diff --git a/Misc/NEWS.d/next/C_API/2025-11-18-04-16-09.gh-issue-140042.S1C7id.rst b/Misc/NEWS.d/next/C_API/2025-11-18-04-16-09.gh-issue-140042.S1C7id.rst
new file mode 100644
index 00000000000000..608e806b431372
--- /dev/null
+++ b/Misc/NEWS.d/next/C_API/2025-11-18-04-16-09.gh-issue-140042.S1C7id.rst
@@ -0,0 +1 @@
+Removed the sqlite3_shutdown call that could cause closing connections for sqlite when used with multiple sub interpreters.
diff --git a/Modules/_sqlite/module.c b/Modules/_sqlite/module.c
index 27e8dab92e0e67..fcc1d69c707ebe 100644
--- a/Modules/_sqlite/module.c
+++ b/Modules/_sqlite/module.c
@@ -745,7 +745,6 @@ module_exec(PyObject *module)
     return 0;
 
 error:
-    sqlite3_shutdown();
     return -1;
 }