diff --git a/.gitignore b/.gitignore
index 8b13789..97c2c74 100644
--- a/.gitignore
+++ b/.gitignore
@@ -1 +1,2 @@
-
+**.fai
+.DS_Store
\ No newline at end of file
diff --git a/index.json b/index.json
index 3c352eb..ddd38d1 100644
--- a/index.json
+++ b/index.json
@@ -1,5 +1,5 @@
{
- "github-commit-sha": "37262064a37de3ca1a5498088ba154119d2ecd90",
+ "github-commit-sha": "124367a0243d03d5b3bb57dd9e8c328f9e6759c7",
"primerschemes": {
"artic-measles": {
"400": {
@@ -13,10 +13,10 @@
],
"citations": [],
"collections": [
- "WHOLE-GENOME",
- "ARTIC",
"WASTE-WATER",
- "QUICK-LAB"
+ "QUICK-LAB",
+ "WHOLE-GENOME",
+ "ARTIC"
],
"info_json_url": "https://raw.githubusercontent.com/quick-lab/primerschemes/main/primerschemes/artic-measles/400/v1.0.0/info.json",
"license": "CC BY-SA 4.0",
@@ -30,7 +30,7 @@
"species": [
11234
],
- "status": "draft"
+ "status": "tested"
}
}
},
@@ -375,14 +375,14 @@
"ampliconsize": 600,
"articbedversion": "v3.0",
"authors": [
- "Quick Lab",
"Dr Sheila Lumley",
- "Chris Kent"
+ "Chris Kent",
+ "Quick Lab"
],
"citations": [],
"collections": [
- "QUICK-LAB",
- "WHOLE-GENOME"
+ "WHOLE-GENOME",
+ "QUICK-LAB"
],
"info_json_url": "https://raw.githubusercontent.com/quick-lab/primerschemes/main/primerschemes/hbv/600/v2.0.0/info.json",
"license": "CC BY-SA 4.0",
@@ -397,6 +397,34 @@
10407
],
"status": "tested"
+ },
+ "v2.1.0": {
+ "algorithmversion": "primalscheme3:1.1.4",
+ "ampliconsize": 600,
+ "articbedversion": "v3.0",
+ "authors": [
+ "Chris Kent c.g.kent@bham.ac.uk",
+ "Dr Sheila Lumley sheila.lumley@trinity.ox.ac.uk",
+ "Quick Lab"
+ ],
+ "citations": [],
+ "collections": [
+ "QUICK-LAB",
+ "WHOLE-GENOME"
+ ],
+ "info_json_url": "https://raw.githubusercontent.com/ChrisgKent/primerschemes-fork/main/primerschemes/hbv/600/v2.1.0/info.json",
+ "license": "CC BY-SA 4.0",
+ "primer_bed_md5": "5af354939e06acc830f7f340720a94b1",
+ "primer_bed_url": "https://raw.githubusercontent.com/ChrisgKent/primerschemes-fork/main/primerschemes/hbv/600/v2.1.0/primer.bed",
+ "primerclass": "primerschemes",
+ "reference_fasta_md5": "b0c81b63db38a860016236b68977f38e",
+ "reference_fasta_url": "https://raw.githubusercontent.com/ChrisgKent/primerschemes-fork/main/primerschemes/hbv/600/v2.1.0/reference.fasta",
+ "schemename": "hbv",
+ "schemeversion": "v2.1.0",
+ "species": [
+ 10407
+ ],
+ "status": "draft"
}
}
},
@@ -490,7 +518,7 @@
"species": [
122929
],
- "status": "draft"
+ "status": "tested"
}
}
},
diff --git a/primerschemes/artic-measles/400/v1.0.0/README.md b/primerschemes/artic-measles/400/v1.0.0/README.md
index 8f3ccee..3c7e928 100644
--- a/primerschemes/artic-measles/400/v1.0.0/README.md
+++ b/primerschemes/artic-measles/400/v1.0.0/README.md
@@ -17,7 +17,7 @@ A pan Measles morbillivirus scheme. All full length genomes have been downloaded
"schemename": "artic-measles",
