diff --git a/src/Adapter/MSTestAdapter.PlatformServices/Helpers/AssemblyFileLocator.cs b/src/Adapter/MSTestAdapter.PlatformServices/Helpers/AssemblyFileLocator.cs
new file mode 100644
index 0000000000..0939fe644b
--- /dev/null
+++ b/src/Adapter/MSTestAdapter.PlatformServices/Helpers/AssemblyFileLocator.cs
@@ -0,0 +1,71 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT license. See LICENSE file in the project root for full license information.
+
+namespace Microsoft.VisualStudio.TestPlatform.MSTestAdapter.PlatformServices.Helpers;
+
+/// <summary>
+/// Centralizes access to <see cref="Assembly.Location"/> so that single-file / Native AOT
+/// scenarios (where <c>Assembly.Location</c> returns an empty string) are handled once instead
+/// of being scattered across many call sites with individual <c>IL3000</c> suppressions.
+/// </summary>
+/// <remarks>
+/// All members tolerate <see cref="Assembly.Location"/> returning an empty string — that is the
+/// documented behavior for assemblies embedded in single-file or Native AOT executables.
+/// Callers receive <see langword="null"/> (for try-style getters) or fall back to
+/// <see cref="AppContext.BaseDirectory"/> / the assembly simple name when appropriate.
+/// </remarks>
+internal static class AssemblyFileLocator
+{
+    /// <summary>
+    /// Returns the file path of the given assembly, or <see langword="null"/> when the
+    /// assembly is embedded in a single-file or Native AOT executable (and therefore has no
+    /// on-disk location).
+    /// </summary>
+    [UnconditionalSuppressMessage("SingleFile", "IL3000:Avoid accessing Assembly file path when publishing as a single file", Justification = "Empty return is the documented contract; callers are expected to handle null.")]
+    public static string? TryGetLocation(Assembly assembly)
+    {
+        string location = assembly.Location;
+        return string.IsNullOrEmpty(location) ? null : location;
+    }
+
+    /// <summary>
+    /// Returns the directory containing the given assembly, falling back to
+    /// <see cref="AppContext.BaseDirectory"/> when <see cref="Assembly.Location"/> is empty
+    /// (single-file / Native AOT).
+    /// </summary>
+    [UnconditionalSuppressMessage("SingleFile", "IL3000:Avoid accessing Assembly file path when publishing as a single file", Justification = "Falls back to AppContext.BaseDirectory when Assembly.Location is empty (single-file/Native AOT case).")]
+    public static string GetDirectoryOrAppContextBase(Assembly assembly)
+    {
+        string location = assembly.Location;
+        if (!string.IsNullOrEmpty(location))
+        {
+            string? directory = Path.GetDirectoryName(location);
+            if (!string.IsNullOrEmpty(directory))
+            {
+                return directory;
+            }
+        }
+
+        return AppContext.BaseDirectory;
+    }
+
+    /// <summary>
+    /// Returns the file name (with extension) of the given assembly, falling back to the
+    /// assembly simple name with a <c>.dll</c> suffix when <see cref="Assembly.Location"/>
+    /// is empty (single-file / Native AOT).
+    /// </summary>
+    [UnconditionalSuppressMessage("SingleFile", "IL3000:Avoid accessing Assembly file path when publishing as a single file", Justification = "Falls back to assembly simple name when Assembly.Location is empty (single-file/Native AOT case).")]
+    public static string GetFileNameOrSimpleName(Assembly assembly)
+    {
+        string location = assembly.Location;
+        if (!string.IsNullOrEmpty(location))
+        {
+            return Path.GetFileName(location);
+        }
+
+        string? simpleName = assembly.GetName().Name;
+        return string.IsNullOrEmpty(simpleName)
+            ? string.Empty
+            : simpleName + ".dll";
+    }
+}
diff --git a/src/Adapter/MSTestAdapter.PlatformServices/Helpers/DataSerializationHelper.cs b/src/Adapter/MSTestAdapter.PlatformServices/Helpers/DataSerializationHelper.cs
index b848f88591..45f7f92133 100644
--- a/src/Adapter/MSTestAdapter.PlatformServices/Helpers/DataSerializationHelper.cs
+++ b/src/Adapter/MSTestAdapter.PlatformServices/Helpers/DataSerializationHelper.cs
@@ -35,6 +35,14 @@ internal static class DataSerializationHelper
     /// </summary>
     /// <param name="data">Data array to serialize.</param>
     /// <returns>Serialized array.</returns>
+    /// <exception cref="NotSupportedException">
+    /// Thrown when the runtime does not support dynamic code generation
+    /// (<c>RuntimeFeature.IsDynamicCodeSupported</c> is <see langword="false"/>),
+    /// for example under Native AOT, Mono AOT on iOS, or Blazor WebAssembly AOT.
+    /// DataContract-based serialization relies on dynamic code generation, and in such
+    /// AOT/MTP scenarios the in-process <c>ActualData</c> reference is used instead,
+    /// so this method should never be reached at runtime.
+    /// </exception>
     [UnconditionalSuppressMessage("ReflectionAnalysis", "IL2026:Members attributed with RequiresUnreferencedCode may break when trimming", Justification = DataContractSerializationJustification)]
     [UnconditionalSuppressMessage("Aot", "IL3050:Avoid calling members annotated with 'RequiresDynamicCodeAttribute' when publishing as Native AOT", Justification = DataContractSerializationJustification)]
     public static string?[]? Serialize(object?[]? data)
@@ -44,6 +52,22 @@ internal static class DataSerializationHelper
             return null;
         }
 
