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| +### Introduction | ||
| + | ||
| +npReader is a tool to facilitate real-time conversion and analysis of Nanopore sequencing | ||
| +data. Its source code is part of [Japsa](https://github.com/mdcao/japsa) package. | ||
| + | ||
| +### Quick installation guide | ||
| + | ||
| +######Under Linux/Mac | ||
| + | ||
| +1.Install JHI5 using the packaging tool of your operating system distribution. For example, under Debian/Ubuntu | ||
| + | ||
| + sudo apt-get install libjhdf5-jni | ||
| + | ||
| +2.Obtain the source code, compile and install | ||
| + | ||
| + git clone https://github.com/mdcao/japsa | ||
| + cd japsa | ||
| + make install JLP=/usr/lib/jni \ | ||
| + [INSTALL_DIR=~/.usr/local \] | ||
| + [MXMEM=7000m \] | ||
| + [SERVER=true \] | ||
| + | ||
| +Change the JLP directive to point to where JHI5 is installed if needed. | ||
| + | ||
| +3.Set the environment variable PATH to include the installation directory, eg, | ||
| + | ||
| + set PATH=~/.usr/local/bin:$PATH | ||
| + | ||
| +4.Usage information | ||
| + | ||
| + jsa.np.f5reader --help | ||
| + | ||
| +######On any OSs | ||
| + | ||
| +1.Install JNI from https://www.hdfgroup.org/products/java/JNI/jhi5/index.html | ||
| + | ||
| +2.Download pre-compiled Japsa of the latest release from https://github.com/mdcao/japsa/releases | ||
| + | ||
| +3.Unzip the package, and run install.sh (for Linux/Mac) or install.bat (for Windows) and follow the prompt. | ||
| + | ||
| + | ||
| +### Documentation | ||
| + | ||
| +Detailed usage of npReader including setting up streaming analysis of nanopore sequencing in | ||
| +genuain real-time can be found from http://japsa.readthedocs.org/en/latest/tools/jsa.np.f5reader.html |
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