diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8c18e2ca43..15c62f7077 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,6 +10,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Changed
 - `opentelemetry-instrumentation-botocore` Unpatch botocore Endpoint.prepare_request on uninstrument
   ([#664](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/664))
+- `opentelemetry-instrumentation-botocore` Fix span injection for lambda invoke
+  ([#663](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/663))
 
 ## [1.5.0-0.24b0](https://github.com/open-telemetry/opentelemetry-python/releases/tag/v1.5.0-0.24b0) - 2021-08-26
 
@@ -24,8 +26,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - `opentelemetry-instrumentation-fastapi` Allow instrumentation of newer FastAPI versions.
   ([#602](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/602))
-
-### Changed
 - Enable explicit `excluded_urls` argument in `opentelemetry-instrumentation-flask`
   ([#604](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/604))
 
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py b/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
index 634ba8b577..3212866b2e 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
@@ -150,15 +150,15 @@ def _patched_api_call(self, original_func, instance, args, kwargs):
         error = None
         result = None
 
-        # inject trace context into payload headers for lambda Invoke
-        if BotocoreInstrumentor._is_lambda_invoke(
-            service_name, operation_name, api_params
-        ):
-            BotocoreInstrumentor._patch_lambda_invoke(api_params)
-
         with self._tracer.start_as_current_span(
             "{}".format(service_name), kind=SpanKind.CLIENT,
         ) as span:
+            # inject trace context into payload headers for lambda Invoke
+            if BotocoreInstrumentor._is_lambda_invoke(
+                service_name, operation_name, api_params
+            ):
+                BotocoreInstrumentor._patch_lambda_invoke(api_params)
+
             if span.is_recording():
                 span.set_attribute("aws.operation", operation_name)
                 span.set_attribute("aws.region", instance.meta.region_name)
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py b/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
index 96ecac2b00..3c6a50251f 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
+++ b/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
@@ -435,11 +435,13 @@ def test_lambda_invoke_propagation(self):
 
             self.assertIn(MockTextMapPropagator.TRACE_ID_KEY, headers)
             self.assertEqual(
-                "0", headers[MockTextMapPropagator.TRACE_ID_KEY],
+                str(spans[2].get_span_context().trace_id),
+                headers[MockTextMapPropagator.TRACE_ID_KEY],
             )
             self.assertIn(MockTextMapPropagator.SPAN_ID_KEY, headers)
             self.assertEqual(
-                "0", headers[MockTextMapPropagator.SPAN_ID_KEY],
+                str(spans[2].get_span_context().span_id),
+                headers[MockTextMapPropagator.SPAN_ID_KEY],
             )
         finally:
             set_global_textmap(previous_propagator)