It places contigs!
What does it do?
It compares unplaced contigs to SNPs/genotypes on placed contigs and places unplaced contigs next to their best partner using a penalised Hamming distance. In a way, it's similar to genetic mapping algorithms - but genetic mapping algorithms make de novo maps, I just wanted to place some newly assembled contigs into an already existing assembly.
It takes genotyping data as input.
What does it do, in detail?
For each unplaced contig, it collects all SNPs located on that SNPs and merges them into one "metaSNP" which for each individual carries the most common allele. Recombinations on a contig are rare, so in many cases the genotypes for one individual on one contig are identical. It also merges all SNPs for placed contigs.
Then, it compares all unplaced contigs against all placed contigs with a penalised Hamming distance - if both alleles for the individual are missing, add 0.75, if one is missing, add 0.5, if they are different, add 1, if they are identical, add 0. That means that the contig placed with the lowest score is the "best" score, preferably 0.
Then it does a few extra tricks to increase accuracy:
Are the best and the second-best placed partner contig on different chromosomes? If so, discard the contig, it's not placeable.
Can we orient the contig? Take the first and last 5 SNPs if there are more or equal to 10 SNPs located on it, and see whether the last 5 SNPs are more similar to the SNPs of the preceding contig, and whether the first 5 SNPs are closer to the succeeding contig. If that is the case, reverse complement the contig. (Note: This happens only rarely in my tests).
It then places each unplaced contig behind its best partner contig. If several unplaced contig are placed behind the same placed contig, they are ordered based on how similar the unplaced contigs are to each other.
How long does it run?
With a large plant genome and about a million SNPs it takes a few hours using one CPU on my laptop.
How accurate is it?
Probably not that accurate - some (unpublished) tests show that the chromosome placement is ~ 98% correct, but I cannot test the base-pair level placement without optical maps etc. I would assume that the base-pair level placement is not very accurate. As in genetic mapping algorithms, contigs that have identical genotypes are indistinguishable.
What does it take as input files?
For each collection of unplaced contigs, and for each pseudo-molecule assembly, contigPlacer takes three input files.
Since I work mostly with Flapjack, I used the input map and dat file from Flapjack, and a gff3 file detailing where each contig is. A simple example:
The map file (tab-delimited), one SNP per line, SNP-ID, chromosome, position:
#fjFile = MAP M1 A01 10 M2 A01 20 M1000 A01 9,292 etc.
The dat file (tab-delimited), one individual per line, first the header with all SNP-IDs, then for each line individual-ID, and all genotypes:
#fjFile = GENOTYPE M1 M2 M1000 etc. Ind1 A A B etc. Ind2 B B A etc.
The gff3 file detailing contigs, one contig per line, only the 4th, 5th and 9th element are important:
##gff-version 3 ##sequence-region A01 1 29136790 A01 fasta contig 1 4521 . . . ID=Contig_1;Name=Contig_1 A01 fasta contig 4522 8999 . . . ID=Contig_2;Name=Contig_2 etc.
The paths to these control files are detailed in two control files - one file for the unplaced contigs, and one file for the placed contigs in pseudo-chromosomes, always in the order dat, map and gff3 (tab-delimited)
Placed contigs file:
placed_A01.dat placed_A01.map placed_A01.gff3 placed_A02.dat placed_A02.map placed_A02.gff3 etc.
And the same for the unplaced contigs file. Relative or absolute paths are fine.
All you need is to install Go from https://golang.org/
I've tested it with a few versions from 1.2 to 1.5.1. I've tried to keep it free from external dependencies as that just makes things complicated.
I've only tested it under Fedora and Ubuntu Linux, no clue whether it works under Windows.
go build in this directory to get the compiled contigPlacer. As it's a self-contained binary you can deploy the resulting file to your server.
go run contigPlacer.Go
or (after building)
with these flags:
-chrom chromFile -contig toPlaceFile
toPlaceFile are files detailing paths to the placed and unplaced contigs - see above. The path can be relative or absolute.
This does not place unplaced contigs when their best partner score is above a certain threshold - I'd use 1/3 * the size of the population you have. I STRONGLY recommend you to use this flag.
-comparison hamming (default), percentage, r_squared
This changes the comparison function. By default, it uses the Hamming distance. A few tests show this option not to make a big difference - since Hamming is penalising missing alleles I'd assume you place less in unimputed datasets compared to simple
You get one gff3 file per collection of placed contigs detailing where the original contigs are located, and where the new contigs are placed. You can use
makeChromosomesFromContigplacer.py to make fasta files out of these files.
You also get one file called
all_scores.txt which details the best score (Hamming distance - 0 means no difference between the unplaced contig and the best partner-contig) for each unplaced contig. This file also stores for each unplaced contig how many recombinations per individual were counted on the contig.
You also get
list_of_unplaceable_contigs.txt, which details for what reason which contig couldn't be placed. This can be "No_SNPs", "Different_partners" (first and second best partner of an unplaced contig are on different chromosomes), and Score_too_low when you've supplied a cutoff.
It's licensed under the MIT license. If you work for a commercial entity and want to use this software, I would like to request you to contact me via email (firstname.lastname@example.org), our group (the Applied Bioinformatics Group at the University of Western Australia) is always looking for partners in ARC linkage grants.