Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).
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Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl.


sudo apt-get install -y build-essential g++ cmake git-all

git clone primer3

cd primer3/src


make test

Run Primer3

./primer3_core ../example

Read the complete Primer3 manual

Primer3 Manual

or see /src/primer3_manual.htm