diff --git a/src/poetry/core/factory.py b/src/poetry/core/factory.py
index 98e44631b..de4ae8a4b 100644
--- a/src/poetry/core/factory.py
+++ b/src/poetry/core/factory.py
@@ -286,6 +286,7 @@ def create_dependency(
                 dependency = FileDependency(
                     name,
                     file_path,
+                    directory=constraint.get("subdirectory", None),
                     groups=groups,
                     base=root_dir,
                     extras=constraint.get("extras", []),
@@ -302,12 +303,16 @@ def create_dependency(
                     dependency = FileDependency(
                         name,
                         path,
+                        directory=constraint.get("subdirectory", None),
                         groups=groups,
                         optional=optional,
                         base=root_dir,
                         extras=constraint.get("extras", []),
                     )
                 else:
+                    subdirectory = constraint.get("subdirectory", None)
+                    if subdirectory:
+                        path = path / subdirectory
                     dependency = DirectoryDependency(
                         name,
                         path,
diff --git a/src/poetry/core/json/schemas/poetry-schema.json b/src/poetry/core/json/schemas/poetry-schema.json
index 790367fe2..7eaffe9b8 100644
--- a/src/poetry/core/json/schemas/poetry-schema.json
+++ b/src/poetry/core/json/schemas/poetry-schema.json
@@ -428,6 +428,10 @@
           "type": "string",
           "description": "The path to the file."
         },
+        "subdirectory": {
+          "type": "string",
+          "description": "The relative path to the directory where the package is located."
+        },
         "python": {
           "type": "string",
           "description": "The python versions for which the dependency should be installed."
@@ -464,6 +468,10 @@
           "type": "string",
           "description": "The path to the dependency."
         },
+        "subdirectory": {
+          "type": "string",
+          "description": "The relative path to the directory where the package is located."
+        },
         "python": {
           "type": "string",
           "description": "The python versions for which the dependency should be installed."
diff --git a/src/poetry/core/packages/dependency.py b/src/poetry/core/packages/dependency.py
index 6f97f2ad0..9706c511c 100644
--- a/src/poetry/core/packages/dependency.py
+++ b/src/poetry/core/packages/dependency.py
@@ -447,7 +447,11 @@ def create_from_pep_508(
                 # handle RFC 8089 references
                 path = url_to_path(req.url)
                 dep = _make_file_or_dir_dep(
-                    name=name, path=path, base=relative_to, extras=req.extras
+                    name=name,
+                    path=path,
+                    base=relative_to,
+                    subdirectory=link.subdirectory_fragment,
+                    extras=req.extras,
                 )
             else:
                 with suppress(ValueError):
@@ -511,6 +515,7 @@ def _make_file_or_dir_dep(
     name: str,
     path: Path,
     base: Path | None = None,
+    subdirectory: str | None = None,
     extras: list[str] | None = None,
 ) -> FileDependency | DirectoryDependency | None:
     """
@@ -526,7 +531,9 @@ def _make_file_or_dir_dep(
         _path = Path(base) / path
 
     if _path.is_file():
-        return FileDependency(name, path, base=base, extras=extras)
+        return FileDependency(
+            name, path, base=base, directory=subdirectory, extras=extras
+        )
     elif _path.is_dir():
         return DirectoryDependency(name, path, base=base, extras=extras)
 
diff --git a/src/poetry/core/packages/file_dependency.py b/src/poetry/core/packages/file_dependency.py
index a5dade981..0835ab1c6 100644
--- a/src/poetry/core/packages/file_dependency.py
+++ b/src/poetry/core/packages/file_dependency.py
@@ -15,6 +15,8 @@ def __init__(
         self,
         name: str,
         path: Path,
+        *,
+        directory: str | None = None,
         groups: Iterable[str] | None = None,
         optional: bool = False,
         base: Path | None = None,
@@ -23,6 +25,7 @@ def __init__(
         self._path = path
         self._base = base or Path.cwd()
         self._full_path = path
+        self._directory = directory
 
         if not self._path.is_absolute():
             try:
@@ -44,6 +47,7 @@ def __init__(
             allows_prereleases=True,
             source_type="file",
             source_url=self._full_path.as_posix(),
+            source_subdirectory=directory,
             extras=extras,
         )
 
