diff --git a/spring-web/src/main/java/org/springframework/web/method/support/InvocableHandlerMethod.java b/spring-web/src/main/java/org/springframework/web/method/support/InvocableHandlerMethod.java
index 1ed1a131bf38..4837675beb44 100644
--- a/spring-web/src/main/java/org/springframework/web/method/support/InvocableHandlerMethod.java
+++ b/spring-web/src/main/java/org/springframework/web/method/support/InvocableHandlerMethod.java
@@ -1,5 +1,5 @@
 /*
- * Copyright 2002-2023 the original author or authors.
+ * Copyright 2002-2024 the original author or authors.
  *
  * Licensed under the Apache License, Version 2.0 (the "License");
  * you may not use this file except in compliance with the License.
@@ -56,6 +56,7 @@
  * @author Rossen Stoyanchev
  * @author Juergen Hoeller
  * @author Sebastien Deleuze
+ * @author Yanming Zhou
  * @since 3.1
  */
 public class InvocableHandlerMethod extends HandlerMethod {
@@ -78,6 +79,9 @@ public class InvocableHandlerMethod extends HandlerMethod {
 	@Nullable
 	private MethodValidator methodValidator;
 
+	@Nullable
+	private Class<?>[] validationGroups;
+
 
 	/**
 	 * Create an instance from a {@code HandlerMethod}.
@@ -180,7 +184,10 @@ public Object invokeForRequest(NativeWebRequest request, @Nullable ModelAndViewC
 			logger.trace("Arguments: " + Arrays.toString(args));
 		}
 
-		Class<?>[] groups = getValidationGroups();
+		Class<?>[] groups = this.validationGroups;
+		if (groups == null) {
+			groups = this.validationGroups = this.getValidationGroups();
+		}
 		if (shouldValidateArguments() && this.methodValidator != null) {
 			this.methodValidator.applyArgumentValidation(
 					getBean(), getBridgedMethod(), getMethodParameters(), args, groups);
diff --git a/spring-webflux/src/main/java/org/springframework/web/reactive/result/method/InvocableHandlerMethod.java b/spring-webflux/src/main/java/org/springframework/web/reactive/result/method/InvocableHandlerMethod.java
index 5df721559f1f..3880a0c51d65 100644
--- a/spring-webflux/src/main/java/org/springframework/web/reactive/result/method/InvocableHandlerMethod.java
+++ b/spring-webflux/src/main/java/org/springframework/web/reactive/result/method/InvocableHandlerMethod.java
@@ -1,5 +1,5 @@
 /*
- * Copyright 2002-2023 the original author or authors.
+ * Copyright 2002-2024 the original author or authors.
  *
  * Licensed under the Apache License, Version 2.0 (the "License");
  * you may not use this file except in compliance with the License.
@@ -64,6 +64,7 @@
  * @author Rossen Stoyanchev
  * @author Juergen Hoeller
  * @author Sebastien Deleuze
+ * @author Yanming Zhou
  * @since 5.0
  */
 public class InvocableHandlerMethod extends HandlerMethod {
@@ -87,6 +88,9 @@ public class InvocableHandlerMethod extends HandlerMethod {
 	@Nullable
 	private MethodValidator methodValidator;
 
+	@Nullable
+	private Class<?>[] validationGroups;
+
 
 	/**
 	 * Create an instance from a {@code HandlerMethod}.
@@ -166,7 +170,10 @@ public Mono<HandlerResult> invoke(
 			ServerWebExchange exchange, BindingContext bindingContext, Object... providedArgs) {
 
 		return getMethodArgumentValues(exchange, bindingContext, providedArgs).flatMap(args -> {
-			Class<?>[] groups = getValidationGroups();
+			Class<?>[] groups = this.validationGroups;
+			if (groups == null) {
+				groups = this.validationGroups = this.getValidationGroups();
+			}
 			if (shouldValidateArguments() && this.methodValidator != null) {
 				this.methodValidator.applyArgumentValidation(
 						getBean(), getBridgedMethod(), getMethodParameters(), args, groups);