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| +Real-time conversion and analysis of Nanopore reads | ||
| + | ||
| +I. Windows | ||
| + | ||
| +1. Installation: | ||
| + - Download and install HDF-Java (https://www.hdfgroup.org/products/java/release/download.html) | ||
| + | ||
| + - Download either japsa*.tar.gz or japsa*.zip and extract to a directory, e.g., c:\japsa | ||
| + | ||
| + - Set up the following environment variables: | ||
| + a. JSA_HOME to the directory where japsa was installed (e.g., c:\japsa) | ||
| + b. JHDF5_LIB to the library directory of HDF-Java that contains files jhdf5.dll and jhdf.dll, e.g., C:\Program Files\HDF_Group\HDFView\2.11.0\lib | ||
| + | ||
| +2. Running | ||
| + Run jsa.np.f5readerGUI.bat from either command line or mouse click. On the GUI, enter the directory that the Metricho downloads to, and select output file. | ||
| + | ||
| + | ||
| + | ||
| + | ||
| +II. Linux | ||
| + | ||
| +1. Installation | ||
| + - Make sure libjhdf5 was installed | ||
| + - check out japsa | ||
| +$git clone https://github.com/mdcao/japsa | ||
| + | ||
| +- Compile and install | ||
| +$cd japsa | ||
| +$make -f Makefile.main INSTALL_DIR=<INTALL_DIRECTORY> JLP=<PATH_TO_LIBJDH5.SO> | ||
| + | ||
| +2. Run | ||
| +$jsa.np.f5reader --folder <PATH_TO_DOWLOAD> -output <outputfile.fastq> -GUI | ||
| + | ||
| + | ||
| + | ||
| + | ||
| + |
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