Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).
Use Git or checkout with SVN using the web URL.
Work fast with our official CLI. Learn more.
Please sign in to use Codespaces.
If nothing happens, download GitHub Desktop and try again.
If nothing happens, download GitHub Desktop and try again.
If nothing happens, download Xcode and try again.
Your codespace will open once ready.
There was a problem preparing your codespace, please try again.
Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl.
sudo apt-get install -y build-essential g++ cmake git-all
git clone https://github.com/primer3-org/primer3.git primer3
cd primer3/src
make
make test
./primer3_core ../example
or see /src/primer3_manual.htm
Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).