resources for ranavirus project development
Switch branches/tags
Nothing to show
Clone or download
Fetching latest commit…
Cannot retrieve the latest commit at this time.
Permalink
Failed to load latest commit information.
Analysis
Data
Results
.DS_Store
README.md

README.md

infectious_disease_project_dev

resources for ranavirus project development

#Workflow

  1. Collecting amphibian toe/tail samples in ponds throughout NW Vermont
  2. Detecting ranavirus
  3. Analyzing

Collecting amphibian toe/tail samples in ponds throughout NW Vermont

  1. Collect every 2 weeks
  2. 18 sites

Detecting ranavirus

Notes: Samples should be stored in ethanol at -20C

Comparative methods:

Method 1: DNEasy Kit Method 2: Standard Salt Protcol

  1. Homogenize sample
  • Need beads
  1. Isolate DNA
  • Standard salt protocol is cheaper
Isolation Method Pricing per sample Total cost for 7200
Dneasy kit $2.76 $198,000
Salting method $0.30 $2,313
  1. Quantify with qcr
Species Target Primer sequence/F Primer sequence/R Annealing Temp Amplicon Length Reference
FV-3 MCP 5′-GTCTCTGGAGAAGAAGAA-3′ 5′-GAC TTGGCCACTTATGAC-3′ 52ºC --- Robert et al. 2011
FV-3 IE-ICP18 5′-ATG ATC-CAAGCCTACCTGTGC-3′ 5′-AAATGTCCTAATCTATACACC-3′ 52ºC --- Robert et al. 2011
FV-3 MCP 5′-ATGTCTTCTGTAACTGGTTCAGG-3′ 5′-GTCTCTGGAGAAGAAGAA-3′ 52ºC --- Morales and Robert 2007