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Pcr.seqs - allow for forward and reverse diffs #244
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mothur-westcott
added Enhancement Command:Pcr.seqs
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Jun 6, 2016
mothur-westcott
added this to the
Version 1.38.0
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Jun 6, 2016
mothur-westcott
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Jul 14, 2016
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mothur-westcott |
2e89f31
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Completed with commit 2e89f31 |
mothur-westcott
closed this
Jul 14, 2016
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mothur-westcott commentedJun 6, 2016
"Is is possible to set the number of pdiffs separately for the forward and reverse primers? I believe the current method is for the total number of pdiffs? so setting pdiffs = 3 means I'll scrap a sequence with 2 mismatches to both the forward and reverse primer;
fpdiffs=2(match) rpdiffs=2(match)
but setting pdiffs = 6, means that I can get some pass that I do not want to
fpdiffs=1(match) rpdiffs=5(noMatch)
Any reasons as to why this is not good would be appreciated.
Thanks"