From 75813d1782d669b0dd115a15ccfe552bc112ae8a Mon Sep 17 00:00:00 2001 From: Maximilian Haeussler Date: Sat, 28 Dec 2019 12:41:51 +0100 Subject: [PATCH 01/16] adding long_label to pip package --- setup.py | 12 ++++++++++-- 1 file changed, 10 insertions(+), 2 deletions(-) diff --git a/setup.py b/setup.py index 60947089..eee133e4 100644 --- a/setup.py +++ b/setup.py @@ -14,8 +14,16 @@ author_email="max@soe.ucsc.edu", url="https://github.com/maximilianh/cellBrowser", description="UCSC Cellbrowser, an interactive browser for single cell data. Includes converters and basic pipelines for text files, Seurat, Scanpy and Cellranger.", - #long_description=long_description, - #long_description_content_type="text/markdown", + long_description="""The UCSC Cell Browser is an interactive browser for + single cell data, like mRNA or ATAC-seq data. You can display + dimensionality reductions, navigate them with the mouse or the cursor keys, + select cells, color by genes or meta annotations and make many other + changes. The main site runs at https://cells.ucsc.edu, but using this + package you can also convert data yourself and build a Cell Browser HTML + directory that can be served through any University webserver. You can try + the Cell Browser at https://cells.ucsc.edu or read about how to convert + data with this package on https://cellbrowser.rtfd.org.""" + long_description_content_type="text/markdown", packages=setuptools.find_packages("src/cbPyLib/"), package_dir={'':'src/cbPyLib/'}, # tell distutils packages are under src include_package_data=True, # use MANIFEST.in for non-python files From 27a381534d1c828828970d2e74fad7b445134cb2 Mon Sep 17 00:00:00 2001 From: Max Date: Sat, 28 Dec 2019 03:55:51 -0800 Subject: [PATCH 02/16] setup.py fixes --- setup.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/setup.py b/setup.py index eee133e4..7a0508f9 100644 --- a/setup.py +++ b/setup.py @@ -22,7 +22,7 @@ package you can also convert data yourself and build a Cell Browser HTML directory that can be served through any University webserver. You can try the Cell Browser at https://cells.ucsc.edu or read about how to convert - data with this package on https://cellbrowser.rtfd.org.""" + data with this package on https://cellbrowser.rtfd.org.""", long_description_content_type="text/markdown", packages=setuptools.find_packages("src/cbPyLib/"), package_dir={'':'src/cbPyLib/'}, # tell distutils packages are under src From 8afd4d6f629f07aa37ba8a80d1f8bdb59c3bf15d Mon Sep 17 00:00:00 2001 From: Matt Speir Date: Mon, 6 Jan 2020 15:18:59 -0800 Subject: [PATCH 03/16] Adding logo to Github --- docs/cellBrowserLogo.png | Bin 0 -> 38196 bytes docs/cellBrowserLogoBallOnly.png | Bin 0 -> 28581 bytes 2 files changed, 0 insertions(+), 0 deletions(-) create mode 100644 docs/cellBrowserLogo.png create mode 100644 docs/cellBrowserLogoBallOnly.png diff --git a/docs/cellBrowserLogo.png b/docs/cellBrowserLogo.png new file mode 100644 index 0000000000000000000000000000000000000000..0d05b701e7c9fd832392cf11c44e032a2ed683a6 GIT binary patch literal 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zATij|NL+5d_lHR|wfgXHfY<{n#2!Ien!y`h^G%m56ef!h-0?F{=D$xO!a^{(5^0JV zFy5^A7Sex~$H+mw(7bg8hLvd(Q8np~{0{r;y5+C=s^_-EO2Kdy=v%10T{6E$I^DJ<(|HiBR-v?;*D;7gg;FCD+d>b%+4@N;oMY>AT H^z;7$N^Dm& literal 0 HcmV?d00001 From 34a867b5c7304cd06a2c5e5b191a1f6bf466d1cd Mon Sep 17 00:00:00 2001 From: Nikolay Markov Date: Mon, 20 Apr 2020 16:48:31 -0500 Subject: [PATCH 04/16] Allow commas in cluster names in markers file --- src/cbPyLib/cellbrowser/cellbrowser.py | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/src/cbPyLib/cellbrowser/cellbrowser.py b/src/cbPyLib/cellbrowser/cellbrowser.py index 267259e1..08bad2da 100755 --- a/src/cbPyLib/cellbrowser/cellbrowser.py +++ b/src/cbPyLib/cellbrowser/cellbrowser.py @@ -17,6 +17,7 @@ from collections import namedtuple, OrderedDict from os.path import join, basename, dirname, isfile, isdir, relpath, abspath, getsize, getmtime, expanduser from time import gmtime, strftime +import csv try: # python3 @@ -2462,10 +2463,10 @@ def parseMarkerTable(filename, geneToSym): otherHeaders = headers[otherStart:otherEnd] logging.debug("Other headers: %s" % otherHeaders) + reader = csv.reader(ifh, delimiter=sep, quotechar='"') data = defaultdict(list) otherColumns = defaultdict(list) - for line in ifh: - row = line.rstrip("\r\n").split(sep) + for row in reader: clusterName = row[clusterIdx] geneId = row[geneIdx] scoreVal = float(row[scoreIdx]) From 389f4d6f6eff9e8b72d5451bc8fd83e5f59dc418 Mon Sep 17 00:00:00 2001 From: Ino de Bruijn Date: Tue, 28 Apr 2020 11:39:04 -0400 Subject: [PATCH 05/16] Fix #170 --- src/cbPyLib/cellbrowser/cellbrowser.py | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/src/cbPyLib/cellbrowser/cellbrowser.py b/src/cbPyLib/cellbrowser/cellbrowser.py index 08bad2da..0b173b20 100755 --- a/src/cbPyLib/cellbrowser/cellbrowser.py +++ b/src/cbPyLib/cellbrowser/cellbrowser.py @@ -4166,8 +4166,9 @@ def findRoot(inDir=None): def resolveOutDir(outDir): """ user can define mapping e.g. {"alpha" : "/usr/local/apache/htdocs-cells"} in ~/.cellbrowser.conf """ confDirs = getConfig("outDirs") - if outDir in confDirs: - outDir = confDirs[outDir] + if confDirs: + if outDir in confDirs: + outDir = confDirs[outDir] return outDir def build(confFnames, outDir, port=None, doDebug=False, devMode=False, redo=None): From d1852ddac810f7559b60b2e0faad97ead7402e30 Mon Sep 17 00:00:00 2001 From: Nikolay Markov Date: Thu, 21 May 2020 16:48:24 -0500 Subject: [PATCH 06/16] Fix open dataset for plain datasets, fix Esc closing for open dataset dialog --- src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js b/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js index a5bb482f..a8654b1e 100644 --- a/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js +++ b/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js @@ -932,7 +932,7 @@ var cellbrowser = function() { let doFaceting = false; let filtList = []; - if (openDsInfo.parents === undefined) { + if (openDsInfo.parents === undefined && openDsInfo.datasets !== undefined) { //noteLines.push("Filter:"); let bodyParts = getBodyParts(openDsInfo.datasets); if (bodyParts.length!==0) { @@ -1055,7 +1055,9 @@ var cellbrowser = function() { changeUrl({"ds":openDatasetName}); }); + var focused = document.activeElement; var scroller = $("#tpDatasetList").overlayScrollbars({ }); + $(focused).focus(); $("#tabLink1").tab("show"); From 04fdba61a6e077a901ffc435f47761092e99721f Mon Sep 17 00:00:00 2001 From: Nikolay Markov Date: Sun, 24 May 2020 13:58:56 -0500 Subject: [PATCH 07/16] Update legend selection checkboxes on selection actions --- src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js | 9 +++++++++ 1 file changed, 9 insertions(+) diff --git a/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js b/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js index a5bb482f..2d494714 100644 --- a/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js +++ b/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js @@ -1107,6 +1107,8 @@ var cellbrowser = function() { renderer.selectClear(); renderer.selectVisible(); renderer.drawDots(); + + $(".tpLegendCheckbox").prop("checked", true); } function onSelectNoneClick() { @@ -1114,6 +1116,8 @@ var cellbrowser = function() { clearSelectionState(); renderer.selectClear(); renderer.drawDots(); + + $(".tpLegendCheckbox").prop("checked", false); } function onSelectInvertClick() { @@ -1121,6 +1125,10 @@ var cellbrowser = function() { clearSelectionState(); renderer.selectInvert(); renderer.drawDots(); + + $(".tpLegendCheckbox").each(function() { + $(this).prop("checked", !