"primer_bed_md5": "b8b903356d4c6bd4dc03af04f3a18222",
"reference_fasta_md5": "2c3dd74742a333dffb50bcfbd158bfbd",
- "status": "draft",
+ "status": "tested",
"citations": [],
"authors": [
"Chris Kent",
@@ -34,10 +34,10 @@ A pan Measles morbillivirus scheme. All full length genomes have been downloaded
"description": "A pan Measles morbillivirus scheme. All full length genomes have been downloaded and phylogenicly downsampled to 0.95 RTL.",
"derivedfrom": null,
"collections": [
- "WHOLE-GENOME",
- "ARTIC",
"WASTE-WATER",
- "QUICK-LAB"
+ "QUICK-LAB",
+ "WHOLE-GENOME",
+ "ARTIC"
]
}
```
diff --git a/primerschemes/artic-measles/400/v1.0.0/info.json b/primerschemes/artic-measles/400/v1.0.0/info.json
index c3afd7a..baca269 100644
--- a/primerschemes/artic-measles/400/v1.0.0/info.json
+++ b/primerschemes/artic-measles/400/v1.0.0/info.json
@@ -4,7 +4,7 @@
"schemename": "artic-measles",
"primer_bed_md5": "b8b903356d4c6bd4dc03af04f3a18222",
"reference_fasta_md5": "2c3dd74742a333dffb50bcfbd158bfbd",
- "status": "draft",
+ "status": "tested",
"citations": [],
"authors": [
"Chris Kent",
@@ -21,9 +21,9 @@
"description": "A pan Measles morbillivirus scheme. All full length genomes have been downloaded and phylogenicly downsampled to 0.95 RTL.",
"derivedfrom": null,
"collections": [
- "WHOLE-GENOME",
- "ARTIC",
"WASTE-WATER",
- "QUICK-LAB"
+ "QUICK-LAB",
+ "WHOLE-GENOME",
+ "ARTIC"
]
}
\ No newline at end of file
diff --git a/primerschemes/hbv/600/v2.0.0/README.md b/primerschemes/hbv/600/v2.0.0/README.md
index 2bf45e8..3c72961 100644
--- a/primerschemes/hbv/600/v2.0.0/README.md
+++ b/primerschemes/hbv/600/v2.0.0/README.md
@@ -2,7 +2,7 @@
## Description
-A 800bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce bias in under represented HBV Clades
+A 600bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce bias in under represented HBV Clades
## Overviews
@@ -20,9 +20,9 @@ A 800bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce b
"status": "tested",
"citations": [],
"authors": [
- "Quick Lab",
"Dr Sheila Lumley",
- "Chris Kent"
+ "Chris Kent",
+ "Quick Lab"
],
"algorithmversion": "primalscheme3:1.1.3",
"species": [
@@ -32,11 +32,11 @@ A 800bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce b
"primerclass": "primerschemes",
"infoschema": "v1.3.0",
"articbedversion": "v3.0",
- "description": "A 800bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce bias in under represented HBV Clades",
+ "description": "A 600bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce bias in under represented HBV Clades",
"derivedfrom": null,
"collections": [
- "QUICK-LAB",
- "WHOLE-GENOME"
+ "WHOLE-GENOME",
+ "QUICK-LAB"
]
}
```
diff --git a/primerschemes/hbv/600/v2.0.0/info.json b/primerschemes/hbv/600/v2.0.0/info.json
index 5074b46..4775ac1 100644
