SEQUENCE_TARGET=200,300
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_NUM_RETURN=5
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_PRODUCT_SIZE_RANGE=500-800
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/home/lhs/softwares/primer3-master/src/primer3_config/
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1
PRIMER_MAX_SELF_ANY_TH=20.00
PRIMER_PAIR_MAX_COMPL_ANY_TH=20.00
PRIMER_MAX_SELF_END_TH=20.00
PRIMER_PAIR_MAX_COMPL_END_TH=20.00
PRIMER_MAX_HAIRPIN_TH=20.00
PRIMER_OUTSIDE_PENALTY=-1.0
PRIMER_EXPLAIN_FLAG=1
P3_FILE_FLAG=1
=
SEQUENCE_ID=test1
SEQUENCE_TEMPLATE=ACCCTATTTCCACCTATCCAAAATGGAGAAAGTTCATGTTGACTTAGACGCAGACAGCCCATTCGTCAAGTCACTGCAAAGATGCTTTCCACATTTTGAGATAGAAGCAACGCAGGTCACTGACAATGACCATGCTAATGCTAGGGCGTTTTCGCACCTAGCTACTAAGCTCATTGAGGGAGAAGTGGATACAGACCAGGTGATCCTGGATATCGGGAGCGCGCCTGTAAGGCACACGCATTCCAAACATAAGTACCACTGCATTTGCCCAATGAAGAGCGCAGAAGACCCTGACAGACTCTACCGCTACGCAGACAAGCTTAGAAAGAGTGATGTCACTGACAAATGTATTGCCTCTAAGGCCGCGGACCTGCTAACAGTAATGTCGACGCCTGACGCTGAGACACCCTCGTTATGCATGCACACTGACTCAACTTGCAGGTACCACGGCTCCGTGGCCGTATATCAGGATGTATATGCAGTGCATGCACCGACTTCCATTTACTACCAGGCGCTGAAAGGTGTACGAACTATCTATTGGATCGGGTTCGATACTACACCGTTCATGTATAAGAACATGGCAGGCGCCTACCCTACATACAACACTAATTGGGCCGATGAAAGTGTGTTGGAAGCCAGAAATATAGGGCTGGGTAGTTCAGACTTGCACGAAAAGAGTTTCGGAAAAGTATCCATTATGAGGAAGAAGAAATTACAACCCACCAATAAAGTAATATTTTCTGTGGGGTCAACTATTTATACTGAAGAGAGAATACTGTTACGCAGTTGGCATCTACCTAATGTTTTTCATCTAAAAGGTAAAACTAGCTTTACAGGCAGATGTAACACTATCGTCAGCTGCGAAGGTTACGTTGTCAAGAAGATTACGCTCAGTCCTGGGATTTACGGGAAAGTGGATAATCTTGCTTCGACCATGCACCGAGAGGGATTCTTAAGTTGCAAGGTTACAGATACGTTAAGAGGGGAGAGGGTCTCTTTT
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