diff --git a/dependencies/default/requirements.txt b/dependencies/default/requirements.txt
index 01b2484e..a0009a85 100644
--- a/dependencies/default/requirements.txt
+++ b/dependencies/default/requirements.txt
@@ -1,4 +1,4 @@
 # Always adjust install_requires in setup.cfg and pytest-min-requirements.txt
 # when changing runtime dependencies
-pytest >= 6.1.0
+pytest >= 7.0.0
 typing-extensions >= 3.7.2; python_version < "3.8"
diff --git a/dependencies/pytest-min/constraints.txt b/dependencies/pytest-min/constraints.txt
index 33f7948f..1f82dbaf 100644
--- a/dependencies/pytest-min/constraints.txt
+++ b/dependencies/pytest-min/constraints.txt
@@ -1,22 +1,22 @@
-async-generator==1.10
-attrs==21.4.0
-coverage==6.3.2
-flaky==3.7.0
-hypothesis==6.43.3
-idna==3.3
+argcomplete==2.0.0
+attrs==22.1.0
+certifi==2022.9.24
+charset-normalizer==2.1.1
+elementpath==3.0.2
+exceptiongroup==1.0.0rc9
+hypothesis==6.56.3
+idna==3.4
 iniconfig==1.1.1
-mypy==0.942
-mypy-extensions==0.4.3
-outcome==1.1.0
+mock==4.0.3
+nose==1.3.7
 packaging==21.3
-pluggy==0.13.1
+pluggy==1.0.0
 py==1.11.0
-pyparsing==3.0.8
-pytest==6.1.0
-pytest-trio==0.7.0
-sniffio==1.2.0
+Pygments==2.13.0
+pyparsing==3.0.9
+pytest==7.0.0
+requests==2.28.1
 sortedcontainers==2.4.0
-toml==0.10.2
 tomli==2.0.1
-trio==0.20.0
-typing_extensions==4.2.0
+urllib3==1.26.12
+xmlschema==2.1.1
diff --git a/dependencies/pytest-min/requirements.txt b/dependencies/pytest-min/requirements.txt
index 4fc6ef2f..4152d2f8 100644
--- a/dependencies/pytest-min/requirements.txt
+++ b/dependencies/pytest-min/requirements.txt
@@ -1,4 +1,4 @@
 # Always adjust install_requires in setup.cfg and requirements.txt
 # when changing minimum version dependencies
-pytest == 6.1.0
+pytest[testing] == 7.0.0
 typing-extensions >= 3.7.2; python_version < "3.8"
diff --git a/docs/source/reference/changelog.rst b/docs/source/reference/changelog.rst
index e6f80383..af4eb7bf 100644
--- a/docs/source/reference/changelog.rst
+++ b/docs/source/reference/changelog.rst
@@ -2,6 +2,10 @@
 Changelog
 =========
 
+UNRELEASED
+=================
+- Drop compatibility with pytest 6.1. Pytest-asyncio now depends on pytest 7.0 or newer.
+
 0.20.3 (22-12-08)
 =================
 - Prevent DeprecationWarning to bubble up on CPython 3.10.9 and 3.11.1.
diff --git a/pytest_asyncio/plugin.py b/pytest_asyncio/plugin.py
index 403d814f..f663ff68 100644
--- a/pytest_asyncio/plugin.py
+++ b/pytest_asyncio/plugin.py
@@ -25,7 +25,15 @@
 )
 
 import pytest
-from pytest import Function, Item, Session
+from pytest import (
+    Config,
+    FixtureRequest,
+    Function,
+    Item,
+    Parser,
+    PytestPluginManager,
+    Session,
+)
 
 if sys.version_info >= (3, 8):
     from typing import Literal
@@ -46,11 +54,9 @@
 FixtureFunction = Union[SimpleFixtureFunction, FactoryFixtureFunction]
 FixtureFunctionMarker = Callable[[FixtureFunction], FixtureFunction]
 
-Config = Any  # pytest < 7.0
-PytestPluginManager = Any  # pytest < 7.0
-FixtureDef = Any  # pytest < 7.0
-Parser = Any  # pytest < 7.0
-SubRequest = Any  # pytest < 7.0
+# https://github.com/pytest-dev/pytest/pull/9510
+FixtureDef = Any
+SubRequest = Any
 
 
 class Mode(str, enum.Enum):
@@ -169,14 +175,6 @@ def pytest_configure(config: Config) -> None:
         "run using an asyncio event loop",
     )
 
-    if getattr(pytest, "version_tuple", (0, 0, 0)) < (7,):
-        warnings.warn(
-            "You're using an outdated version of pytest. Newer releases of "
-            "pytest-asyncio will not be compatible with this pytest version. "
-            "Please update pytest to version 7 or later.",
-            DeprecationWarning,
-        )
-
 
 @pytest.hookimpl(tryfirst=True)
 def pytest_report_header(config: Config) -> List[str]:
@@ -508,7 +506,7 @@ def pytest_runtest_setup(item: pytest.Item) -> None:
 
 
 @pytest.fixture
-def event_loop(request: "pytest.FixtureRequest") -> Iterator[asyncio.AbstractEventLoop]:
+def event_loop(request: FixtureRequest) -> Iterator[asyncio.AbstractEventLoop]:
     """Create an instance of the default event loop for each test case."""
     loop = asyncio.get_event_loop_policy().new_event_loop()
     yield loop
diff --git a/setup.cfg b/setup.cfg
index a82c57e1..ee57438c 100644
--- a/setup.cfg
+++ b/setup.cfg
@@ -40,7 +40,7 @@ include_package_data = True
 
 # Always adjust requirements.txt and pytest-min-requirements.txt when changing runtime dependencies
 install_requires =
-  pytest >= 6.1.0
+  pytest >= 7.0.0
   typing-extensions >= 3.7.2; python_version < "3.8"
 
 [options.extras_require]
diff --git a/tests/test_pytest_min_version_warning.py b/tests/test_pytest_min_version_warning.py
deleted file mode 100644
index 5f7bd72f..00000000
--- a/tests/test_pytest_min_version_warning.py
+++ /dev/null
@@ -1,26 +0,0 @@
-from textwrap import dedent
-
-import pytest
-
-
-@pytest.mark.skipif(
-    pytest.__version__ < "7.0.0",
-    reason="The warning shouldn't be present when run with recent pytest versions",
-)
-@pytest.mark.parametrize("mode", ("auto", "strict"))
-def test_pytest_min_version_warning_is_not_triggered_for_pytest_7(testdir, mode):
-    testdir.makepyfile(
-        dedent(
-            """\
-            import pytest
-
-            pytest_plugins = 'pytest_asyncio'
-
-            @pytest.mark.asyncio
-            async def test_triggers_pytest_warning():
-                pass
-            """
-        )
-    )
-    result = testdir.runpytest(f"--asyncio-mode={mode}")
-    result.assert_outcomes(passed=1, warnings=0)