diff --git a/changelog.d/5938.misc b/changelog.d/5938.misc
new file mode 100644
index 000000000000..b5a3b6ee3b9c
--- /dev/null
+++ b/changelog.d/5938.misc
@@ -0,0 +1 @@
+Remove unused jenkins/prepare_sytest.sh file.
diff --git a/jenkins/prepare_synapse.sh b/jenkins/prepare_synapse.sh
deleted file mode 100755
index 016afb8baac0..000000000000
--- a/jenkins/prepare_synapse.sh
+++ /dev/null
@@ -1,16 +0,0 @@
-#! /bin/bash
-
-set -eux
-
-cd "`dirname $0`/.."
-
-TOX_DIR=$WORKSPACE/.tox
-
-mkdir -p $TOX_DIR
-
-if ! [ $TOX_DIR -ef .tox ]; then
-    ln -s "$TOX_DIR" .tox
-fi
-
-# set up the virtualenv
-tox -e py27 --notest -v