$(this).prop("checked")); + }); } function buildOneComboboxRow(htmls, comboWidth, rowIdx, queryExpr) { @@ -5217,6 +5225,7 @@ var cellbrowser = function() { renderer.unselectByColor(valIdx); renderer.drawDots(); ev.stopPropagation(); + $(this).blur(); } function buildMinMaxPart(htmls) { From a066e9cd28d0ee154ca51460d0ef89196dcd72f6 Mon Sep 17 00:00:00 2001 From: Maximilian Haeussler Date: Mon, 25 May 2020 16:50:17 +0200 Subject: [PATCH 08/16] Update README.rst --- README.rst | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.rst b/README.rst index 9d76ee69..01803626 100644 --- a/README.rst +++ b/README.rst @@ -18,8 +18,8 @@ If you want us to add a single cell dataset to the website http://cells.ucsc.edu please contact us at cells@ucsc.edu. We are happy to add any dataset. This is a viewer for a static, precomputed layout. If you're looking for an interative layout, where you can -move the cells around and run algorithsm interactively, try Chan-Zuckerberg's own cellxgene or Spring. -Another viewer is `Scope `_. +move the cells around and run some algorithms interactively, try Chan-Zuckerberg's own cellxgene or Spring. +A website with both datasets and some analysis is `Scope `_. Apart from labs at UCSC, UCSF and Gladstone who host their data at cells.ucsc.edu, other groups have setup their own cell browsers: From ce461ce5ef08e794dad889c0560d85cae1b634f4 Mon Sep 17 00:00:00 2001 From: Maximilian Haeussler Date: Mon, 25 May 2020 17:25:03 +0200 Subject: [PATCH 09/16] Update README.rst --- README.rst | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/README.rst b/README.rst index 01803626..90b18dd1 100644 --- a/README.rst +++ b/README.rst @@ -26,14 +26,14 @@ cells.ucsc.edu, other groups have setup their own cell browsers: * Alexander Misharin Lab, Northwester University, https://www.nupulmonary.org/resources/ * Accelerating Medicine Partnership Consortium, https://immunogenomics.io/cellbrowser/, used in `Zhang et al. 2018 `_ and `Der et al 2018 `_ -* Sansom Lab, Oxford, https://sansomlab.github.io -* http://caire.ipmc.cnrs.fr/cellbrowser/Differentiation/ Zaragosi group at IPMC CNRS Nice, for the manuscript https://dev.biologists.org/content/early/2019/09/25/dev.177428.abstract +* Sansom Lab, Oxford, https://sansomlab.github.io for `Croft et al, Nature 2019 `_ +* http://caire.ipmc.cnrs.fr/cellbrowser/Differentiation/ (URL has changed, contacted authors) Zaragosi group at IPMC CNRS Nice, for the manuscript https://dev.biologists.org/content/early/2019/09/25/dev.177428.abstract Additional availability ----------------------- * The preferred installation is via pip https://pypi.org/project/cellbrowser/0.4.12/, see https://cellbrowser.readthedocs.io -* Bioconda: this tool is available to install via `bioconda `_Note that the conda release is usually a bit outdated relative to the pip release. +* Bioconda: this tool is available to install via `bioconda `_. Note that the conda release is usually a bit outdated relative to the pip release, so use pip if possible. If you cannot use pip, please contact us. * Biocontainers: there is a biocontainer automatically generated from the bioconda package available `here `_ * The Seurat3Wizard, demo at http://nasqar.abudhabi.nyu.edu/SeuratV3Wizard, builds a cell browser as its last step * Galaxy: there is a Galaxy tool for UCSC CellBrowser, which can be installed on any Galaxy instance via its `Galaxy Toolshed entry `_ or it can be directly used by users at the `Human Cell Atlas Galaxy instance `_ or as part of the example workflows, such as the `Human Cell Atlas / Scanpy CellBrowser workflow `_ or the `EBI Single Cell Expression Atlas / Scanpy / CellBrowser workflow `_ From e4b7161e9db212b1f4855b300df69386b617bed9 Mon