@@ -59,6 +63,10 @@ def path(self) -> Path:
     def full_path(self) -> Path:
         return self._full_path
 
+    @property
+    def directory(self) -> str | None:
+        return self._directory
+
     def is_file(self) -> bool:
         return True
 
@@ -83,4 +91,7 @@ def base_pep_508_name(self) -> str:
         )
         requirement += f" @ {path}"
 
+        if self.directory:
+            requirement += f"#subdirectory={self.directory}"
+
         return requirement
diff --git a/src/poetry/core/packages/package.py b/src/poetry/core/packages/package.py
index 9c87eda9a..cf36b30f8 100644
--- a/src/poetry/core/packages/package.py
+++ b/src/poetry/core/packages/package.py
@@ -495,6 +495,7 @@ def to_dependency(self) -> Dependency:
             dep = FileDependency(
                 self._name,
                 Path(cast(str, self._source_url)),
+                directory=self.source_subdirectory,
                 groups=list(self._dependency_groups.keys()),
                 optional=self.optional,
                 base=self.root_dir,
diff --git a/tests/fixtures/project_with_dependencies_with_subdirectory/README.rst b/tests/fixtures/project_with_dependencies_with_subdirectory/README.rst
new file mode 100644
index 000000000..f7fe15470
--- /dev/null
+++ b/tests/fixtures/project_with_dependencies_with_subdirectory/README.rst
@@ -0,0 +1,2 @@
+My Package
+==========
diff --git a/tests/fixtures/project_with_dependencies_with_subdirectory/pyproject.toml b/tests/fixtures/project_with_dependencies_with_subdirectory/pyproject.toml
new file mode 100644
index 000000000..661936083
--- /dev/null
+++ b/tests/fixtures/project_with_dependencies_with_subdirectory/pyproject.toml
@@ -0,0 +1,40 @@
+[tool.poetry]
+name = "my-package"
+version = "1.2.3"
+description = "Some description."
+authors = [
+    "Sébastien Eustace <sebastien@eustace.io>"
+]
+license = "MIT"
+
+readme = "README.rst"
+
+homepage = "https://python-poetry.org"
+repository = "https://github.com/python-poetry/poetry"
+documentation = "https://python-poetry.org/docs"
+
+keywords = ["packaging", "dependency", "poetry"]
+
+classifiers = [
+    "Topic :: Software Development :: Build Tools",
+    "Topic :: Software Development :: Libraries :: Python Modules"
+]
+
+# Requirements
+[tool.poetry.dependencies]
+python = "~2.7 || ^3.6"
+
+# Git dependency with subdirectory
+pendulum = { git = "https://github.com/sdispater/pendulum.git", subdirectory = "sub", branch = "2.0" }
+
+# File dependency with subdirectory
+demo = [
+    { path = "../distributions/demo-0.1.0-py2.py3-none-any.whl", subdirectory = "sub", platform = "linux" },
+    { file = "../distributions/demo-0.1.0-py2.py3-none-any.whl", subdirectory = "sub", platform = "win32" }
+]
+
+# Dir dependency with subdirectory (same as path "../simple_project" without subdirectory)
+simple-project = { path = "..", subdirectory = "simple_project" }
+
+# Url dependency with subdirectory
+foo = { url = "https://example.com/foo.zip", subdirectory = "sub" }
diff --git a/tests/packages/test_file_dependency.py b/tests/packages/test_file_dependency.py
index ecbf06fb7..97ac3326f 100644
--- a/tests/packages/test_file_dependency.py
+++ b/tests/packages/test_file_dependency.py
@@ -149,7 +149,15 @@ def test_file_dependency_pep_508_local_file_relative_path(
     _test_file_dependency_pep_508(mocker, "demo", path, requirement, expected)
 
