diff --git a/pre_commit/languages/julia.py b/pre_commit/languages/julia.py
index df91c0697..7559b5ba6 100644
--- a/pre_commit/languages/julia.py
+++ b/pre_commit/languages/julia.py
@@ -37,7 +37,7 @@ def run_hook(
 
     cmd = lang_base.hook_cmd(entry, args)
     script = cmd[0] if is_local else prefix.path(cmd[0])
-    cmd = ('julia', script, *cmd[1:])
+    cmd = ('julia', '--startup-file=no', script, *cmd[1:])
     return lang_base.run_xargs(
         cmd,
         file_args,
@@ -127,6 +127,7 @@ def install_environment(
         end
         """
         cmd_output_b(
-            'julia', '-e', julia_code, '--', envdir, *additional_dependencies,
+            'julia', '--startup-file=no', '-e', julia_code, '--', envdir,
+            *additional_dependencies,
             cwd=prefix.prefix_dir,
         )
diff --git a/tests/languages/julia_test.py b/tests/languages/julia_test.py
index 4ea3c25b2..175622d65 100644
--- a/tests/languages/julia_test.py
+++ b/tests/languages/julia_test.py
@@ -1,5 +1,8 @@
 from __future__ import annotations
 
+import os
+from unittest import mock
+
 from pre_commit.languages import julia
 from testing.language_helpers import run_language
 from testing.util import cwd
@@ -28,6 +31,17 @@ def test_julia_hook(tmp_path):
     assert run_language(tmp_path, julia, 'src/main.jl') == expected
 
 
+def test_julia_hook_with_startup(tmp_path):
+    depot_path = tmp_path.joinpath('depot')
+    depot_path.joinpath('config').mkdir(parents=True)
+    startup = depot_path.joinpath('config', 'startup.jl')
+    startup.write_text('error("Startup file used!")\n')
+
+    depo_path_var = f'{depot_path}{os.pathsep}'
+    with mock.patch.dict(os.environ, {'JULIA_DEPOT_PATH': depo_path_var}):
+        test_julia_hook(tmp_path)
+
+
 def test_julia_hook_manifest(tmp_path):
     code = """
     using Example