CRISPOR - a CRISPR/Cas9 assistant
CRISPOR predicts off-targets in the genome, ranks guides, highlights problematic guides, designs primers and helps with cloning. Try it on http://crispor.org
CRISPOR uses BWA, a few tools from the UCSC Genome Browser (twoBitToFa, bedClip), various R packages and a huge collection of external packages and source code files from published articles, see the file crisporEffScores.py for the exact references.
If you only need efficiency scores and no interactive website, try "python crisporEffScores.py", it is a python module but also has a command line interface that may be sufficient for programmers.
Installation of CRISPOR
Install BWA and a few required python modules:
# Debian/Ubuntu apt-get install bwa python-pip python-matplotlib sudo pip install biopython numpy scikit-learn==0.16.1 pandas twobitreader
# Fedora/Centos/Redhat/Scientific Linux yum install bwa python-pip python-devel tkinter sudo pip install biopython numpy scikit-learn==0.16.1 pandas matplotlib twobitreader
For the Cpf1 scoring model:
sudo pip install keras tensorflow h5py
Install required R libraries:
sudo Rscript -e 'install.packages(c("e1071"), repos="http://cran.rstudio.com/")' sudo Rscript -e 'source("https://bioconductor.org/biocLite.R"); biocLite(c("limma"));'
When you run crispor.py, it should show the usage message:
Usage: crispor.py [options] org fastaInFile guideOutFile Command line interface for the Crispor tool. org = genome identifier, like hg19 or ensHumSap fastaInFile = Fasta file guideOutFile = tab-sep file, one row per guide Use "noGenome" if you only want efficiency scoring (a LOT faster). This option will use BWA only to match the sequence to the genome, extend it and obtain efficiency scores. If many guides have to be scored in batch: Add GGG to them to make them valid guides, separate these sequences by at least one "N" character and supply as a single fasta sequence, a few dozen to ~100 per file. Options: -h, --help show this help message and exit -d, --debug show debug messages, do not delete temp directory -t, --test run internal tests -p PAM, --pam=PAM PAM-motif to use, default NGG. TTTN triggers special Cpf1 behavior: no scores anymore + the PAM is assumed to be 5' of the guide. Common PAMs are: NGG,TTTN,NGA,NGCG,NNAGAA,NGGNG,NNGRRT,NNNNGMTT,NNNNACA -o OFFTARGETFNAME, --offtargets=OFFTARGETFNAME write offtarget info to this filename -m MAXOCC, --maxOcc=MAXOCC MAXOCC parameter, guides with more matches are excluded --mm=MISMATCHES maximum number of mismatches, default 4 --bowtie new: use bowtie as the aligner. Do not use. Bowtie misses many off-targets. --skipAlign do not align the input sequence. The on-target will be a random match with 0 mismatches. --noEffScores do not calculate the efficiency scores --minAltPamScore=MINALTPAMSCORE minimum MIT off-target score for alternative PAMs, default 1.0 --worker Run as worker process: watches job queue and runs jobs --clear clear the worker job table and exit -g GENOMEDIR, --genomeDir=GENOMEDIR directory with genomes, default ./genomes
Testing the script
To test the program, first make sure that there is a directory "../genomes". If it's not there, rename "genomes.sample" to "genomes":
mv ../genomes.sample ../genomes
Then run this command:
mkdir -p sampleFiles/mine/ crispor.py sacCer3 sampleFiles/in/sample.sacCer3.fa sampleFiles/mine/sample.sacCer3.tsv -o sampleFiles/mine/sample.sacCer3.mine.offs.tsv
The files in sampleFiles/mine should be identical to the files in sampleFiles/out/
The file testInHg19.fa contains a sample for the hg19 genome, the output is in testOutHg19.tab and testOutHg19Offtargets.tab
../crispor.py hg19 testInHg19.fa testOutHg19.mine.tab -o testOutHg19Offtargets.mine.tab
Running the script as a CGI under Apache with the job queue
Make sure you can execute CGI scripts somewhere. Your Apache config (e.g. /etc/apache2/sites-enabled/000-default) should contain a section like this:
<Directory "/var/www/html"> AllowOverride All Options +ExecCGI (...) AddHandler cgi-script .cgi .pl .py
Also make sure you have the CGI module enabled:
sudo a2enmod cgi sudo service apache2 restart
If using SElinux, especially on Fedora/CentOS/RedHat, please switch it off or set it to permissive mode.
Clone the repo into such a directory:
cd /var/www/html/ git clone https://github.com/maximilianh/crisporWebsite
Use the sample E. coli genome for a start:
mv genomes.sample genomes
Create a temp directory with the right permissions:
mkdir temp chmod a+rw temp
Make sure that Apache is allowed to execute the crispor.py script, it should have x and r permissions for all:
ls -la crispor.py # if not ... chmod a+rx crispor.py
By default, the jobs database is a SQlite file, /tmp/crisporJobs.db. The Apache user has to be able to write to it so let us create it now:
./crispor.py --clear Worker queue now empty
Now start a single worker job. It will watch the job queue and process jobs:
Check that your worker is indeed running:
cat log/worker1.log ps aux | grep crispor
Now try to access the script from a webbrowser, http://localhost/crispor.py and click "Submit"
Adding a genome
Look into the "tools" directory [https://github.com/maximilianh/crisporWebsite/tree/master/tools], try the script crisprAddGenome.
The subdirectory usrLocalBin contains required tools for this script, you can copy them into /usr/local/bin of your machine, they are 64bit static linux binaries and should work on most current machines.
The script can auto-download genomes from Ensembl, UCSC or NCBI or allows you to add your own custom genome in .fasta format and .gff.
E.g. to add the X. laevis genome: sudo crisprAddGenome fasta /tmp2/LAEVIS_7.1.repeatMasked.fa --desc 'xenBaseLaevis71|Xenopus laevis|X. laevis|Xenbase V7.1' --gff geneModels.gff3
The four |-split values for the --desc option are: internalDatabaseName, scientificName, commonOrDisplayName, VersionNameOfAssembly
Make sure that internalDatabaseName does not include special characters, spaces etc. as it is used for the directory name.
- Jean-Paul Concordet for numerous ideas on the user interface
- Alberto Stolfi for the finding the N-SNP-bug
- Mark Diekhans for patching twoBitToFa and making it 100 times faster
- See the file changes.html for the full list of acknowledgements for every feature
- BWA is under GPL3
- libSVM: under copyright by Chih-Chung Chang and Chih-Jen Lin see http://www.csie.ntu.edu.tw/~cjlin/libsvm/COPYRIGHT
- svmlight: free for non-commercial use, see http://svmlight.joachims.org/
- SSC: no license specified
- primer3: GPL2.
- Fusi/Doench score: see LICENSE.txt, (c) by Microsoft Research
- the two files crispor.py and crisporEffScores.py are released under a special license, see LICENSE.txt in this directory