ncbi_blastx_wrapper.xml

<tool id="ncbi_blastx_wrapper" name="NCBI BLAST+ blastx" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
    <description>Search protein database with translated nucleotide query sequence(s)</description>
    <macros>
        <token name="@BINARY@">blastx</token>
        <import>ncbi_macros.xml</import>
    </macros>
    <expand macro="parallelism" />
    <expand macro="preamble" />
    <command detect_errors="aggressive">
## The command is a Cheetah template which allows some Python based syntax.
## Lines starting hash hash are comments. Galaxy will turn newlines into spaces
blastx
@QUERY@
@BLAST_DB_SUBJECT@
-query_gencode $query_gencode
-task $blast_type
-evalue $evalue_cutoff
@BLAST_OUTPUT@
@THREADS@
#if $adv_opts.adv_opts_selector=="advanced":
$adv_opts.strand
@ADV_MATRIX_GAPCOSTS@
@ADV_FILTER_QUERY@
@ADV_MAX_HITS@
@ADV_WORD_SIZE@
$adv_opts.ungapped
@ADV_ID_LIST_FILTER@
@ADV_QCOV_HSP_PERC@
@ADV_WINDOW_SIZE@
@ADV_THRESHOLD@
@ADV_COMP_BASED_STATS@
## End of advanced options:
#end if
    </command>
    <inputs>
        <expand macro="nucl_query" />

        <expand macro="input_conditional_protein_db" />
        <expand macro="input_query_gencode" />
        <param name="blast_type" argument="-task" type="select" display="radio" label="Type of BLAST">
            <option value="blastx">blastx - Traditional BLASTX to compare translated nucleotide query to protein database</option>
            <option value="blastx-fast">blastx-fast - Use longer words for seeding, faster but less accurate</option>
        </param>
        <expand macro="input_evalue" />

        <expand macro="input_out_format" />
        <expand macro="advanced_options">
            <!-- Could use a select (yes, no, other) where other allows setting 'window locut hicut' -->
            <expand macro="input_filter_query_default_true" />
            <expand macro="input_strand" />
            <expand macro="input_matrix_gapcosts" />
            <expand macro="input_max_hits" />
            <expand macro="input_word_size" />
            <param argument="-ungapped" type="boolean" label="Perform ungapped alignment only?" truevalue="-ungapped" falsevalue="" checked="false" />
            <expand macro="input_parse_deflines" />
            <expand macro="advanced_optional_id_files" />
            <expand macro="input_qcov_hsp_perc" />
            <expand macro="input_window_size" />
            <expand macro="input_threshold" />
            <expand macro="input_comp_based_stats" />
        </expand>
    </inputs>
    <outputs>
        <data name="output1" format="tabular" label="blastx $query.name vs @ON_DB_SUBJECT@">
            <expand macro="output_change_format" />
        </data>
    </outputs>
    <tests>
        <test>
            <param name="query" value="rhodopsin_nucs.fasta" ftype="fasta" />
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="four_human_proteins.fasta" ftype="fasta" />
            <param name="database" value="" />
            <param name="evalue_cutoff" value="1e-10" />
            <param name="out_format" value="5" />
            <param name="adv_opts_selector" value="basic" />
            <output name="output1" file="blastx_rhodopsin_vs_four_human.xml" ftype="blastxml" />
        </test>
        <test>
            <param name="query" value="rhodopsin_nucs.fasta" ftype="fasta" />
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="four_human_proteins.fasta" ftype="fasta" />
            <param name="database" value="" />
            <param name="evalue_cutoff" value="1e-10" />
            <param name="out_format" value="6" />
            <param name="adv_opts_selector" value="basic" />
            <output name="output1" file="blastx_rhodopsin_vs_four_human.tabular" ftype="tabular" />
        </test>
        <test>
            <param name="query" value="rhodopsin_nucs.fasta" ftype="fasta" />
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="four_human_proteins.fasta" ftype="fasta" />
            <param name="database" value="" />
            <param name="evalue_cutoff" value="1e-10" />
            <param name="out_format" value="ext" />
            <param name="adv_opts_selector" value="basic" />
            <output name="output1" file="blastx_rhodopsin_vs_four_human_ext.tabular" ftype="tabular" />
        </test>
        <test>
            <param name="query" value="rhodopsin_nucs.fasta" ftype="fasta" />
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="four_human_proteins.fasta" ftype="fasta" />
            <param name="database" value="" />
            <param name="evalue_cutoff" value="1e-10" />
            <param name="out_format" value="cols" />
            <param name="std_cols" value="qseqid,sseqid,pident,length,mismatch,gapopen,qstart,qend,sstart,send,evalue,bitscore" />
            <param name="ext_cols" value="sallseqid,score,nident,positive,gaps,ppos,qframe,sframe,qseq,sseq,qlen,slen,salltitles" />
            <param name="ids_cols" value="qgi,qacc,qaccver,sallseqid,sgi,sallgi,sacc,saccver,sallacc,stitle" />
            <param name="misc_cols" value="sstrand,frames,btop,qcovs,qcovhsp" />
            <param name="tax_cols" value="staxids,sscinames,scomnames,sblastnames,sskingdoms" />
            <param name="adv_opts_selector" value="basic" />
            <output name="output1" file="blastx_rhodopsin_vs_four_human_all.tabular" ftype="tabular" />
        </test>
        <test>
            <param name="query" value="rhodopsin_nucs.fasta" ftype="fasta" />
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="four_human_proteins.fasta" ftype="fasta" />
            <param name="database" value="" />
            <param name="evalue_cutoff" value="1e-10" />
            <param name="out_format" value="6" />
            <param name="adv_opts_selector" value="advanced" />
            <param name="matrix" value="BLOSUM62" />
            <output name="output1" file="blastx_rhodopsin_adv_vs_four_human.tabular" ftype="tabular" />
        </test>
    </tests>
    <help>

@SEARCH_TIME_WARNING@

**What it does**

Search a *protein database* using a *translated nucleotide query*,
using the NCBI BLAST+ blastx command line tool.

@FASTA_WARNING@

-------

@OUTPUT_FORMAT@

-------

@CLI_OPTIONS@

-------

**References**

If you use this Galaxy tool in work leading to a scientific publication please
cite the following papers:

@REFERENCES@
    </help>
    <expand macro="blast_citations" />
</tool>