Sep 17 00:00:00 2001 From: Maximilian Haeussler Date: Mon, 25 May 2020 17:26:20 +0200 Subject: [PATCH 10/16] Update README.rst --- README.rst | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/README.rst b/README.rst index 90b18dd1..82d26ea8 100644 --- a/README.rst +++ b/README.rst @@ -21,8 +21,7 @@ This is a viewer for a static, precomputed layout. If you're looking for an inte move the cells around and run some algorithms interactively, try Chan-Zuckerberg's own cellxgene or Spring. A website with both datasets and some analysis is `Scope `_. -Apart from labs at UCSC, UCSF and Gladstone who host their data at -cells.ucsc.edu, other groups have setup their own cell browsers: +Many labs host their data at cells.ucsc.edu by sending it to us, but some groups have setup their own cell browsers: * Alexander Misharin Lab, Northwester University, https://www.nupulmonary.org/resources/ * Accelerating Medicine Partnership Consortium, https://immunogenomics.io/cellbrowser/, used in `Zhang et al. 2018 `_ and `Der et al 2018 `_ @@ -39,8 +38,8 @@ Additional availability * Galaxy: there is a Galaxy tool for UCSC CellBrowser, which can be installed on any Galaxy instance via its `Galaxy Toolshed entry `_ or it can be directly used by users at the `Human Cell Atlas Galaxy instance `_ or as part of the example workflows, such as the `Human Cell Atlas / Scanpy CellBrowser workflow `_ or the `EBI Single Cell Expression Atlas / Scanpy / CellBrowser workflow `_ -Funded by the California Institute of Regenerative Medicine and the -Chan-Zuckerberg Initiative https://www.chanzuckerberg.com/. +This project was funded by the California Institute of Regenerative Medicine and the +Chan-Zuckerberg Initiative https://www.chanzuckerberg.com/. It is funded right now through a supplement to the NHGRI Genome Browser grant. This is early research software. You are likely to find bugs. Please open a Github ticket or email us at cells@ucsc.edu, we can usually fix them quickly. From 920ac129cbc81174d61e323e4818c5cad0aa8b0c Mon Sep 17 00:00:00 2001 From: Maximilian Haeussler Date: Mon, 25 May 2020 17:27:01 +0200 Subject: [PATCH 11/16] Update README.rst --- README.rst | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/README.rst b/README.rst index 82d26ea8..190c4231 100644 --- a/README.rst +++ b/README.rst @@ -31,15 +31,14 @@ Many labs host their data at cells.ucsc.edu by sending it to us, but some groups Additional availability ----------------------- -* The preferred installation is via pip https://pypi.org/project/cellbrowser/0.4.12/, see https://cellbrowser.readthedocs.io +* The preferred installation is via pip https://pypi.org/project/cellbrowser/, for documentation see https://cellbrowser.readthedocs.io * Bioconda: this tool is available to install via `bioconda `_. Note that the conda release is usually a bit outdated relative to the pip release, so use pip if possible. If you cannot use pip, please contact us. * Biocontainers: there is a biocontainer automatically generated from the bioconda package available `here `_ * The Seurat3Wizard, demo at http://nasqar.abudhabi.nyu.edu/SeuratV3Wizard, builds a cell browser as its last step * Galaxy: there is a Galaxy tool for UCSC CellBrowser, which can be installed on any Galaxy instance via its `Galaxy Toolshed entry `_ or it can be directly used by users at the `Human Cell Atlas Galaxy instance `_ or as part of the example workflows, such as the `Human Cell Atlas / Scanpy CellBrowser workflow `_ or the `EBI Single Cell Expression Atlas / Scanpy / CellBrowser workflow `_ - This project was funded by the California Institute of Regenerative Medicine and the -Chan-Zuckerberg Initiative https://www.chanzuckerberg.com/. It is funded right now through a supplement to the NHGRI Genome Browser grant. +Chan-Zuckerberg