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NTR: Precision nuclear run-on and sequencing assay #876

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jahilton opened this Issue Nov 7, 2017 · 3 comments

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jahilton commented Nov 7, 2017

editor preferred term: Precision nuclear run-on and sequencing assay
alternate terms: PRO-seq
textual definition: An assay to map the genome-wide distribution of transcriptionally-engaged Pol II at base-pair resolution by using biotin-labeled ribonucleotide triphosphate analogs (biotin-NTP) for nuclear run-on reactions, allowing the efficient affinity purification of nascent RNAs for high throughput sequencing from their 3’ ends.
definition source for the textual definition: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974810/
logical definition: RNA-seq assay [OBI:0001271] --> Precision nuclear run-on and sequencing assay

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rctauber Nov 27, 2017

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I have also been seeing it called “precision nuclear run-on sequencing assay” (without the ‘and’), though I can’t tell which one is more common. One should be added as another alt term though.

For definition:

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rctauber commented Nov 27, 2017

I have also been seeing it called “precision nuclear run-on sequencing assay” (without the ‘and’), though I can’t tell which one is more common. One should be added as another alt term though.

For definition:

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zhengj2007 Nov 27, 2017

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Discussed on OBI 2017-11-27. @rctauber will add the term in the assay template.

RT and PCR steps can be added as has_part process in the template.

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zhengj2007 commented Nov 27, 2017

Discussed on OBI 2017-11-27. @rctauber will add the term in the assay template.

RT and PCR steps can be added as has_part process in the template.

jamesaoverton added a commit that referenced this issue May 1, 2018

Merge pull request #918 from rctauber/master
Add new terms for jahilton (#828, #875, #876)
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rctauber Aug 17, 2018

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Resolved by 984887b

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rctauber commented Aug 17, 2018

Resolved by 984887b

@rctauber rctauber closed this Aug 17, 2018

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