000 Cuvette adapter.ipt: top plate for LX-4000 stage.](LX4000 Cuvette adapter.ipt) Screws to stage and accepts 8-32 press-fit threaded inserts for attaching cuvette holders with thumbscrews. (Autocad Inventor Format)
  • [LX4000 Cuvette adapter.stl: same part, STL format. (Autocad Inventor Format)](LX4000 Cuvette adapter.stl)
  • [20mm glass cuvette holder.ipt: holder for 20mm cuvettes. (Autocad Inventor Format)](20mm glass cuvette holder.ipt) Cuvettes are custom made by FireflySci
  • [20mm glass cuvette holder.stl: same part, STL format](20mm glass cuvette holder.stl)
  • [35mm glass cuvette holder.ipt: holder for 35mm cuvettes. (Autocad Inventor Format)](20mm glass cuvette holder.ipt) Cuvette is from Hellma, type 704-OG.
  • [35mm glass cuvette holder.stl: same part, STL format](35mm glass cuvette holder.stl)
  • [Cage Plate Slit 5mm.ipt: 5 mm slit assembly for mounting to Thorlabs cage system.](Cage Plate Slit 5mm.ipt) Takes press-fit threaded inserts and two razor blades are screwed to it to make a slit. (Autocad Inventor Format)
  • [Cage Plate Slit 5mm.stl: same part, STL format.](Cage Plate Slit 5mm.stl)
  • [Cage Plate Slit 10mm.ipt: 10 mm slit assembly for mounting to Thorlabs cage system.](Cage Plate Slit 10mm.ipt) Takes press-fit threaded inserts and two razor blades are screwed to it to make a slit. (Autocad Inventor Format)
  • [Cage Plate Slit 10mm.stl: same part, STL format.](Cage Plate Slit 10mm.stl)
  • [Arduino SMB-BNC box.ipt: Arduino enclosure with SMB connectors for laser launch. (Autocad Inventor Format)](Arduino SMB-BNC box.ipt)
  • [Arduino SMB-BNC box.stl: same part, STL format](Arduino SMB-BNC box.stl)
  • [Arduino SMB-BNC box lid.ipt: Lid for Arduino enclosure (Autocad Inventor Format)](Arduino SMB-BNC box lid.ipt)
  • [Arduino SMB-BNC box lid.stl: same part, STL format](Arduino SMB-BNC box lid.stl)
  • LightSheetControl: folder for LightSheetControl plugin. This works with the Utilities / MultiStage device to set the relationship between the objective and cuvette Z-stages. The user defines pairs of stage coordinates where the sample is well focused, and this plugin calculates the best fit line to the points and updates the MultiStage device appropriately.