 
-def test_absolute_file_dependency_to_pep_508_with_marker(mocker: MockerFixture) -> None:
+def test_file_dependency_pep_508_with_subdirectory(mocker: MockerFixture) -> None:
+    path = DIST_PATH / "demo.zip"
+    expected = f"demo @ {path.as_uri()}#subdirectory=sub"
+
+    requirement = f"demo @ file://{path.as_posix()}#subdirectory=sub"
+    _test_file_dependency_pep_508(mocker, "demo", path, requirement, expected)
+
+
+def test_to_pep_508_with_marker(mocker: MockerFixture) -> None:
     wheel = "demo-0.1.0-py2.py3-none-any.whl"
 
     abs_path = DIST_PATH / wheel
diff --git a/tests/packages/test_package.py b/tests/packages/test_package.py
index 610870a57..19e31c1c8 100644
--- a/tests/packages/test_package.py
+++ b/tests/packages/test_package.py
@@ -365,6 +365,7 @@ def test_to_dependency_for_file() -> None:
         "1.2.3",
         source_type="file",
         source_url=path.as_posix(),
+        source_subdirectory="qux",
         features=["baz", "bar"],
     )
     dep = package.to_dependency()
@@ -377,6 +378,7 @@ def test_to_dependency_for_file() -> None:
     assert dep.path == path
     assert dep.source_type == "file"
     assert dep.source_url == path.as_posix()
+    assert dep.source_subdirectory == "qux"
 
 
 def test_to_dependency_for_url() -> None:
diff --git a/tests/test_factory.py b/tests/test_factory.py
index 3849d4d4d..61ee1410f 100644
--- a/tests/test_factory.py
+++ b/tests/test_factory.py
@@ -8,6 +8,8 @@
 import pytest
 
 from poetry.core.factory import Factory
+from poetry.core.packages.directory_dependency import DirectoryDependency
+from poetry.core.packages.file_dependency import FileDependency
 from poetry.core.packages.url_dependency import URLDependency
 from poetry.core.packages.vcs_dependency import VCSDependency
 from poetry.core.semver.helpers import parse_constraint
@@ -146,6 +148,54 @@ def test_create_poetry() -> None:
     ]
 
 
+def test_create_poetry_with_dependencies_with_subdirectory() -> None:
+    poetry = Factory().create_poetry(
+        fixtures_dir / "project_with_dependencies_with_subdirectory"
+    )
+    package = poetry.package
+    dependencies = {str(dep.name): dep for dep in package.requires}
+
+    # git dependency
+    pendulum = dependencies["pendulum"]
+    assert pendulum.is_vcs()
+    assert pendulum.pretty_constraint == "branch 2.0"
+    pendulum = cast(VCSDependency, pendulum)
+    assert pendulum.source == "https://github.com/sdispater/pendulum.git"
+    assert pendulum.directory == "sub"
+
+    # file dependency
+    demo = dependencies["demo"]
+    assert demo.is_file()
+    assert demo.pretty_constraint == "*"
+    demo = cast(FileDependency, demo)
+    assert demo.path == Path("../distributions/demo-0.1.0-py2.py3-none-any.whl")
+    assert demo.directory == "sub"
+    demo_dependencies = [dep for dep in package.requires if dep.name == "demo"]
+    assert len(demo_dependencies) == 2
+    assert demo_dependencies[0] == demo_dependencies[1]
+    assert {str(dep.marker) for dep in demo_dependencies} == {
+        'sys_platform == "win32"',
+        'sys_platform == "linux"',
+    }
+
+    # directory dependency
+    simple_project = dependencies["simple-project"]
+    assert simple_project.is_directory()
+    assert simple_project.pretty_constraint == "*"
+    simple_project = cast(DirectoryDependency, simple_project)
+    assert simple_project.path == Path("../simple_project")
+    with pytest.raises(AttributeError):
+        simple_project.directory  # type: ignore[attr-defined]
+
+    # url dependency
+    foo = dependencies["foo"]
+    assert foo.is_url()
+    assert foo.pretty_constraint == "*"
+    foo = cast(URLDependency, foo)
+    assert foo.url == "https://example.com/foo.zip"
+    assert foo.directory == "sub"
+
+
 def test_create_poetry_with_packages_and_includes() -> None:
     poetry = Factory().create_poetry(
         fixtures_dir.parent / "masonry" / "builders" / "fixtures" / "with-include"