diff --git a/boards/nordic/nrf54ls05dk/board.yml b/boards/nordic/nrf54ls05dk/board.yml
index 34133efe38a..a76c0037ebb 100644
--- a/boards/nordic/nrf54ls05dk/board.yml
+++ b/boards/nordic/nrf54ls05dk/board.yml
@@ -9,3 +9,4 @@ board:
     default: "0.0.0"
     revisions:
     - name: "0.0.0"
+    - name: "0.1.0"
diff --git a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b-common.dtsi b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b-common.dtsi
index dc490afe950..46b038a6d76 100644
--- a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b-common.dtsi
+++ b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b-common.dtsi
@@ -7,48 +7,6 @@
 #include "nrf54ls05dk_nrf54ls05b-pinctrl.dtsi"
 
 / {
-	leds {
-		compatible = "gpio-leds";
-
-		led0: led_0 {
-			gpios = <&gpio1 10 GPIO_ACTIVE_HIGH>;
-			label = "Green LED 1";
-		};
-
-		led1: led_1 {
-			gpios = <&gpio0 2 GPIO_ACTIVE_HIGH>;
-			label = "Green LED 2";
-		};
-	};
-
-	buttons {
-		compatible = "gpio-keys";
-
-		button0: button_0 {
-			gpios = <&gpio1 13 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
-			label = "Push button 0";
-			zephyr,code = <INPUT_KEY_0>;
-		};
-
-		button1: button_1 {
-			gpios = <&gpio1 9 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
-			label = "Push button 1";
-			zephyr,code = <INPUT_KEY_1>;
-		};
-
-		button2: button_2 {
-			gpios = <&gpio1 8 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
-			label = "Push button 2";
-			zephyr,code = <INPUT_KEY_2>;
-		};
-
-		button3: button_3 {
-			gpios = <&gpio0 4 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
-			label = "Push button 3";
-			zephyr,code = <INPUT_KEY_3>;
-		};
-	};
-
 	pwmleds {
 		compatible = "pwm-leds";
 
@@ -58,13 +16,7 @@
 	};
 
 	aliases {
-		led0 = &led0;
-		led1 = &led1;
 		pwm-led0 = &pwm_led1;
-		sw0 = &button0;
-		sw1 = &button1;
-		sw2 = &button2;
-		sw3 = &button3;
 		watchdog0 = &wdt30;
 	};
 };
@@ -82,8 +34,3 @@
 	pinctrl-1 = <&pwm20_sleep>;
 	pinctrl-names = "default", "sleep";
 };
-
-&hfpll {
-	/* For now use 64 MHz clock for CPU and fast peripherals. */
-	clock-frequency = <DT_FREQ_M(64)>;
-};
diff --git a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_0_0.overlay b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_0_0.overlay
new file mode 100644
index 00000000000..ee431f7d365
--- /dev/null
+++ b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_0_0.overlay
@@ -0,0 +1,65 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
+ */
+
+#include "nrf54ls05dk_nrf54ls05b-pinctrl.dtsi"
+
+/ {
+	leds {
+		compatible = "gpio-leds";
+
+		led0: led_0 {
+			gpios = <&gpio1 10 GPIO_ACTIVE_HIGH>;
+			label = "Green LED 1";
+		};
+
+		led1: led_1 {
+			gpios = <&gpio0 2 GPIO_ACTIVE_HIGH>;
+			label = "Green LED 2";
+		};
+	};
+
+	buttons {
+		compatible = "gpio-keys";
+
+		button0: button_0 {
+			gpios = <&gpio1 13 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 0";
+			zephyr,code = <INPUT_KEY_0>;
+		};
+
+		button1: button_1 {
+			gpios = <&gpio1 9 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 1";
+			zephyr,code = <INPUT_KEY_1>;
+		};
+
+		button2: button_2 {
+			gpios = <&gpio1 8 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 2";
+			zephyr,code = <INPUT_KEY_2>;
+		};
+
+		button3: button_3 {
+			gpios = <&gpio0 4 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 3";
+			zephyr,code = <INPUT_KEY_3>;
+		};
+	};
+
+	aliases {
+		led0 = &led0;
+		led1 = &led1;
+		sw0 = &button0;
+		sw1 = &button1;
+		sw2 = &button2;
+		sw3 = &button3;
+	};
+};
+
+&hfpll {
+	/* For now use 64 MHz clock for CPU and fast peripherals. */
+	clock-frequency = <DT_FREQ_M(64)>;
+};
diff --git a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp.yaml b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_0_0.yaml
similarity index 72%
rename from boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp.yaml
rename to boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_0_0.yaml
index 27197df5cd6..19fa82fcfee 100644
--- a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp.yaml
+++ b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_0_0.yaml
@@ -1,8 +1,8 @@
 # Copyright (c) 2025 Nordic Semiconductor ASA
 # SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
 
