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update readme

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1 parent edf34eb commit f3aef35cdaf6f5c50c8d2ccdf56e76f852fefefc @mdcao committed Jun 25, 2015
Showing with 6 additions and 3 deletions.
  1. +6 −3 README
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9 README
@@ -5,10 +5,10 @@ I. Windows
1. Installation:
- Download and install HDF-Java (https://www.hdfgroup.org/products/java/release/download.html)
- - Download either japsa*.tar.gz or japsa*.zip and extract to a directory, e.g., c:\japsa
+ - Download either japsa*.tar.gz or japsa*.zip and extract to a directory, e.g., c:\JapsaHome. (So you will see c:\JapsaHome\lib\japsa\japsa.jar)
- Set up the following environment variables:
- a. JSA_HOME to the directory where japsa was installed (e.g., c:\japsa)
+ a. JSA_HOME to the directory where japsa was installed (e.g., c:\JapsaHome)
b. JHDF5_LIB to the library directory of HDF-Java that contains files jhdf5.dll and jhdf.dll, e.g., C:\Program Files\HDF_Group\HDFView\2.11.0\lib
2. Running
@@ -32,6 +32,9 @@ $make -f Makefile.main INSTALL_DIR=<INTALL_DIRECTORY> JLP=<PATH_TO_LIBJDH5.SO>
$jsa.np.f5reader --folder <PATH_TO_DOWLOAD> -output <outputfile.fastq> -GUI
-
+ You can even pass the fastq to a downstream analysis program, such as bwa:
+
+ $jsa.np.f5reader --folder <PATH_TO_DOWLOAD> -output - -GUI | tee output.fastq | bwa mem <path_to_index> - > output.sam
+
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