Skip to content
Automatic calculation of gene expression rate from time-course fluorescence assays on microplate reader (bacterial cells)
MATLAB
Branch: master
Clone or download
Pull request Compare This branch is 4 commits behind GBS-SynBioLab:master.
Fetching latest commit…
Cannot retrieve the latest commit at this time.
Permalink
Type Name Latest commit message Commit time
Failed to load latest commit information.
README.md
fluorescence_module.m
growth_rate_module.m
main_data_analysis.m
vline.m

README.md

Gene-expression-rate-calculator

Author: Nicolas Kylilis PhD

Intended purpose:

Automated data analysis workflow for the calculation of gene expression rate

Suitable for time-course fluorescence assays on microplate-reader for bacterial cells

Experimental design (microplate layout):

Data analysis will only take place correctly if the following microplate layout is applied.

Column 1: Media blank

Column 2: Control cells for growth rate and autofluorescence

Column 3-12: Sample experimental conditions

Rows A-H: Replicates

Data analysis:

Open matlab software and run "growth_rate_module.m" file script. (Instructions for file naming etc can be found in the script)

You can’t perform that action at this time.