ncbp-pipeline

Data are available via ProteomeXchange with identifier PXD016038.

contact moghbaie@rockefeller.edu for any questions

12/27/2019

Project code and data is organized in following order:

• src
• Input_data
• Output

Schematic representation of interaction screening:

Buffers used for the screens:

Screen	Buffer no.	Buffer	Buffer Concentration	Salt	Salt concentration	Detergent	Detergent Concentration	pH
NCBP1, 2, 3	7	HEPES-Na	20 mM	NaCl	150 mM	d-BCHAP	5mM	7.4
NCBP1, 2, 3	10	HEPES-Na	20 mM	NaCl	300 mM	Sarcosyl	0.5mM	7.4
NCBP1, 2, 3	12	HEPES-Na	20 mM	NaCl	150 mM	TRIT X100	0.5 % v/v	7.4
NCBP1, 2, 3	14	HEPES-Na	20 mM	MgCl2	50 mM	TRIT X100	0.5 % v/v	7.4
NCBP1, 2, 3	18	NH4 Acet	125 mM	NaCl	100 mM	TRIT X100	1 % v/v	7
NCBP1, 2, 3	20	TRIS-Cl	20 mM	Na3 Cit	125 mM	TRIT X100	1 % v/v	8