Initiative https://www.chanzuckerberg.com/. In 2020, it is funded through a supplement to the NHGRI Genome Browser grant. This is early research software. You are likely to find bugs. Please open a Github ticket or email us at cells@ucsc.edu, we can usually fix them quickly. From 268751654bc5378b4fc5035b5568014656145375 Mon Sep 17 00:00:00 2001 From: Nikolay Markov Date: Mon, 1 Jun 2020 18:08:16 -0500 Subject: [PATCH 12/16] Change selection to Set, toggle legend checkboxes based on selection --- .../cellbrowser/cbWeb/js/cellBrowser.js | 70 ++++--------- src/cbPyLib/cellbrowser/cbWeb/js/maxPlot.js | 97 +++++++++---------- 2 files changed, 65 insertions(+), 102 deletions(-) diff --git a/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js b/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js index 2d494714..1c560438 100644 --- a/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js +++ b/src/cbPyLib/cellbrowser/cbWeb/js/cellBrowser.js @@ -1074,8 +1074,11 @@ var cellbrowser = function() { } - function onSelChange(cellIds) { + function onSelChange(selection) { /* called each time when the selection has been changed */ + var cellIds = []; + selection.forEach(function(x) {cellIds.push(x)}); + if (cellIds.length===0 || cellIds===null) { clearMetaAndGene(); clearSelectionState(); @@ -1093,6 +1096,21 @@ var cellbrowser = function() { if ("geneSym" in gLegend) buildViolinPlot(); + var cols = renderer.col.arr; + var selectedLegends = {}; + for (var i = 0; i < gLegend.rows.length; i++) { + selectedLegends[i] = 0; + } + selection.forEach(function(cellId) { + selectedLegends[cols[cellId]]++; + }); + for (var i = 0; i < gLegend.rows.length; i++) { + if (selectedLegends[i] == gLegend.rows[i].count) { + $("#tpLegendCheckbox_" + i).prop("checked", true); + } else { + $("#tpLegendCheckbox_" + i).prop("checked", false); + } + } } function onSaveAsClick() { @@ -1107,8 +1125,6 @@ var cellbrowser = function() { renderer.selectClear(); renderer.selectVisible(); renderer.drawDots(); - - $(".tpLegendCheckbox").prop("checked", true); } function onSelectNoneClick() { @@ -1116,8 +1132,6 @@ var cellbrowser = function() { clearSelectionState(); renderer.selectClear(); renderer.drawDots(); - - $(".tpLegendCheckbox").prop("checked", false); } function onSelectInvertClick() { @@ -1125,10 +1139,6 @@ var cellbrowser = function() { clearSelectionState(); renderer.selectInvert(); renderer.drawDots(); - - $(".tpLegendCheckbox").each(function() { - $(this).prop("checked", !$(this).prop("checked")); - }); } function buildOneComboboxRow(htmls, comboWidth, rowIdx, queryExpr) { @@ -5088,49 +5098,9 @@ var cellbrowser = function() { function onLegendLabelClick(ev) { /* called when user clicks on legend entry. */ - //function saveLabel() { - ///* save the current labelEl text to the cart and update everything */ - //$(".tooltip").remove(); // not sure why tooltips won't disappear here - //labelEl.removeAttr("contenteditable"); - //var newLabel = labelEl.text(); // = strip the rich text tags possibly added through copy/paste - //var metaInfo = gLegend.metaInfo; - //cartFieldArrayUpdate(db, metaInfo, "shortLabels", legendId, newLabel); - //legendUpdateLabels(gLegend.metaInfo.name); - //rendererUpdateLabels(metaInfo); - //buildLegendBar(); - //renderer.drawDots(); - //} - var legendId = parseInt(ev.target.id.split("_")[1]); var colorIndex = gLegend.rows[legendId].intKey; - - //if (("lastClicked" in gLegend) && gLegend.lastClicked===legendId) { - // user clicked the same entry as before: - //gLegend.lastClicked = null; - //$('#tpLegend_'+legendId).removeClass('tpLegendSelect'); - //renderer.selectClear(); - //} - //else { - // clear the old selection - //if (!ev.shiftKey && !ev.ctrlKey && !ev.metaKey) { - //renderer.selectClear(); - //$('.tpLegend').removeClass('tpLegendSelect'); - //} - $("#tpLegendCheckbox_"+colorIndex).prop("checked", true); - renderer.selectByColor(colorIndex); - //menuBarShow("#tpFilterButton"); - //menuBarShow("#tpOnlySelectedButton"); - //$('#tpLegend_'+legendId).addClass('tpLegendSelect'); - //gLegend.lastClicked=legendId; - clearSelectionState(); - //if (gLegend.type==="meta" && gLegend.metaInfo.type==="enum") { - //let fieldName = gLegend.metaInfo.name; - //let fieldVal = gLegend.metaInfo.valCounts[colorIndex][0]; - //let queryList = [{"m":fieldName, "eq":fieldVal}]; - //saveQueryList(queryList); - //} - //} - renderer.drawDots(); + $("#tpLegendCheckbox_" + colorIndex).click(); } function onSortByClick (ev) { diff --git a/src/cbPyLib/cellbrowser/cbWeb/js/maxPlot.js b/src/cbPyLib/cellbrowser/cbWeb/js/maxPlot.js index b84a04db..7bfe91e6 100644 --- a/src/cbPyLib/cellbrowser/cbWeb/js/maxPlot.js +++ b/src/cbPyLib/cellbrowser/cbWeb/js/maxPlot.js @@ -174,7 +174,7 @@ function MaxPlot(div, top, left, width, height, args) { self.col.pal = null; // list of six-digit hex codes self.col.arr = null; // length is coords.px/2, one byte per cell = index into self.col.pal - self.selCells = []; // IDs of cells that are selected (drawn in black) + self.selCells = new Set(); // IDs of cells that are selected (drawn in black) self.doDrawLabels = true; // should cluster labels be drawn? self.initPort(args); @@ -652,13 +652,12 @@ function MaxPlot(div, top, left, width, height, args) { // draw the selection as black rectangles ctx.globalAlpha = 0.7; ctx.fillStyle="black"; - for (i = 0; i < selCells.length; i++) { - var cellId = selCells[i]; + selCells.forEach(function(cellId) { let pxX = pxCoords[2*cellId]; let pxY = pxCoords[2*cellId+1]; ctx.fillRect(pxX-radius, pxY-radius, dblSize, dblSize); count += 1; - } + }) console.log(count+" rectangles drawn (including selection)"); ctx.restore(); return count; @@ -907,18 +906,17 @@ function MaxPlot(div, top, left, width, height, args) { // overdraw the selection as solid black circle outlines ctx.globalAlpha = 0.7; - for (i = 0; i < selCells.length; i++) { - var cellId = selCells[i]; + selCells.forEach(function(cellId) { let pxX = pxCoords[2*cellId]; let pxY = pxCoords[2*cellId+1]; if (isHidden(pxX, pxY)) - continue; + return; // make sure that old leftover overlapping black circles don't shine through let col = coordColors[cellId]; ctx.drawImage(off, col * tileWidth, 0, tileWidth, tileHeight, pxX - radius -1, pxY - radius-1, tileWidth, tileHeight); ctx.drawImage(off, selImgId * tileWidth, 0, tileWidth, tileHeight, pxX - radius -1, pxY - radius-1, tileWidth, tileHeight); - } + }) console.log(count +" circles drawn"); ctx.restore(); @@ -1020,17 +1018,16 @@ function MaxPlot(div, top, left, width, height, args) { } // overdraw the selection as black pixels - for (i = 0; i < selCells.length; i++) { - var cellId = selCells[i]; + selCells.forEach(function(cellId) { let pxX = pxCoords[2*cellId]; let pxY = pxCoords[2*cellId+1]; if (isHidden(pxX, pxY)) - continue; + return; let p = 4 * (pxY*width+pxX); // pointer to red value of pixel at x,y cData[p] = 0; cData[p+1] = 0; cData[p+2] = 0; - } + }) self.ctx.putImageData(canvasData, 0, 0); return count; @@ -1493,30 +1490,29 @@ function MaxPlot(div, top, left, width, height, args) { this.selectClear = function() { /* clear selection */ - self.selCells.length = 0; + self.selCells.clear(); setStatus(""); if (self.onSelChange!==null) - self.onSelChange([]); + self.onSelChange(self.selCells); }; this.selectSet = function(cellIds) { /* set selection to an array of integer cellIds */ - self.selCells.length = 0; + self.selCells.clear(); //self.selCells.push(...cellIds); // "extend" = array spread syntax, https://davidwalsh.name/spread-operator let selCells = self.selCells; for (let i=0; i

  • Rename clusters...
