Modified version of MultiStackRegistration, for use from scripts.
- Brad Busse (Designer, Developer, - v 1.45)
- Kota Miura (Minor upgrades, v1.46)
- Mavenized, Scripting enabled, bug fixing.
Welcome to MultiStackReg v1.4!
This plugin has three modes of use:
- Align a single stack of images
- Align a stack to another stack
- Load a previously created transformation file
To align a single stack:
Choose the image to be aligned in the Stack 1 dropdown box. Leave 'Align' in the Action 1 box.
To align two stacks:
Place the reference stack in Stack 1's box, and the stack to be aligned in Stack 2's box. Select 'Use as Reference' as Action 1 and 'Align to First Stack' as Action 2.
To load a transformation file:
Place the stack to be aligned in either stack box, choose 'Load Transformation File' in its Action dropdown box. The File field can be used as a shortcut to avoid having to select the matrix manually.
This work is based on the following paper:
P. Thévenaz, U.E. Ruttimann, M. Unser A Pyramid Approach to Subpixel Registration Based on Intensity IEEE Transactions on Image Processing vol. 7, no. 1, pp. 27-41, January 1998.
This paper is available on-line at
Other relevant on-line publications are available at
Additional help available at
Ancillary TurboReg_ plugin available at
You'll be free to use this software for research purposes, but you should not redistribute it without our consent. In addition, we expect you to include a citation or acknowledgment whenever you present or publish results that are based on it.
A few changes (loadTransform, appendTransform, multi stack support) to support load/save functionality and multiple stacks were added by Brad Busse ( firstname.lastname@example.org ) and released into the public domain, so go by their ^^ guidelines for distribution, etc.