Analysis date: Sat Mar 16 23:38:18 CET 2024
Input file(s): barcode09.fastq
Output file(s): barcode09_mixcr_results/barcode09_result.vdjca
Version: 4.6.0; built=Sat Dec 09 20:48:42 CET 2023; rev=c9fafa41fe; lib=repseqio.v4.0
Command line arguments: align --report barcode09_mixcr_results/barcode09_result.align.report.txt --json-report barcode09_mixcr_results/barcode09_result.align.report.json --preset generic-ont --save-output-file-names barcode09_mixcr_results/barcode09_result.align.list --species dog --library imgt barcode09.fastq barcode09_mixcr_results/barcode09_result.vdjca
Analysis time: 23.88s
Total sequencing reads: 791960
Successfully aligned reads: 105197 (13.28%)
Coverage (percent of successfully aligned):
  CDR3: 97555 (92.74%)
  FR3_TO_FR4: 41710 (39.65%)
  CDR2_TO_FR4: 101778 (96.75%)
  FR2_TO_FR4: 101063 (96.07%)
  CDR1_TO_FR4: 100658 (95.69%)
  VDJRegion: 101667 (96.64%)
Alignment failed: no hits (not TCR/IG?): 625559 (78.99%)
Alignment failed: absence of J hits: 61204 (7.73%)
Overlapped: 0 (0%)
Overlapped and aligned: 0 (0%)
Overlapped and not aligned: 0 (0%)
Alignment-aided overlaps, percent of overlapped and aligned: 0 (NaN%)
No CDR3 parts alignments, percent of successfully aligned: 2444 (2.32%)
Partial aligned reads, percent of successfully aligned: 5198 (4.94%)
Realigned with forced non-floating bound: 0 (0%)
Realigned with forced non-floating right bound in left read: 0 (0%)
Realigned with forced non-floating left bound in right read: 0 (0%)
IGH chains: 39518 (37.57%)
IGH non-functional: 6950 (17.59%)
IGK chains: 1 (0%)
IGK non-functional: 0 (0%)
IGL chains: 65678 (62.43%)
IGL non-functional: 8708 (13.26%)
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