diff --git a/change/react-native-windows-2020-02-25-11-32-44-tabindex.json b/change/react-native-windows-2020-02-25-11-32-44-tabindex.json
new file mode 100644
index 00000000000..29082acf039
--- /dev/null
+++ b/change/react-native-windows-2020-02-25-11-32-44-tabindex.json
@@ -0,0 +1,9 @@
+{
+  "type": "prerelease",
+  "comment": "Set IsTabStop to false when tabIndex is negative",
+  "packageName": "react-native-windows",
+  "email": "lamdoan@microsoft.com",
+  "commit": "27a16dc9783aefa04c5035c4c2a4b39e8d11f6f8",
+  "dependentChangeType": "patch",
+  "date": "2020-02-25T19:32:43.976Z"
+}
\ No newline at end of file
diff --git a/vnext/ReactUWP/Views/ControlViewManager.cpp b/vnext/ReactUWP/Views/ControlViewManager.cpp
index 579a1cbe515..e0bbc4fc1e4 100644
--- a/vnext/ReactUWP/Views/ControlViewManager.cpp
+++ b/vnext/ReactUWP/Views/ControlViewManager.cpp
@@ -64,8 +64,15 @@ void ControlViewManager::UpdateProperties(ShadowNodeBase *nodeToUpdate, const fo
       } else if (propertyName == "tabIndex") {
         if (propertyValue.isNumber()) {
           auto tabIndex = propertyValue.asDouble();
-          if (tabIndex == static_cast<int32_t>(tabIndex))
-            control.TabIndex(static_cast<int32_t>(tabIndex));
+          if (tabIndex == static_cast<int32_t>(tabIndex)) {
+            if (tabIndex < 0) {
+              control.IsTabStop(false);
+              control.ClearValue(winrt::Control::TabIndexProperty());
+            } else {
+              control.IsTabStop(true);
+              control.TabIndex(static_cast<int32_t>(tabIndex));
+            }
+          }
         } else if (propertyValue.isNull()) {
           control.ClearValue(winrt::Control::TabIndexProperty());
         }
diff --git a/vnext/ReactUWP/Views/ViewViewManager.cpp b/vnext/ReactUWP/Views/ViewViewManager.cpp
index adcf4824ac4..1a19bb24260 100644
--- a/vnext/ReactUWP/Views/ViewViewManager.cpp
+++ b/vnext/ReactUWP/Views/ViewViewManager.cpp
@@ -87,8 +87,15 @@ class ViewShadowNode : public ShadowNodeBase {
   void TabIndex(int32_t tabIndex) {
     m_tabIndex = tabIndex;
 
-    if (IsControl())
-      GetControl().TabIndex(m_tabIndex);
+    if (IsControl()) {
+      if (tabIndex < 0) {
+        GetControl().IsTabStop(false);
+        GetControl().ClearValue(winrt::Control::TabIndexProperty());
+      } else {
+        GetControl().IsTabStop(true);
+        GetControl().TabIndex(tabIndex);
+      }
+    }
   }
 
   bool OnClick() {
@@ -206,7 +213,7 @@ class ViewShadowNode : public ShadowNodeBase {
 
   bool m_enableFocusRing = true;
   bool m_onClick = false;
-  int32_t m_tabIndex = std::numeric_limits<std::int32_t>::max();
+  int32_t m_tabIndex = -1;
 
   winrt::ContentControl::GotFocus_revoker m_contentControlGotFocusRevoker{};
   winrt::ContentControl::LostFocus_revoker m_contentControlLostFocusRevoker{};