This is a simple piece of software for cloning, written in Java + QT
Restriction enzyme database
- Temperatures and buffer information
- Double-digests, suggestions for best common buffer
- Simulated digests, with gel view and a range of ladders
Linear and circular view
- With smooth rotation and zooming
- Live translation to protein
Sequence property calculation
- Melting temperature calculation (Santa lucia 98)
- GC content
- Annotation on sequence
- Calculation of PCR products
- Automatically attach existing primers
- In different directions and colors
- Automatic finding of ORFs
- Automatic fitting of features
- Import from addgene
- Import/export to genbank, including ApE variant TODO: other software extensions
- Import/export to fasta
- Import from DNA Strider
- Import from SerialCloner
- Native XML format with extended metadata
- SCF files for sanger sequencing
Simulation of cloning steps
- PCR, with mutagenesis
- Annealing of oligos
- Site directed mutagenesis TODO Gibson/homology recomb/recirc after assembly
Wizards for cloning strategies
- Site direced mutagenesis
- TODO pcr/restriction
- TODO gibson
- TODO topo
- TODO TA
- TODO gateway
- Search for sequence
- Turn plasmid around
- Of course, handles restriction sites and features overlapping 0-position on circular plasmids
- Copy out sequence in various directions (reverse, complement)
- Skyline rendering of sequence
- Align sequences
- Move 0-position of plasmid
TODO: PDF export
About the sequence indices and ranges
Sequence ranges inside the code are 0-based and of the form [a,b). In other words, [0,1) selects the first base in a sequence and is of length 1. This choice is for practicality. It's simply faster and much less error prone in any modern programming language which also have arrays starting with position 0.
The user-interface can view these indices in the form of 1-index [a,b] depending on user-preference (default is to use the internal format). This is to make it easy to compare positions within the software to those displayed by other programs.
Copyright (c) 2015, Johan Henriksson, EMBL-EBI and Karolinska Institutet All rights reserved.
Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:
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