--- a/primerschemes/hbv/600/v2.0.0/info.json
+++ b/primerschemes/hbv/600/v2.0.0/info.json
@@ -7,9 +7,9 @@
"status": "tested",
"citations": [],
"authors": [
- "Quick Lab",
"Dr Sheila Lumley",
- "Chris Kent"
+ "Chris Kent",
+ "Quick Lab"
],
"algorithmversion": "primalscheme3:1.1.3",
"species": [
@@ -19,10 +19,10 @@
"primerclass": "primerschemes",
"infoschema": "v1.3.0",
"articbedversion": "v3.0",
- "description": "A 800bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce bias in under represented HBV Clades",
+ "description": "A 600bp high overlap scheme for HBV. Using phylogenetic downsampling to reduce bias in under represented HBV Clades",
"derivedfrom": null,
"collections": [
- "QUICK-LAB",
- "WHOLE-GENOME"
+ "WHOLE-GENOME",
+ "QUICK-LAB"
]
}
\ No newline at end of file
diff --git a/primerschemes/hbv/600/v2.1.0/README.md b/primerschemes/hbv/600/v2.1.0/README.md
new file mode 100644
index 0000000..f44a358
--- /dev/null
+++ b/primerschemes/hbv/600/v2.1.0/README.md
@@ -0,0 +1,50 @@
+# hbv 600bp v2.1.0
+
+## Description
+
+Contains additional Spike in primers for clades G and H
+
+## Overviews
+
+
+
+## Details
+
+```json
+{
+ "ampliconsize": 600,
+ "schemeversion": "v2.1.0",
+ "schemename": "hbv",
+ "primer_bed_md5": "5af354939e06acc830f7f340720a94b1",
+ "reference_fasta_md5": "b0c81b63db38a860016236b68977f38e",
+ "status": "draft",
+ "citations": [],
+ "authors": [
+ "Chris Kent c.g.kent@bham.ac.uk",
+ "Dr Sheila Lumley sheila.lumley@trinity.ox.ac.uk",
+ "Quick Lab"
+ ],
+ "algorithmversion": "primalscheme3:1.1.4",
+ "species": [
+ 10407
+ ],
+ "license": "CC BY-SA 4.0",
+ "primerclass": "primerschemes",
+ "infoschema": "v1.3.0",
+ "articbedversion": "v3.0",
+ "description": "Contains additional Spike in primers for clades G and H",
+ "derivedfrom": "hbv/600/v2.0.0",
+ "collections": [
+ "QUICK-LAB",
+ "WHOLE-GENOME"
+ ]
+}
+```
+
+
+
+------------------------------------------------------------------------
+
+This work is licensed under a [Creative Commons Attribution-ShareAlike 4.0 International License](http://creativecommons.org/licenses/by-sa/4.0/)
+
+
\ No newline at end of file
diff --git a/primerschemes/hbv/600/v2.1.0/info.json b/primerschemes/hbv/600/v2.1.0/info.json
new file mode 100644
index 0000000..b216f22
--- /dev/null
+++ b/primerschemes/hbv/600/v2.1.0/info.json
@@ -0,0 +1,28 @@
+{
+ "ampliconsize": 600,
+ "schemeversion": "v2.1.0",
+ "schemename": "hbv",
+ "primer_bed_md5": "5af354939e06acc830f7f340720a94b1",
+ "reference_fasta_md5": "b0c81b63db38a860016236b68977f38e",
+ "status": "draft",
+ "citations": [],
+ "authors": [
+ "Chris Kent c.g.kent@bham.ac.uk",
+ "Dr Sheila Lumley sheila.lumley@trinity.ox.ac.uk",
+ "Quick Lab"
+ ],
+ "algorithmversion": "primalscheme3:1.1.4",
+ "species": [
+ 10407
+ ],
+ "license": "CC BY-SA 4.0",
