# Download sample PacBio from the PBcR website wget -O- http://www.cbcb.umd.edu/software/PBcR/data/selfSampleData.tar.gz | tar zxf - ln -s selfSampleData/pacbio_filtered.fastq reads.fq # Install minimap and miniasm (requiring gcc and zlib) git clone https://github.com/lh3/minimap2 && (cd minimap2 && make) git clone https://github.com/lh3/miniasm && (cd miniasm && make) # Overlap for PacBio reads (or use "-x ava-ont" for nanopore read overlapping) minimap2/minimap2 -x ava-pb -t8 pb-reads.fq pb-reads.fq | gzip -1 > reads.paf.gz # Layout miniasm/miniasm -f reads.fq reads.paf.gz > reads.gfa
Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream assemblers, miniasm does not have a consensus step. It simply concatenates pieces of read sequences to generate the final unitig sequences. Thus the per-base error rate is similar to the raw input reads.
So far miniasm is in early development stage. It has only been tested on a dozen of PacBio and Oxford Nanopore (ONT) bacterial data sets. Including the mapping step, it takes about 3 minutes to assemble a bacterial genome. Under the default setting, miniasm assembles 9 out of 12 PacBio datasets and 3 out of 4 ONT datasets into a single contig. The 12 PacBio data sets are PacBio E. coli sample, ERS473430, ERS544009, ERS554120, ERS605484, ERS617393, ERS646601, ERS659581, ERS670327, ERS685285, ERS743109 and a deprecated PacBio E. coli data set. ONT data are acquired from the Loman Lab.
For a C. elegans PacBio data set (only 40X are used, not the whole dataset), miniasm finishes the assembly, including reads overlapping, in ~10 minutes with 16 CPUs. The total assembly size is 105Mb; the N50 is 1.94Mb. In comparison, the HGAP3 produces a 104Mb assembly with N50 1.61Mb. This dotter plot gives a global view of the miniasm assembly (on the X axis) and the HGAP3 assembly (on Y). They are broadly comparable. Of course, the HGAP3 consensus sequences are much more accurate. In addition, on the whole data set (assembled in ~30 min), the miniasm N50 is reduced to 1.79Mb. Miniasm still needs improvements.
Miniasm confirms that at least for high-coverage bacterial genomes, it is possible to generate long contigs from raw PacBio or ONT reads without error correction. It also shows that minimap can be used as a read overlapper, even though it is probably not as sensitive as the more sophisticated overlapers such as MHAP and DALIGNER. Coupled with long-read error correctors and consensus tools, miniasm may also be useful to produce high-quality assemblies.
Crude read selection. For each read, find the longest contiguous region covered by three good mappings. Get an approximate estimate of read coverage.
Fine read selection. Use the coverage information to find the good regions again but with more stringent thresholds. Discard contained reads.
Generate a string graph. Prune tips, drop weak overlaps and collapse short bubbles. These procedures are similar to those implemented in short-read assemblers.
Merge unambiguous overlaps to produce unitig sequences.
Consensus base quality is similar to input reads (may be fixed with a consensus tool).
Only tested on a dozen of high-coverage PacBio/ONT data sets (more testing needed).
Prone to collapse repeats or segmental duplications longer than input reads (hard to fix without error correction).