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Make.sra - [ERROR]: unexpected number of files #338
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mothur-westcott
added the
Command:Make.sra
label
May 22, 2017
mothur-westcott
added this to the
Back Burner
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May 22, 2017
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mothur-westcott commentedMay 22, 2017
BARCODE CAGGCGTATTGG NONE 263DOCGO C1.0.263DOCGO
BARCODE AAGGAGCGCCTT NONE 263DOCGO C1.5.263DOCGO
BARCODE CGATCCGTATTA NONE 263DOCGO C1.6
BARCODE CTATTTGCGACA NONE 263DOCGO C2.5.263DOCGO
BARCODE AGTAGAGGGATG NONE 263DOCGO C2.6
BARCODE GTACATACCGGT NONE 263DOCGO INOCULUM.9
BARCODE GGATCGCAGATC NONE 263DOCGO NH4A.5.263DOCGO
The barcode name contains '.', so mothur is reading 263DOCGOC1.0.263DOCGO as the group name, but when it tries to pair files for groups it finds 263DOCGOC1.0.263DOCGO but then clips the group to the first '.', making the name 263DOCGOC1. Because several file pairs now map to the same group the command is failing. Mothur should be more flexible with this and I will add it to our list. In the meantime if you edit the oligos file to remove the '.' in the group name you should be able to run it.
BARCODE CAGGCGTATTGG NONE 263DOCGO_C1_0.263DOCGO
BARCODE AAGGAGCGCCTT NONE 263DOCGO_C1_5_263DOCGO