-identifier: nrf54ls05dk/nrf54ls05b/cpuapp
-name: nRF54LS05-DK-nRF54LS05B-Application
+identifier: nrf54ls05dk@0.0.0/nrf54ls05b/cpuapp
+name: nRF54LS05-DK-nRF54LS05B-Application (rev. 0.0.0)
 type: mcu
 arch: arm
 toolchain:
diff --git a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_1_0.overlay b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_1_0.overlay
new file mode 100644
index 00000000000..f52c89e20d8
--- /dev/null
+++ b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_1_0.overlay
@@ -0,0 +1,72 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
+ */
+
+#include "nrf54ls05dk_nrf54ls05b-pinctrl.dtsi"
+
+/ {
+	leds {
+		compatible = "gpio-leds";
+
+		led0: led_0 {
+			gpios = <&gpio1 15 GPIO_ACTIVE_HIGH>;
+			label = "Green LED 0";
+		};
+
+		led1: led_1 {
+			gpios = <&gpio1 10 GPIO_ACTIVE_HIGH>;
+			label = "Green LED 1";
+		};
+
+		led2: led_2 {
+			gpios = <&gpio1 16 GPIO_ACTIVE_HIGH>;
+			label = "Green LED 2";
+		};
+
+		led3: led_3 {
+			gpios = <&gpio1 14 GPIO_ACTIVE_HIGH>;
+			label = "Green LED 3";
+		};
+	};
+
+	buttons {
+		compatible = "gpio-keys";
+
+		button0: button_0 {
+			gpios = <&gpio1 13 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 0";
+			zephyr,code = <INPUT_KEY_0>;
+		};
+
+		button1: button_1 {
+			gpios = <&gpio1 9 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 1";
+			zephyr,code = <INPUT_KEY_1>;
+		};
+
+		button2: button_2 {
+			gpios = <&gpio1 8 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 2";
+			zephyr,code = <INPUT_KEY_2>;
+		};
+
+		button3: button_3 {
+			gpios = <&gpio0 4 (GPIO_PULL_UP | GPIO_ACTIVE_LOW)>;
+			label = "Push button 3";
+			zephyr,code = <INPUT_KEY_3>;
+		};
+	};
+
+	aliases {
+		led0 = &led0;
+		led1 = &led1;
+		led2 = &led2;
+		led3 = &led3;
+		sw0 = &button0;
+		sw1 = &button1;
+		sw2 = &button2;
+		sw3 = &button3;
+	};
+};
diff --git a/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_1_0.yaml b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_1_0.yaml
new file mode 100644
index 00000000000..1369cc98697
--- /dev/null
+++ b/boards/nordic/nrf54ls05dk/nrf54ls05dk_nrf54ls05b_cpuapp_0_1_0.yaml
@@ -0,0 +1,21 @@
+# Copyright (c) 2025 Nordic Semiconductor ASA
+# SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
+
+identifier: nrf54ls05dk@0.1.0/nrf54ls05b/cpuapp
+name: nRF54LS05-DK-nRF54LS05B-Application (rev. 0.1.0)
+type: mcu
+arch: arm
+toolchain:
+  - gnuarmemb
+  - zephyr
+sysbuild: true
+ram: 96
+flash: 508
+supported:
+  - adc
+  - counter
+  - gpio
+  - i2c
+  - pwm
+  - spi
+  - watchdog