diff --git a/Lib/test/test_perf_profiler.py b/Lib/test/test_perf_profiler.py
index 13424991639215..e6852c93e69830 100644
--- a/Lib/test/test_perf_profiler.py
+++ b/Lib/test/test_perf_profiler.py
@@ -238,6 +238,24 @@ def test_sys_api_get_status(self):
                 """
         assert_python_ok("-c", code, PYTHON_JIT="0")
 
+    def test_sys_api_perf_jit_backend(self):
+        code = """if 1:
+                import sys
+                sys.activate_stack_trampoline("perf_jit")
+                assert sys.is_stack_trampoline_active() is True
+                sys.deactivate_stack_trampoline()
+                assert sys.is_stack_trampoline_active() is False
+                """
+        assert_python_ok("-c", code, PYTHON_JIT="0")
+
+    def test_sys_api_with_existing_perf_jit_trampoline(self):
+        code = """if 1:
+                import sys
+                sys.activate_stack_trampoline("perf_jit")
+                sys.activate_stack_trampoline("perf_jit")
+                """
+        assert_python_ok("-c", code, PYTHON_JIT="0")
+
 
 def is_unwinding_reliable_with_frame_pointers():
     cflags = sysconfig.get_config_var("PY_CORE_CFLAGS")
diff --git a/Misc/NEWS.d/next/Core_and_Builtins/2025-11-15-01-21-00.gh-issue-141579.aB7cD9.rst b/Misc/NEWS.d/next/Core_and_Builtins/2025-11-15-01-21-00.gh-issue-141579.aB7cD9.rst
new file mode 100644
index 00000000000000..8ab9979c39917b
--- /dev/null
+++ b/Misc/NEWS.d/next/Core_and_Builtins/2025-11-15-01-21-00.gh-issue-141579.aB7cD9.rst
@@ -0,0 +1,2 @@
+Fix :func:`sys.activate_stack_trampoline` to properly support the
+``perf_jit`` backend. Patch by Pablo Galindo.
diff --git a/Python/sysmodule.c b/Python/sysmodule.c
index 59baca26793f6c..f06e639a98f310 100644
--- a/Python/sysmodule.c
+++ b/Python/sysmodule.c
@@ -2378,14 +2378,14 @@ sys_activate_stack_trampoline_impl(PyObject *module, const char *backend)
                 return NULL;
             }
         }
-        else if (strcmp(backend, "perf_jit") == 0) {
-            _PyPerf_Callbacks cur_cb;
-            _PyPerfTrampoline_GetCallbacks(&cur_cb);
-            if (cur_cb.write_state != _Py_perfmap_jit_callbacks.write_state) {
-                if (_PyPerfTrampoline_SetCallbacks(&_Py_perfmap_jit_callbacks) < 0 ) {
-                    PyErr_SetString(PyExc_ValueError, "can't activate perf jit trampoline");
-                    return NULL;
-                }
+    }
+    else if (strcmp(backend, "perf_jit") == 0) {
+        _PyPerf_Callbacks cur_cb;
+        _PyPerfTrampoline_GetCallbacks(&cur_cb);
+        if (cur_cb.write_state != _Py_perfmap_jit_callbacks.write_state) {
+            if (_PyPerfTrampoline_SetCallbacks(&_Py_perfmap_jit_callbacks) < 0 ) {
+                PyErr_SetString(PyExc_ValueError, "can't activate perf jit trampoline");
+                return NULL;
             }
         }
     }