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| -Real-time conversion and analysis of Nanopore reads | ||
| +npReader is a tool for real-time conversion and analysis of Nanopore | ||
| +reads. It is included in the Japsa package, and its documentation is | ||
| +on http://japsa.readthedocs.org/en/latest/tools/jsa.np.f5reader.html | ||
| -I. Windows | ||
| - | ||
| -1. Installation: | ||
| - - Download and install HDF-Java (https://www.hdfgroup.org/products/java/release/download.html) | ||
| - | ||
| - - Download either japsa*.tar.gz or japsa*.zip and extract to a directory, e.g., c:\JapsaHome. (So you will see c:\JapsaHome\lib\japsa\japsa.jar) | ||
| - | ||
| - - Set up the following environment variables: | ||
| - a. JSA_HOME to the directory where japsa was installed (e.g., c:\JapsaHome) | ||
| - b. JHDF5_LIB to the library directory of HDF-Java that contains files jhdf5.dll and jhdf.dll, e.g., C:\Program Files\HDF_Group\HDFView\2.11.0\lib | ||
| - | ||
| -2. Running | ||
| - Run jsa.np.f5readerGUI.bat from either command line or mouse click. On the GUI, enter the directory that the Metricho downloads to, and select output file. | ||
| - | ||
| - | ||
| - | ||
| - | ||
| -II. Linux | ||
| - | ||
| -1. Installation | ||
| - - Make sure libjhdf5 was installed | ||
| - - check out japsa | ||
| -$git clone https://github.com/mdcao/japsa | ||
| - | ||
| -- Compile and install | ||
| -$cd japsa | ||
| -$make -f Makefile.main INSTALL_DIR=<INTALL_DIRECTORY> JLP=<PATH_TO_LIBJDH5.SO> | ||
| - | ||
| -2. Run | ||
| -$jsa.np.f5reader --folder <PATH_TO_DOWLOAD> -output <outputfile.fastq> -GUI | ||
| - | ||
| - | ||
| - You can even pass the fastq to a downstream analysis program, such as bwa: | ||
| - | ||
| - $jsa.np.f5reader --folder <PATH_TO_DOWLOAD> -output - -GUI | tee output.fastq | bwa mem <path_to_index> - > output.sam | ||
| - | ||
| - | ||
| - |
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