diff --git a/Lib/pdb.py b/Lib/pdb.py
index 8e9502cb9e6bfb..60b713ebaf3d1a 100644
--- a/Lib/pdb.py
+++ b/Lib/pdb.py
@@ -654,7 +654,7 @@ def _show_display(self):
 
     def _get_tb_and_exceptions(self, tb_or_exc):
         """
-        Given a tracecack or an exception, return a tuple of chained exceptions
+        Given a traceback or an exception, return a tuple of chained exceptions
         and current traceback to inspect.
 
         This will deal with selecting the right ``__cause__`` or ``__context__``
@@ -2429,7 +2429,9 @@ def print_stack_trace(self, count=None):
         except KeyboardInterrupt:
             pass
 
-    def print_stack_entry(self, frame_lineno, prompt_prefix=line_prefix):
+    def print_stack_entry(self, frame_lineno, prompt_prefix=None):
+        if prompt_prefix is None:
+            prompt_prefix = line_prefix
         frame, lineno = frame_lineno
         if frame is self.curframe:
             prefix = '> '
diff --git a/Lib/test/test_pdb.py b/Lib/test/test_pdb.py
index 418ea79cdd22c3..34dfc220c7ed73 100644
--- a/Lib/test/test_pdb.py
+++ b/Lib/test/test_pdb.py
@@ -3580,6 +3580,20 @@ def quux():
             ('bœr', 5),
         )
 
+    def test_print_stack_entry_uses_dynamic_line_prefix(self):
+        """Test that pdb.line_prefix binding is dynamic (gh-141781)."""
+        stdout = io.StringIO()
+        p = pdb.Pdb(stdout=stdout)
+
+        # Get the current frame to use for printing
+        frame = sys._getframe()
+
+        with support.swap_attr(pdb, 'line_prefix', 'CUSTOM_PREFIX> '):
+            p.print_stack_entry((frame, frame.f_lineno))
+
+        # Check if the custom prefix appeared in the output
+        self.assertIn('CUSTOM_PREFIX> ', stdout.getvalue())
+
     def test_find_function_found_with_encoding_cookie(self):
         self._assert_find_function(
             """\
diff --git a/Misc/ACKS b/Misc/ACKS
index f5f15f2eb7ea24..ab6b8662d8fc81 100644
--- a/Misc/ACKS
+++ b/Misc/ACKS
@@ -927,6 +927,7 @@ Kristján Valur Jónsson
 Jens B. Jorgensen
 John Jorgensen
 Sijin Joseph
+Paresh Joshi
 Andreas Jung
 Tattoo Mabonzo K.
 Sarah K.
diff --git a/Misc/NEWS.d/next/Library/2025-11-24-06-44-45.gh-issue-141781.MsK27r.rst b/Misc/NEWS.d/next/Library/2025-11-24-06-44-45.gh-issue-141781.MsK27r.rst
new file mode 100644
index 00000000000000..21db97025a3d50
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2025-11-24-06-44-45.gh-issue-141781.MsK27r.rst
@@ -0,0 +1 @@
+Fixed an issue where pdb.line_prefix assignment was ignored if assigned after the module was imported.