diff --git a/ci-operator/config/openshift/assisted-test-infra/openshift-assisted-test-infra-master.yaml b/ci-operator/config/openshift/assisted-test-infra/openshift-assisted-test-infra-master.yaml
index 7a85b1654385..57c205b7344e 100644
--- a/ci-operator/config/openshift/assisted-test-infra/openshift-assisted-test-infra-master.yaml
+++ b/ci-operator/config/openshift/assisted-test-infra/openshift-assisted-test-infra-master.yaml
@@ -687,16 +687,6 @@ tests:
         MAKEFILE_TARGET=test
       PLATFORM: vsphere
     workflow: assisted-vsphere
-- as: e2e-vsphere-assisted-periodic
-  cron: 00 05 */1 * 0-5
-  steps:
-    cluster_profile: vsphere-2
-    env:
-      ASSISTED_CONFIG: |
-        NUM_WORKERS=0
-        MAKEFILE_TARGET=test
-      PLATFORM: vsphere
-    workflow: assisted-vsphere
 - always_run: false
   as: e2e-vsphere-assisted-4-12
   optional: true
diff --git a/ci-operator/jobs/openshift/assisted-test-infra/openshift-assisted-test-infra-master-periodics.yaml b/ci-operator/jobs/openshift/assisted-test-infra/openshift-assisted-test-infra-master-periodics.yaml
index 05bf5b00c3f0..0faa17d8cbbb 100644
--- a/ci-operator/jobs/openshift/assisted-test-infra/openshift-assisted-test-infra-master-periodics.yaml
+++ b/ci-operator/jobs/openshift/assisted-test-infra/openshift-assisted-test-infra-master-periodics.yaml
@@ -1924,83 +1924,6 @@ periodics:
     - name: result-aggregator
       secret:
         secretName: result-aggregator
-- agent: kubernetes
-  cluster: vsphere02
-  cron: 00 05 */1 * 0-5
-  decorate: true
-  extra_refs:
-  - base_ref: master
-    org: openshift
-    repo: assisted-test-infra
-  labels:
-    ci-operator.openshift.io/cloud: vsphere
-    ci-operator.openshift.io/cloud-cluster-profile: vsphere-2
-    ci.openshift.io/generator: prowgen
-    job-release: "4.16"
-    pj-rehearse.openshift.io/can-be-rehearsed: "true"
-  name: periodic-ci-openshift-assisted-test-infra-master-e2e-vsphere-assisted-periodic
-  spec:
-    containers:
-    - args:
-      - --gcs-upload-secret=/secrets/gcs/service-account.json
-      - --image-import-pull-secret=/etc/pull-secret/.dockerconfigjson
-      - --lease-server-credentials-file=/etc/boskos/credentials
-      - --report-credentials-file=/etc/report/credentials
-      - --secret-dir=/secrets/ci-pull-credentials
-      - --secret-dir=/usr/local/e2e-vsphere-assisted-periodic-cluster-profile
-      - --target=e2e-vsphere-assisted-periodic
-      command:
-      - ci-operator
-      image: ci-operator:latest
-      imagePullPolicy: Always
-      name: ""
-      resources:
-        requests:
-          cpu: 10m
-      volumeMounts:
-      - mountPath: /etc/boskos
-        name: boskos
-        readOnly: true
-      - mountPath: /secrets/ci-pull-credentials
-        name: ci-pull-credentials
-        readOnly: true
-      - mountPath: /usr/local/e2e-vsphere-assisted-periodic-cluster-profile
-        name: cluster-profile
-      - mountPath: /secrets/gcs
-        name: gcs-credentials
-        readOnly: true
-      - mountPath: /secrets/manifest-tool
-        name: manifest-tool-local-pusher
-        readOnly: true
-      - mountPath: /etc/pull-secret
-        name: pull-secret
-        readOnly: true
-      - mountPath: /etc/report
-        name: result-aggregator
-        readOnly: true
-    serviceAccountName: ci-operator
-    volumes:
-    - name: boskos
-      secret:
-        items:
-        - key: credentials
-          path: credentials
-        secretName: boskos-credentials
-    - name: ci-pull-credentials
-      secret:
-        secretName: ci-pull-credentials
-    - name: cluster-profile
-      secret:
-        secretName: cluster-secrets-vsphere
-    - name: manifest-tool-local-pusher
-      secret:
-        secretName: manifest-tool-local-pusher
-    - name: pull-secret
-      secret:
-        secretName: registry-pull-credentials
-    - name: result-aggregator
-      secret:
-        secretName: result-aggregator
 - agent: kubernetes
   cluster: build03
   cron: '*/30 * * * *'