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errors in cluster split M ..........is not in your count table #336

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gracelaimail opened this Issue May 17, 2017 · 1 comment

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When I run the

cluster.split(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.uchime.pick.pick.count_table, taxonomy=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pds.wang.pick.taxonomy, splitmethod=classify, taxlevel=4, cutoff=0.15,)
.......................................
after making the dist files, an error show up

Clustering stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta.30.dist
********************###########
Reading matrix: |||||||||||||||||||||||||||||||||||||||||||[ERROR]: M04377_5 _00000M04377_5_000000000-ATAA5_1_1101_16457_2077 is not in your count table. Please correct.
Cutoff was 0.15 changed cutoff to 0.11

Reading stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta.46.dist
********************###########
Reading matrix: |||||||||||||||||||||||||||||||||[ERROR]: M04377_30_0000M04377_30_000000000-B99TL_1_1102_15152_18712 is not in your count table. Please correct.
angel@s1:~/ISF/Samson/mothur$ |||||||[ERROR]: M04377_30_000000000-B99TL_1M04377_ 30_000000000-B99TL_1_2115_9268_2451 is not in your count table. Please correct.

seems the M04377_30_000000000-B99TL_1 is duplicate in the begining.

Any one can help to solve the error ?

Contributor

mothur-westcott commented May 18, 2017

How many unique sequences are you trying to cluster? How much RAM do you have? What version of mothur are you running? You can download our latest version here, https://github.com/mothur/mothur/releases. Version 1.39 includes our new clustering method which creates better quality OTUs while using less time and memory. You can read about it in our paper, http://msphere.asm.org/content/2/2/e00073-17.

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