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NTR: Clone-seq #877

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jahilton opened this Issue Nov 7, 2017 · 3 comments

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jahilton commented Nov 7, 2017

editor preferred term: Clone-seq
textual definition: A massively-parallel site-directed mutagenesis approach leveraging next-generation sequencing to rapidly and cost-effectively generate a large number of mutant alleles. One colony of each mutagenesis attempt is put into one pool and combine multiple pools through multiplexing for one Illumina sequencing run.
definition source for the textual definition: http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004819
logical definition: sequencing assay --> Clone-seq

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DanBerrios Dec 3, 2017

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I'm wondering if this is more of an experimental design, rather than a truly novel subtype of sequencing assay. The goal of "Clone-Seq" is "to rapidly and cost-effectively generate a large number of mutant alleles", rather than simply to assay a particular single sample for some feature of that sample. The equipment used to perform the sequencing aspects of "Clone-seq" is standard Illumina equipment. The novel portions are combining site-directed mutagenesis techniques and protein-protein interactome information to generate mutants, then organizing those mutants across standard high-throughput sequencing runs.

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DanBerrios commented Dec 3, 2017

I'm wondering if this is more of an experimental design, rather than a truly novel subtype of sequencing assay. The goal of "Clone-Seq" is "to rapidly and cost-effectively generate a large number of mutant alleles", rather than simply to assay a particular single sample for some feature of that sample. The equipment used to perform the sequencing aspects of "Clone-seq" is standard Illumina equipment. The novel portions are combining site-directed mutagenesis techniques and protein-protein interactome information to generate mutants, then organizing those mutants across standard high-throughput sequencing runs.

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DanBerrios Oct 15, 2018

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@jahilton Do you agree?

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DanBerrios commented Oct 15, 2018

@jahilton Do you agree?

@DanBerrios DanBerrios assigned jahilton and unassigned DanBerrios Oct 15, 2018

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bpeters42 Oct 16, 2018

bpeters42 commented Oct 16, 2018

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