+ "primerclass": "primerschemes",
+ "infoschema": "v1.3.0",
+ "articbedversion": "v3.0",
+ "description": "Contains additional Spike in primers for clades G and H",
+ "derivedfrom": "hbv/600/v2.0.0",
+ "collections": [
+ "QUICK-LAB",
+ "WHOLE-GENOME"
+ ]
+}
\ No newline at end of file
diff --git a/primerschemes/hbv/600/v2.1.0/primer.bed b/primerschemes/hbv/600/v2.1.0/primer.bed
new file mode 100644
index 0000000..aefb12e
--- /dev/null
+++ b/primerschemes/hbv/600/v2.1.0/primer.bed
@@ -0,0 +1,133 @@
+# artic-bed-version v3.0
+X02763 97 123 f3d7635a_0_LEFT_0 1 + AATACTGCCTCTGCCATATCATCAAC
+X02763 98 123 f3d7635a_0_LEFT_1 1 + ACACTGCCTCTTCCATATCGTCAAT
+X02763 97 123 f3d7635a_0_LEFT_2 1 + ACTACTGCCTCTCACATATCGTCAAT
+X02763 97 123 f3d7635a_0_LEFT_3 1 + ACTACTGCCTCTCACTTATCGTCAAT
+X02763 97 123 f3d7635a_0_LEFT_4 1 + ACTATTGCCTCTCACATCTCGTCAAT
+X02763 100 123 f3d7635a_0_LEFT_5 1 + ACTGCCTCACCCATATCGTCAAT
+X02763 100 123 f3d7635a_0_LEFT_6 1 + ACTGCCTCACTCATCTCGTCAAT
+X02763 100 123 f3d7635a_0_LEFT_7 1 + ACTGCCTCTCACATCTCGTCAAT
+X02763 100 123 f3d7635a_0_LEFT_8 1 + ACTGCCTCTGCCATATCGTCAAC
+X02763 95 123 f3d7635a_0_LEFT_9 1 + AGAATACTGCCTCTTCCATATCGTCAAT
+X02763 95 123 f3d7635a_0_LEFT_10 1 + AGAATACTGTCTCAGCCATATCGTCAAT
+X02763 95 123 f3d7635a_0_LEFT_11 1 + AGAATACTGTCTCTGCCATATCGTCAAT
+X02763 95 123 f3d7635a_0_LEFT_12 1 + CAAATATTGCCTCTCACATCTCGTCAAT
+X02763 99 123 f3d7635a_0_LEFT_13 1 + CACTGCCTCTCCCATATCGTCAAT
+X02763 95 123 f3d7635a_0_LEFT_14 1 + CGACTACTGTCTCTCACATATCGTCAAT
+X02763 95 123 f3d7635a_0_LEFT_15 1 + CGACTATTGCCTCTCTCACATCATCAAT
+X02763 98 123 f3d7635a_0_LEFT_16 1 + CTACTGCCTCTCCCATATCGTCAAT
+X02763 98 123 f3d7635a_0_LEFT_17 1 + CTACTGCCTCTCCCTTATCGTCAAT
+X02763 96 123 f3d7635a_0_LEFT_18 1 + GAATATTGCCTCTCACATCTCGTCAAT
+X02763 96 123 f3d7635a_0_LEFT_19 1 + GACTACTGTCTCTCCCATATCGTCAAT
+X02763 102 123 f3d7635a_0_LEFT_20 1 + TGCCTCTCCCACATCGTCAAT
+X02763 737 760 f3d7635a_0_RIGHT_0 1 - CCCCCAATACCACATCATCCACA
+X02763 737 761 f3d7635a_0_RIGHT_1 1 - GCCCCCAAAACCACATCATCCATA
+X02763 737 761 f3d7635a_0_RIGHT_2 1 - GCCCCCAATACCACATCATCCATA
+X02763 737 761 f3d7635a_0_RIGHT_3 1 - GCCCCCAATACCAGATCATCCATA
+X02763 737 761 f3d7635a_0_RIGHT_4 1 - GCCCCCAGTACCACATCATCCATA
+X02763 737 762 f3d7635a_0_RIGHT_5 1 - GGCCCCCAAAACCATATCATCCATA
+X02763 737 762 f3d7635a_0_RIGHT_6 1 - GGCCCCCAATACCAAATCATCCATA
+X02763 737 762 f3d7635a_0_RIGHT_7 1 - GGCCCCCAATACCACATCATCAATA
+X02763 737 762 f3d7635a_0_RIGHT_8 1 - GGCCCCCAATACCATATCATCCATA
+X02763 588 614 f3d7635a_1_LEFT_0 2 + AAATTGCACTTGTATTCCCATCCCAT
+X02763 590 614 f3d7635a_1_LEFT_1 2 + ACTGCACTTGTATTCCCATCCCAT
+X02763 592 614 f3d7635a_1_LEFT_2 2 + TGCACCTGTATTCCCATCCCAT
+X02763 1168 1188 f3d7635a_1_RIGHT_0 2 - AGCAAACACTTGGCACAGGC
+X02763 1168 1191 f3d7635a_1_RIGHT_1 2 - ATCAGCAAACACTTGGCAGAGAC
+X02763 1168 1192 f3d7635a_1_RIGHT_2 2 - CATCAGCAAACACTTGGCAAAGAC
