diff --git a/_pages/Device_Support.md b/_pages/Device_Support.md index a780d154..cfbad7b3 100644 --- a/_pages/Device_Support.md +++ b/_pages/Device_Support.md @@ -386,6 +386,7 @@ release](Download_Micro-Manager_Latest_Release). - [Oxxius](Oxxius) - Oxxius Laser Controller - [Oxxius\_combiner](Oxxius_combiner) - Oxxius combiner; hub for multiple laser sources +- [RappLasers](RappLasers) - Rapp laser controllers - [PolychromeV](PolychromeV) - Illumination system from Till Photonics - [Prairie Aurora Laser @@ -454,8 +455,10 @@ release](Download_Micro-Manager_Latest_Release). Stabilization from [BIG](http://big.umassmed.edu) - [PriorPureFocus](PriorPureFocus) - Hardware autofocus device from Prior - [PyDevice](PyDevice) (Experimental) - Devices controlled by Python scripts -- [Rapp](Rapp) - Rapp UGA40 photobleaching/photoactivation +- [Rapp](Rapp) - Rapp UGA-40 photobleaching/photoactivation unit +- [Rapp_UGA42](Rapp_UGA42) - Rapp UGA-42 galvo scanner for + phototargeting - [SimpleAutofocus](SimpleAutofocus) - Image-based autofocus software 'device'. - [SpectralLMM5](SpectralLMM5) - Spectral laser line diff --git a/_pages/RappLasers.md b/_pages/RappLasers.md new file mode 100644 index 00000000..8ca6b338 --- /dev/null +++ b/_pages/RappLasers.md @@ -0,0 +1,55 @@ +--- +autogenerated: false +title: RappLasers +redirect_from: /wiki/RappLasers +layout: page +--- + +| | | +|------------|--------------------------------------| +| Summary: | Rapp Laser Controller | +| Author: | Nico Stuurman | +| License: | BSD | +| Platforms: | Windows | +| Devices: | Rapp laser controllers | + +------------------------------------------------------------------------ + +The RappLasers device adapter controls Rapp laser controllers, providing +shutter control, light output control, and intensity adjustment for Rapp +laser systems. For convencience, the laser are implemented as shutters. The shutter will only work in Software control mode and will control the "Light" (laser on/off) rather than the "Shutter". + +## Properties + +| Property | Description | +|---------------|--------------------------------------------------------------------------------------------------| +| Port | Serial COM port for communication with the laser controller | +| Shutter | Controls the hardware shutter (Open/Closed). This is more of a safety shutter that is not meant to be switched fast and often. | +| Light | Controls the software laser output (On/Off) | +| Intensity (%) | Sets the laser power from 0-100% | +| ControlMode | Selects the control mode: Software, External-Mix, External-Digital, RMI-Mix, RMI-Digital, External-Analog, or RMI-Analog | +| SerialNumber | Read-only device serial number | +| LaserName | Read-only device model identifier | + +## Control Modes + +The laser supports multiple control modes: + +- **Software** (default): Laser is controlled entirely through software commands +- **External-Mix**: Mixed external control +- **External-Digital**: Digital external triggering +- **RMI-Mix**: Mixed RMI control +- **RMI-Digital**: Digital RMI control +- **External-Analog**: Analog external control +- **RMI-Analog**: Analog RMI control + +External refers to BNC connectors on the controller, RMI to another type of connector (first time I have heard of these, and I can not find them with a few searches, so maybe something Rapp specific). + +## Installation + +Configure the laser controller in the Hardware Configuration Wizard by +selecting "RappLasers" from the device list and specifying the appropriate +COM port. Standard (default) serial port settings will work. + +## Normal Usage +Most often, these lasers will be used in conjunction with the Rapp UGA-42 device for photo-bleaching/photo-activation. In that case, make sure to open the shutter, and set the ControMode to RMI-Mix. This is most conveniently done in the System-Startup configuration. Please note that in RMI-Mix (or External-Mix) modes the Light and Intensity controls will not work (they are replaces by the external inputs). diff --git a/_pages/Rapp_UGA42.md b/_pages/Rapp_UGA42.md new file mode 100644 index 00000000..c499e653 --- /dev/null +++ b/_pages/Rapp_UGA42.md @@ -0,0 +1,65 @@ +--- +autogenerated: false +title: Rapp_UGA42 +redirect_from: /wiki/Rapp_UGA42 +layout: page +--- + +| | | +|------------|--------------------------------------| +| Summary: | Rapp UGA-42 Galvo Scanner | +| Author: | Nico Stuurman | +| License: | BSD | +| Platforms: | Windows | +| Devices: | Rapp UGA-42 | + +------------------------------------------------------------------------ + +The Rapp UGA-42 is a galvanometer-based laser targeting system for +photobleaching, photoactivation, and other phototargeting applications. +It uses galvo mirrors to precisely position laser beams through the +microscope objective to target specific locations in the specimen. + +This device adapter is similar to the original [Rapp](Rapp) adapter for +the UGA-40, but is designed for the newer UGA-42 hardware. + +The Micro-Manager [Projector Plugin](Projector_Plugin) allows microscope +operators to calibrate the UGA42 with the camera and to draw spots or +regions of interest on an image and phototarget these areas +using the UGA-42. + +## Features + +- Multi-laser support (Laser 1 through 4 +- Point-and-fire capability for single-spot targeting +- Polygon support for complex ROI targeting +- Sequence automation for programmed scanning patterns +- TTL triggering with configurable edge detection + +## Properties + +| Property | Description | +|---------------------|-------------------------------------------------------------------------------------------------| +| LaserPor | Selects which laser to use (Laser 1 - 4) | +| LaserIntensity | Controls analog voltage send to the laser, hence laser intensity | +| LaserType | Continuous or pulsed | +| MaxIntensity | Max Laser Intensity | +| MinIntensity | Minimum Laser Intensity | +| SpotSize | Laser spot diameter for ROI rastering calculations, relative number of unknown dimensions | +| TTLTriggerBehavior | For TTL triggering, selects Rising Edge or Falling Edge | +| TTLTriggerMode | Select "None" for software control. I have not yet tested other modes, but they should work." | +| TickTime | The UGA 42 has an internal "clock" and operates with sequential time slots ("ticks"), This property sets the duration of a tick in microseconds. Default is 50, minimum is 40, maximum is 1000. | + + +## Installation + +The UGA-42 requires the files "obsTools.dll" and "ROE UGA-42 SDK.DLL" in the micro-manager installation directory. The code was build agains UGA-42 SDK_1139, and it can not be guaranteed that other versions will work. If you do not have these files, please contact Rapp support and request them. The Rapp UGA42 should be auto-detected. Leave the debug property set to false (setting it to true will dramatizally slow down communication with the device). This device adapter currently only supports one UGA42. + + +## See Also + +- [Rapp](Rapp) - Original adapter for the UGA-40 +- [RappLasers](RappLasers) - Lasers for use with the UGA42. You may need these to open the safety shutter of your Rapp Lasers and to set the laser into RMI-Mix controlmode. +- [Projector Plugin](Projector_Plugin) - For interactive phototargeting + +