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errors in cluster split M ..........is not in your count table #336
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How many unique sequences are you trying to cluster? How much RAM do you have? What version of mothur are you running? You can download our latest version here, https://github.com/mothur/mothur/releases. Version 1.39 includes our new clustering method which creates better quality OTUs while using less time and memory. You can read about it in our paper, http://msphere.asm.org/content/2/2/e00073-17. |
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gracelaimail commentedMay 17, 2017
When I run the
cluster.split(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.uchime.pick.pick.count_table, taxonomy=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pds.wang.pick.taxonomy, splitmethod=classify, taxlevel=4, cutoff=0.15,)
.......................................
after making the dist files, an error show up
Clustering stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta.30.dist
********************###########
Reading matrix: |||||||||||||||||||||||||||||||||||||||||||[ERROR]: M04377_5 _00000M04377_5_000000000-ATAA5_1_1101_16457_2077 is not in your count table. Please correct.
Cutoff was 0.15 changed cutoff to 0.11
Reading stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta.46.dist
********************###########
Reading matrix: |||||||||||||||||||||||||||||||||[ERROR]: M04377_30_0000M04377_30_000000000-B99TL_1_1102_15152_18712 is not in your count table. Please correct.
angel@s1:~/ISF/Samson/mothur$ |||||||[ERROR]: M04377_30_000000000-B99TL_1M04377_ 30_000000000-B99TL_1_2115_9268_2451 is not in your count table. Please correct.
seems the M04377_30_000000000-B99TL_1 is duplicate in the begining.
Any one can help to solve the error ?