+#if NET
+        if (!RuntimeFeature.IsDynamicCodeSupported)
+        {
+            // Cross-process data serialization is not used when dynamic code generation is
+            // unavailable (Native AOT, Mono iOS AOT, Blazor WASM AOT, ...). In MTP mode,
+            // discovery sets ActualData (in-process reference) instead. Reaching this code
+            // means a vstest-style code path is being exercised in an AOT build, which is
+            // unsupported because DataContractJsonSerializer requires runtime code generation.
+            throw new NotSupportedException(
+                "MSTest data-source argument serialization is not supported when the runtime " +
+                "does not support dynamic code generation (Native AOT, Mono iOS AOT, Blazor " +
+                "WebAssembly AOT, ...). Use Microsoft.Testing.Platform (MTP) mode, where " +
+                "parameterized test arguments are passed in-process.");
+        }
+#endif
+
         string?[] serializedData = new string?[data.Length * 2];
         for (int i = 0; i < data.Length; i++)
         {
@@ -82,6 +106,14 @@ internal static class DataSerializationHelper
     /// </summary>
     /// <param name="serializedData">Serialized data array to deserialize.</param>
     /// <returns>Deserialized array.</returns>
+    /// <exception cref="NotSupportedException">
+    /// Thrown when the runtime does not support dynamic code generation
+    /// (<c>RuntimeFeature.IsDynamicCodeSupported</c> is <see langword="false"/>),
+    /// for example under Native AOT, Mono AOT on iOS, or Blazor WebAssembly AOT.
+    /// DataContract-based deserialization relies on dynamic code generation, and in such
+    /// AOT/MTP scenarios the in-process <c>ActualData</c> reference is used instead,
+    /// so this method should never be reached at runtime.
+    /// </exception>
     [UnconditionalSuppressMessage("ReflectionAnalysis", "IL2026:Members attributed with RequiresUnreferencedCode may break when trimming", Justification = DataContractSerializationJustification)]
     [UnconditionalSuppressMessage("Aot", "IL3050:Avoid calling members annotated with 'RequiresDynamicCodeAttribute' when publishing as Native AOT", Justification = DataContractSerializationJustification)]
     public static object?[]? Deserialize(string?[]? serializedData)
@@ -91,6 +123,20 @@ internal static class DataSerializationHelper
             return null;
         }
 
