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Receptors and sequences

Receptors pages

This video demonstrates how to use the receptor pages.

  • By moving the pointer over residues in the sequence viewer, more information can be displayed.
  • Snake and Helix box plots can be custom colored and downloaded.
  • A summary of available mutant data is displayed, and a link provided for further analysis.
  • Available structure information is listed.
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Questions

  • Look up the receptor page for the beta1-adrenoceptor. How many mutations are there available?
  • Look up the receptor page for your favorite GPCR. Is there any data available?

Structure-based sequence alignments

This video demonstrates how to build a structure-based sequence alignment.

  • Any combination of receptors and/or receptor families can be selected.
  • It is possible to limit the alignment to a particular part of the sequence, e.g. TM6.
  • By moving the pointer over residues in the alignment, more information can be displayed.
  • A consensus sequence (color coded by conservation) is displayed below the alignment.
  • Statistics on residue and residue property conservation is displayed below the consensus sequence.
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Question

  • Create an alignment of TM5 for all Class A peptide receptors. How well conserved is position 5.50?

Similarity search

This video demonstrates how to perform similarity search with a reference receptor.

  • Only one receptor can be selected as reference, and the other selected receptors will be compared to the reference.
  • It is possible to limit the search to a particular part of the sequence, e.g. TM6.
  • In the resulting sequence alignment, the sequences are ranked by similarity to the reference, and their similarity scores are shown to the right of the receptor name.
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Question

  • Look up the similarity of the FFA1 receptor against Class A lipid receptors using the 7TMs. Which receptor is most similar?