diff --git a/mlrun/runtimes/base.py b/mlrun/runtimes/base.py
index 9b327a5ca49..e8b1e3a127f 100644
--- a/mlrun/runtimes/base.py
+++ b/mlrun/runtimes/base.py
@@ -792,7 +792,7 @@ def with_requirements(self, requirements: Union[str, List[str]]):
         """
         if isinstance(requirements, str):
             with open(requirements, "r") as fp:
-                requirements = fp.readlines()
+                requirements = fp.read().splitlines()
         commands = self.spec.build.commands or []
         commands.append("python -m pip install " + " ".join(requirements))
         self.spec.build.commands = commands
diff --git a/tests/api/runtimes/assets/requirements.txt b/tests/api/runtimes/assets/requirements.txt
new file mode 100644
index 00000000000..cfb01f5a40f
--- /dev/null
+++ b/tests/api/runtimes/assets/requirements.txt
@@ -0,0 +1,2 @@
+faker
+python-dotenv
\ No newline at end of file
diff --git a/tests/api/runtimes/base.py b/tests/api/runtimes/base.py
index ab3adf5c9f6..f085c6f354f 100644
--- a/tests/api/runtimes/base.py
+++ b/tests/api/runtimes/base.py
@@ -34,6 +34,7 @@ def setup_method(self, method):
         self.artifact_path = "/tmp"
         self.function_name_label = "mlrun/name"
         self.code_filename = str(self.assets_path / "sample_function.py")
+        self.requirements_file = str(self.assets_path / "requirements.txt")
 
         self.vault_secrets = ["secret1", "secret2", "AWS_KEY"]
         self.vault_secret_value = "secret123!@"
diff --git a/tests/api/runtimes/test_kubejob.py b/tests/api/runtimes/test_kubejob.py
index 772b2e99011..f5e26fb0192 100644
--- a/tests/api/runtimes/test_kubejob.py
+++ b/tests/api/runtimes/test_kubejob.py
@@ -1,5 +1,6 @@
 import os
 
+import deepdiff
 import pytest
 from fastapi.testclient import TestClient
 from sqlalchemy.orm import Session
@@ -241,3 +242,14 @@ def test_set_label(self, db: Session, client: TestClient):
         runtime = self._generate_runtime()
         self._execute_run(runtime, runspec=task)
         self._assert_pod_creation_config(expected_labels=labels)
+
+    def test_with_requirements(self, db: Session, client: TestClient):
+        runtime = self._generate_runtime()
+        runtime.with_requirements(self.requirements_file)
+        expected_commands = ["python -m pip install faker python-dotenv"]
+        assert (
+            deepdiff.DeepDiff(
+                expected_commands, runtime.spec.build.commands, ignore_order=True,
+            )
+            == {}
+        )