+#if NET
+        if (!RuntimeFeature.IsDynamicCodeSupported)
+        {
+            // See note on Serialize: when dynamic code generation is unavailable
+            // (Native AOT, Mono iOS AOT, Blazor WASM AOT, ...), the execution path uses
+            // TestMethod.ActualData in MTP mode, so this branch should be unreachable.
+            throw new NotSupportedException(
+                "MSTest data-source argument deserialization is not supported when the runtime " +
+                "does not support dynamic code generation (Native AOT, Mono iOS AOT, Blazor " +
+                "WebAssembly AOT, ...). Use Microsoft.Testing.Platform (MTP) mode, where " +
+                "parameterized test arguments are passed in-process.");
+        }
+#endif
+
         int length = serializedData.Length / 2;
         object?[] data = new object?[length];
 
diff --git a/src/Adapter/MSTestAdapter.PlatformServices/Services/TestSourceHost.cs b/src/Adapter/MSTestAdapter.PlatformServices/Services/TestSourceHost.cs
index 60f818f82f..7ff1be7380 100644
--- a/src/Adapter/MSTestAdapter.PlatformServices/Services/TestSourceHost.cs
+++ b/src/Adapter/MSTestAdapter.PlatformServices/Services/TestSourceHost.cs
@@ -18,6 +18,9 @@
 #if !WINDOWS_UWP
 using Microsoft.VisualStudio.TestTools.UnitTesting;
 #endif
+#if NETFRAMEWORK || (NET && !WINDOWS_UWP)
+using Microsoft.VisualStudio.TestPlatform.MSTestAdapter.PlatformServices.Helpers;
+#endif
 
 namespace Microsoft.VisualStudio.TestPlatform.MSTestAdapter.PlatformServices;
 
@@ -110,21 +113,32 @@ internal TestSourceHost(string sourceFileName, IRunSettings? runSettings, IAppDo
     public void SetupHost()
     {
 #if NET && !WINDOWS_UWP
-        List<string> resolutionPaths = GetResolutionPaths(_sourceFileName, false);
-
-        if (PlatformServiceProvider.Instance.AdapterTraceLogger.IsInfoEnabled)
+        // When the runtime does not support dynamic code generation
+        // (RuntimeFeature.IsDynamicCodeSupported is false — Native AOT, Mono iOS AOT,
+        // Blazor WebAssembly AOT, ...), the host cannot load assemblies through
+        // reflection-style fallbacks, so the AssemblyResolver (which hooks
+        // AssemblyLoadContext.Resolving to load assemblies on demand) can never
+        // contribute anything useful. Skip its creation entirely; this also lets the
+        // trimmer drop GetResolutionPaths and the resolver itself from the published
+        // binary.
+        if (RuntimeFeature.IsDynamicCodeSupported)
         {
-            PlatformServiceProvider.Instance.AdapterTraceLogger.Info("DesktopTestSourceHost.SetupHost(): Creating assembly resolver with resolution paths {0}.", string.Join(',', resolutionPaths));
-        }
+            List<string> resolutionPaths = GetResolutionPaths(_sourceFileName, false);
 