    • '); htmls.push('
  • Remove all custom annotations
    • '); //htmls.push('
  • Run clustering...
    • '); + htmls.push('
  • Set as background cellsb s
    • '); + htmls.push('
  • Reset background cellsb r
    • '); htmls.push(''); // Tools dropdown-menu htmls.push(''); // Tools dropdown container @@ -2224,6 +2250,8 @@ var cellbrowser = function() { $('#tpRenameClusters').click( onRenameClustersClick ); $('#tpCustomAnnots').click( onCustomAnnotationsClick ); + $('#tpSetBackground').click( onBackgroudSetClick ); + $('#tpResetBackground').click( onBackgroudResetClick ); //$('#tpCluster').click( onRunClusteringClick ); // This version is more like OSX/Windows: @@ -2501,10 +2529,13 @@ var cellbrowser = function() { var labelLines = []; //labelLines.push([labelList[0], dataList[0].length+" cells"]); - labelLines.push([labelList[0], dataList[0].length]); - if (dataList.length > 1) + labelLines[0] = labelList[0].split("\n"); + labelLines[0].push(dataList[0].length); + if (dataList.length > 1) { //labelLines.push([labelList[1], dataList[1].length+" cells"]); - labelLines.push([labelList[1], dataList[1].length]); + labelLines[1] = labelList[1].split("\n"); + labelLines[1].push(dataList[1].length); + } const ctx = document.getElementById("tpViolinCanvas").getContext("2d"); @@ -2587,6 +2618,31 @@ var cellbrowser = function() { var dataList = []; var labelList = []; var selCells = renderer.getSelection(); + + // filter exprVec by background + if (background !== null) { + var ourSelCells = {}; + for (var i = 0; i < selCells.length; i++) { + ourSelCells[selCells[i]] = true; + } + var ourCells = {}; + for (i = 0; i < background.length; i++) { + ourCells[background[i]] = true; + } + + var result = []; + var renamedSelCells = []; + for (i = 0; i < exprVec.length; i++) { + if (i in ourSelCells) { + renamedSelCells.push(result.length); + result.push(exprVec[i]); + } else if (i in ourCells) { + result.push(exprVec[i]); + } + } + exprVec = result; + selCells = renamedSelCells; + } // if we have a violin meta field to split on, make two violin plots, one meta vs, the other meta // restrict the plot to the selected cells, if any if (db.conf.violinField!==undefined) { @@ -2596,12 +2652,20 @@ var cellbrowser = function() { if (selCells.length===0) { // no selection, no violinField: default to a single violin plot dataList = [Array.prototype.slice.call(exprVec)]; - labelList = ['All cells']; + if (background === null) { + labelList = ['All cells']; + } else { + labelList = ['Background\ncells']; + } buildViolinFromValues(labelList, dataList); } else { // cells are selected and no violin field: make two violin plots, selected against other cells dataList = splitExpr(exprVec, selCells); - labelList = ['Selected', 'Others']; + if (background === null) { + labelList = ['Selected', 'Others']; + } else { + labelList = ['Selected', 'Background\nOthers']; + } if (dataList[1].length===0) { dataList = [dataList[0]]; labelList = ['All Selected']; @@ -4888,6 +4952,8 @@ var cellbrowser = function() { Mousetrap.bind('s i', onSelectInvertClick); Mousetrap.bind('s s', onSelectNameClick); + Mousetrap.bind('b s', onBackgroudSetClick); + Mousetrap.bind('b r', onBackgroudResetClick); Mousetrap.bind('m', function() {$('#tpMetaCombo').trigger("chosen:open"); return false;}); Mousetrap.bind('d', function() {$('#tpDatasetCombo').trigger("chosen:open"); return false;});