ImageJ plugins for blind decomposition of fluorencence microscopy data
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Documentation
ImageJ
SpectralLibrary
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README
README.md

README.md

Blind decomposition of spectrally resolved data – single excitation

Documentation is available as html in the Documentation folder. The plug-In can be found in the ImageJ/NMF folder. Reference: Blind source separation techniques for the decomposition of multiply labeled fluorescence images. RA Neher, M Mitkovski, F Kirchhoff, E Neher, FJ Theis, A Zeug. Biophysical journal 96 (9), 3791-3800

Blind decomposition of spectrally resolved data – multiple excitation

Documentation is available as html in the Documentation folder. The plug-In can be found in the ImageJ/NTF folder. Reference: Blind source separation techniques for the decomposition of multiply labeled fluorescence images. RA Neher, M Mitkovski, F Kirchhoff, E Neher, FJ Theis, A Zeug. Biophysical journal 96 (9), 3791-3800

Optimize your choice of excitation wavelength and spectra channel

Documentation is available as html in the Documentation folder. The plug-In can be found in the ImageJ/OAS folder. Reference: Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. R Neher, E Neher. Journal of microscopy 213 (1), 46-62

Library of spectra

All our plug-ins use a library of spectra. A sample library of spectra is available, mostly by George McNamara.