-        var assemblyResolver = new AssemblyResolver(resolutionPaths);
-        if (TryAddSearchDirectoriesSpecifiedInRunSettingsToAssemblyResolver(assemblyResolver, Path.GetDirectoryName(_sourceFileName)!))
-        {
-            _parentDomainAssemblyResolver = assemblyResolver;
-        }
-        else
-        {
-            assemblyResolver.Dispose();
+            if (PlatformServiceProvider.Instance.AdapterTraceLogger.IsInfoEnabled)
+            {
+                PlatformServiceProvider.Instance.AdapterTraceLogger.Info("DesktopTestSourceHost.SetupHost(): Creating assembly resolver with resolution paths {0}.", string.Join(',', resolutionPaths));
+            }
+
+            var assemblyResolver = new AssemblyResolver(resolutionPaths);
+            if (TryAddSearchDirectoriesSpecifiedInRunSettingsToAssemblyResolver(assemblyResolver, Path.GetDirectoryName(_sourceFileName)!))
+            {
+                _parentDomainAssemblyResolver = assemblyResolver;
+            }
+            else
+            {
+                assemblyResolver.Dispose();
+            }
         }
 #elif NETFRAMEWORK
         List<string> resolutionPaths = GetResolutionPaths(_sourceFileName, VSInstallationUtilities.IsCurrentProcessRunningInPortableMode());
@@ -272,7 +286,7 @@ private void SetContext(string? source)
 #if WIN_UI
             if (StringEx.IsNullOrEmpty(dirName))
             {
-                dirName = Path.GetDirectoryName(typeof(TestSourceHost).Assembly.Location)!;
+                dirName = AssemblyFileLocator.GetDirectoryOrAppContextBase(typeof(TestSourceHost).Assembly);
             }
 
             Directory.SetCurrentDirectory(dirName);
@@ -326,8 +340,8 @@ internal string GetAppBaseAsPerPlatform()
         //    there would be a mismatch of platform service assemblies during discovery.
         DebugEx.Assert(_targetFrameworkVersion is not null, "Target framework version is null.");
         return _targetFrameworkVersion.Contains(EngineConstants.DotNetFrameWorkStringPrefix)
-            ? Path.GetDirectoryName(_sourceFileName) ?? Path.GetDirectoryName(typeof(TestSourceHost).Assembly.Location)
-            : Path.GetDirectoryName(typeof(TestSourceHost).Assembly.Location);
+            ? Path.GetDirectoryName(_sourceFileName) ?? AssemblyFileLocator.GetDirectoryOrAppContextBase(typeof(TestSourceHost).Assembly)
+            : AssemblyFileLocator.GetDirectoryOrAppContextBase(typeof(TestSourceHost).Assembly);
     }
 
     internal string GetTargetFrameworkVersionString(string sourceFileName)
@@ -350,7 +364,6 @@ internal string GetTargetFrameworkVersionString(string sourceFileName)
     /// <returns>
     /// A list of path.
     /// </returns>
-    [UnconditionalSuppressMessage("SingleFile", "IL3000:Avoid accessing Assembly file path when publishing as a single file", Justification = "Assembly.Location is explicitly checked for empty before use; in single-file/Native AOT scenarios it returns empty and the affected blocks are skipped.")]
     internal virtual List<string> GetResolutionPaths(string sourceFileName, bool isPortableMode)
     {
         List<string> resolutionPaths =
@@ -379,24 +392,22 @@ internal virtual List<string> GetResolutionPaths(string sourceFileName, bool isP
 #endif
         }
 
-        // We check for the empty path, and in single file mode, or on source gen mode we don't allow
-        // loading dependencies than from the current folder, which is what the default loader handles by itself.
-        if (!string.IsNullOrEmpty(typeof(TestSourceHost).Assembly.Location))
+        // AssemblyFileLocator.TryGetLocation returns null when Assembly.Location is empty
+        // (single-file / Native AOT). In those scenarios we don't add the adapter or test platform
+        // directories to the resolution paths and rely on the default loader resolving dependencies
+        // from the current folder, which is what it handles by itself in single-file mode.
+        string? adapterDirectory = Path.GetDirectoryName(AssemblyFileLocator.TryGetLocation(typeof(TestSourceHost).Assembly));
+        if (!string.IsNullOrEmpty(adapterDirectory) && !resolutionPaths.Contains(adapterDirectory))
         {
             // Adding adapter folder to resolution paths
-            if (!resolutionPaths.Contains(Path.GetDirectoryName(typeof(TestSourceHost).Assembly.Location)!))
-            {
-                resolutionPaths.Add(Path.GetDirectoryName(typeof(TestSourceHost).Assembly.Location)!);
-            }
+            resolutionPaths.Add(adapterDirectory);
         }
 
