diff --git a/CHANGELOG.md b/CHANGELOG.md
index 74f14b26dab..b5f38075690 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,6 +13,7 @@ This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.htm
 - Add `NewMiddleware` function in `go.opentelemetry.io/contrib/instrumentation/net/http/otelhttp`. (#2964)
 - Add the new `go.opentelemetry.io/contrib/instrgen` package to provide auto-generated source code instrumentation. (#3068, #3108)
 - The `go.opentelemetry.io/contrib/exporters/autoexport` package to provide configuration of trace exporters with useful defaults and envar support. (#2753, #4100)
+- `WithRouteTag` in `go.opentelemetry.io/contrib/instrumentation/net/http/otelhttp` adds HTTP route attribute to metrics. (#615)
 
 ### Fixed
 
diff --git a/instrumentation/net/http/otelhttp/handler.go b/instrumentation/net/http/otelhttp/handler.go
index f103aa627d6..123365caed1 100644
--- a/instrumentation/net/http/otelhttp/handler.go
+++ b/instrumentation/net/http/otelhttp/handler.go
@@ -258,12 +258,18 @@ func setAfterServeAttributes(span trace.Span, read, wrote int64, statusCode int,
 	span.SetAttributes(attributes...)
 }
 
-// WithRouteTag annotates a span with the provided route name using the
-// RouteKey Tag.
+// WithRouteTag annotates spans and metrics with the provided route name
+// with HTTP route attribute.
 func WithRouteTag(route string, h http.Handler) http.Handler {
 	return http.HandlerFunc(func(w http.ResponseWriter, r *http.Request) {
+		attr := semconv.HTTPRouteKey.String(route)
+
 		span := trace.SpanFromContext(r.Context())
-		span.SetAttributes(semconv.HTTPRoute(route))
+		span.SetAttributes(attr)
+
+		labeler, _ := LabelerFromContext(r.Context())
+		labeler.Add(attr)
+
 		h.ServeHTTP(w, r)
 	})
 }
diff --git a/instrumentation/net/http/otelhttp/test/handler_test.go b/instrumentation/net/http/otelhttp/test/handler_test.go
index a6e0ffd16f7..30eec741160 100644
--- a/instrumentation/net/http/otelhttp/test/handler_test.go
+++ b/instrumentation/net/http/otelhttp/test/handler_test.go
@@ -468,3 +468,70 @@ func TestSpanStatus(t *testing.T) {
 		})
 	}
 }
+
+func TestWithRouteTag(t *testing.T) {
+	route := "/some/route"
+
+	spanRecorder := tracetest.NewSpanRecorder()
+	tracerProvider := sdktrace.NewTracerProvider()
+	tracerProvider.RegisterSpanProcessor(spanRecorder)
+
+	metricReader := metric.NewManualReader()
+	meterProvider := metric.NewMeterProvider(metric.WithReader(metricReader))
+
+	h := otelhttp.NewHandler(
+		otelhttp.WithRouteTag(
+			route,
+			http.HandlerFunc(func(w http.ResponseWriter, r *http.Request) {
+				w.WriteHeader(http.StatusTeapot)
+			}),
+		),
+		"test_handler",
+		otelhttp.WithTracerProvider(tracerProvider),
+		otelhttp.WithMeterProvider(meterProvider),
+	)
+
+	h.ServeHTTP(httptest.NewRecorder(), httptest.NewRequest(http.MethodGet, "/", nil))
+	want := semconv.HTTPRouteKey.String(route)
+
+	require.Len(t, spanRecorder.Ended(), 1, "should emit a span")
+	gotSpan := spanRecorder.Ended()[0]
+	require.Contains(t, gotSpan.Attributes(), want, "should add route to span attributes")
+
+	rm := metricdata.ResourceMetrics{}
+	err := metricReader.Collect(context.Background(), &rm)
+	require.NoError(t, err)
+	require.Len(t, rm.ScopeMetrics, 1, "should emit metrics for one scope")
+	gotMetrics := rm.ScopeMetrics[0].Metrics
+
+	for _, m := range gotMetrics {
+		switch d := m.Data.(type) {
+		case metricdata.Sum[int64]:
+			require.Len(t, d.DataPoints, 1, "metric '%v' should have exactly one data point", m.Name)
+			require.Contains(t, d.DataPoints[0].Attributes.ToSlice(), want, "should add route to attributes for metric '%v'", m.Name)
+
+		case metricdata.Sum[float64]:
+			require.Len(t, d.DataPoints, 1, "metric '%v' should have exactly one data point", m.Name)
+			require.Contains(t, d.DataPoints[0].Attributes.ToSlice(), want, "should add route to attributes for metric '%v'", m.Name)
+
+		case metricdata.Histogram[int64]:
+			require.Len(t, d.DataPoints, 1, "metric '%v' should have exactly one data point", m.Name)
+			require.Contains(t, d.DataPoints[0].Attributes.ToSlice(), want, "should add route to attributes for metric '%v'", m.Name)
+
+		case metricdata.Histogram[float64]:
+			require.Len(t, d.DataPoints, 1, "metric '%v' should have exactly one data point", m.Name)
+			require.Contains(t, d.DataPoints[0].Attributes.ToSlice(), want, "should add route to attributes for metric '%v'", m.Name)
+
+		case metricdata.Gauge[int64]:
+			require.Len(t, d.DataPoints, 1, "metric '%v' should have exactly one data point", m.Name)
+			require.Contains(t, d.DataPoints[0].Attributes.ToSlice(), want, "should add route to attributes for metric '%v'", m.Name)
+
+		case metricdata.Gauge[float64]:
+			require.Len(t, d.DataPoints, 1, "metric '%v' should have exactly one data point", m.Name)
+			require.Contains(t, d.DataPoints[0].Attributes.ToSlice(), want, "should add route to attributes for metric '%v'", m.Name)
+
+		default:
+			require.Fail(t, "metric has unexpected data type", "metric '%v' has unexpected data type %T", m.Name, m.Data)
+		}
+	}
+}