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Please, be aware that the user friendly interface is desecrated. SeqBuster only keeps updated miraligner as an independent file.

Project Status: Active – The project has reached a stable, usable state and is being actively developed.

Read how to annotate miRNAs/isomiRs from fastq files in our documentation page.

Validators of the latest versions are here.

Some comparison between many tools here.


Click here


SeqBuster is a bioinformatic tool for the processing and analysis of small RNAs datasets, reveals ubiquitous miRNA modifications in human embryonic cells. Pantano L, Estivill X, Martí E. Nucleic Acids Res. 2010 Mar;38(5):e34. Epub 2009 Dec 11.

See also

Other tools related to small RNA analysis.

  • R package isomiRs to visualize isomiRs and miRNA abundances in a group of samples (new)
  • seqcluster analyzes the rest of small RNAs and annotate to multiple databases in bed format (new)

The development of Seqbuster was partly funded by the EU Seventh Framework Programme [FP7/2007-2013, grant number 262055, ESGI].