-        if (!string.IsNullOrEmpty(typeof(AssemblyHelper).Assembly.Location))
+        string? testPlatformDirectory = Path.GetDirectoryName(AssemblyFileLocator.TryGetLocation(typeof(AssemblyHelper).Assembly));
+        if (!string.IsNullOrEmpty(testPlatformDirectory) && !resolutionPaths.Contains(testPlatformDirectory))
         {
             // Adding TestPlatform folder to resolution paths
-            if (!resolutionPaths.Contains(Path.GetDirectoryName(typeof(AssemblyHelper).Assembly.Location)!))
-            {
-                resolutionPaths.Add(Path.GetDirectoryName(typeof(AssemblyHelper).Assembly.Location)!);
-            }
+            resolutionPaths.Add(testPlatformDirectory);
         }
 
         return resolutionPaths;
diff --git a/src/Adapter/MSTestAdapter.PlatformServices/Utilities/DeploymentUtilityBase.cs b/src/Adapter/MSTestAdapter.PlatformServices/Utilities/DeploymentUtilityBase.cs
index dd6918a934..be6335ba69 100644
--- a/src/Adapter/MSTestAdapter.PlatformServices/Utilities/DeploymentUtilityBase.cs
+++ b/src/Adapter/MSTestAdapter.PlatformServices/Utilities/DeploymentUtilityBase.cs
@@ -6,6 +6,7 @@
 using Microsoft.VisualStudio.TestPlatform.MSTest.TestAdapter;
 using Microsoft.VisualStudio.TestPlatform.MSTestAdapter.PlatformServices.Deployment;
 using Microsoft.VisualStudio.TestPlatform.MSTestAdapter.PlatformServices.Extensions;
+using Microsoft.VisualStudio.TestPlatform.MSTestAdapter.PlatformServices.Helpers;
 
 using Microsoft.VisualStudio.TestPlatform.ObjectModel;
 using Microsoft.VisualStudio.TestPlatform.ObjectModel.Adapter;
@@ -136,7 +137,6 @@ public static string GetTestResultsDirectory(IRunContext? runContext) => !String
     /// <param name="deploymentDirectory">The deployment directory.</param>
     /// <param name="resultsDirectory">Root results directory.</param>
     /// <returns>Returns a list of deployment warnings.</returns>
-    [UnconditionalSuppressMessage("SingleFile", "IL3000:Avoid accessing Assembly file path when publishing as a single file", Justification = "Deployment is a reflection-mode/legacy adapter feature; in single-file/Native AOT scenarios Assembly.Location returns an empty string and the comparison falls through without error.")]
     protected IEnumerable<string> Deploy(IList<DeploymentItem> deploymentItems, string testSourceHandler, string deploymentDirectory, string resultsDirectory)
     {
         Ensure.NotNullOrWhiteSpace(deploymentDirectory);
@@ -194,8 +194,9 @@ protected IEnumerable<string> Deploy(IList<DeploymentItem> deploymentItems, stri
 
                     // Ignore the test platform files.
                     string tempFile = Path.GetFileName(fileToDeploy);
-                    // We throw when we run in source gen mode.
-                    string assemblyName = Path.GetFileName(GetType().Assembly.Location);
+                    // Use AssemblyFileLocator to safely obtain the file name even when Assembly.Location
+                    // is empty (single-file / Native AOT scenarios) by falling back to the simple name.
+                    string assemblyName = AssemblyFileLocator.GetFileNameOrSimpleName(GetType().Assembly);
                     if (tempFile.Equals(assemblyName, StringComparison.OrdinalIgnoreCase))
                     {
                         continue;