diff --git a/docs/assembly_qc/assembly.rst b/docs/assembly_qc/assembly.rst index 3517b58..9193ce8 100644 --- a/docs/assembly_qc/assembly.rst +++ b/docs/assembly_qc/assembly.rst @@ -76,15 +76,15 @@ We will run the assembly in two steps. Generate corrected reads ------------------------ -Command:: +The correction stage selects the best overlaps to use for correction, estimates corrected read lengths, and generates corrected reads:: - canu -correct -d ~/canu_correct -p 'canuAssembly' 'genomeSize=3m' 'gnuPlotTested=true' 'useGrid=false' 'maxThreads=16' 'minThreads=16' -nanopore-raw D1_basecall/workspace/pass/ + canu -correct -d ~/canu_correct -p 'canuAssembly' 'genomeSize=3m' 'gnuPlotTested=true' 'useGrid=false' -nanopore-raw 1D_basecall.fastq Genereate and assemble trimmed reads ----------------------- -Command:: +The trimming stage identifies unsupported regions in the input and trims or splits reads to their longest supported range. The assembly stage makes a final pass to identify sequencing errors; constructs the best overlap graph (BOG); and outputs contigs, an assembly graph, and summary statistics:: canu -trim-assemble -d ~/canu_assembly -p 'canuAssembly' 'genomeSize=3M' 'useGrid=false' 'minThreads=16' 'maxThreads=16' 'gnuPlotTested=true' -nanopore-corrected ~/canu_correct/canuAssembly.correctedReads.fasta.gz -nanopore-corrected ~/D1_2_basecall/workspace/fastq_runid_*