+X02763 1168 1192 f3d7635a_1_RIGHT_3 2 - CGTCAGCAAACACTTGGCAAAGAC
+X02763 1168 1192 f3d7635a_1_RIGHT_4 2 - CGTCAGCAAACACTTGGCATAGAC
+X02763 1168 1193 f3d7635a_1_RIGHT_5 2 - GCATCAGCAAACACTTGGCATAGAC
+X02763 1168 1191 f3d7635a_1_RIGHT_6 2 - GTCAGCAAACACTTGGCAGAGAC
+X02763 1168 1190 f3d7635a_1_RIGHT_7 2 - TCAGCAAACACTTGGCAAAGGC
+X02763 1168 1190 f3d7635a_1_RIGHT_8 2 - TCAGCAAACACTTGGCACAGAC
+X02763 1081 1106 f3d7635a_2_LEFT_0 1 + AACAGGCTTTTACTTTTTCGCCAAC
+X02763 1082 1106 f3d7635a_2_LEFT_1 1 + ACAGGCTTTTACTTTCTCGCCAAC
+X02763 1084 1106 f3d7635a_2_LEFT_2 1 + AGGCTTTCACTTTCTCGCCAAC
+X02763 1083 1106 f3d7635a_2_LEFT_3 1 + CAGGCTTTCACTTTTTCGCCAAC
+X02763 1083 1106 f3d7635a_2_LEFT_4 1 + CAGGCTTTTGTCTTTTCGCCAAC
+X02763 1082 1106 f3d7635a_2_LEFT_5 1 + GCAAGCTTTCACTTTCTCGCCAAC
+X02763 1082 1106 f3d7635a_2_LEFT_6 1 + GCAGGCTTTTACTTTCTCGCCAAC
+X02763 1083 1106 de464cad_2_LEFT_7 1 + CAGGCTTTTGTTTTCTCGCCAAC
+X02763 1692 1720 f3d7635a_2_RIGHT_0 1 - ACACAGTCTTTGATGTATTCTTCGATCC
+X02763 1692 1718 f3d7635a_2_RIGHT_1 1 - ACAGTCTTTGAAGTAAGCCTCAAGGT
+X02763 1692 1718 f3d7635a_2_RIGHT_2 1 - ACAGTCTTTGAAGTATGCCTCAAGGT
+X02763 1692 1716 f3d7635a_2_RIGHT_3 1 - AGTCTTTGAAGTACGCCTCAAGGT
+X02763 1692 1716 f3d7635a_2_RIGHT_4 1 - AGTCTTTGAAGTAGGCCTCAAGGT
+X02763 1692 1719 de464cad_2_RIGHT_1 1 + CACAGTCCTTGAAGTATTTCTCCATCC
+X02763 1692 1720 de464cad_2_RIGHT_2 1 + ACACAGTCTTTGATGTATTCCTCAATCC
+X02763 1692 1718 de464cad_2_RIGHT_3 1 + ACAGTCCTTGAAGTATTTCTCCACCC
+X02763 1640 1666 f3d7635a_3_LEFT_0 2 + CCAACAGTCTTACATAAGCGGACTCT
+X02763 1641 1666 f3d7635a_3_LEFT_1 2 + CCAAGGTCTTACATAAGCGGACTCT
+X02763 1641 1666 f3d7635a_3_LEFT_2 2 + CCAAGGTCTTGCATAAGAGGACTCT
+X02763 1640 1666 f3d7635a_3_LEFT_3 2 + CCCAAGGTCTTACATAAGAGGACTCT
+X02763 1639 1666 f3d7635a_3_LEFT_4 2 + GCCAACAGTCTTACATAAGAGGACTCT
+X02763 1639 1666 f3d7635a_3_LEFT_5 2 + GCCCAAGGTCTTACATAAAAGGACTCT
+X02763 1639 1666 f3d7635a_3_LEFT_6 2 + GCCCAAGGTCTTACATAAGAGGACTAT
+X02763 1639 1666 f3d7635a_3_LEFT_7 2 + GCCCAAGGTCTTATATAAGAGGACTCT
+X02763 1639 1666 f3d7635a_3_LEFT_8 2 + GCCTAAGGTCTTACATAAGAGGACTCT
+X02763 1638 1666 de464cad_3_LEFT_9 2 + TGCCAAAGCAGTTATATAAGAGGACTCT
+X02763 1639 1666 de464cad_3_LEFT_10 2 + GCCAAGGCAGTTATATAAGAGGACTCT
+X02763 2299 2325 f3d7635a_3_RIGHT_0 2 - ATTGATAAGATAGGGGCATTTGGTGG
+X02763 2299 2326 f3d7635a_3_RIGHT_1 2 - CGTTGATAAGATAGGGGCATTTTGTGG
+X02763 2299 2323 f3d7635a_3_RIGHT_2 2 - GGATAGGATAGGGGCATTTGGTGG
+X02763 2299 2325 f3d7635a_3_RIGHT_3 2 - GTTGATAAGATAGGGGCATTTGCTGG
+X02763 2299 2325 f3d7635a_3_RIGHT_4 2 - GTTGATAAGATAGGGGCATTTGGAGG
+X02763 2299 2325 f3d7635a_3_RIGHT_5 2 - GTTGATAAGATAGGGGCATTTGGTGG
+X02763 2299 2323 f3d7635a_3_RIGHT_6 2 - TGATAGGATAGGGGCATTTGGTGG
+X02763 2299 2324 f3d7635a_3_RIGHT_7 2 - TGGATAAGATAGGGGCATTTGGTGG
+X02763 2299 2326 f3d7635a_3_RIGHT_8 2 - TGTTGATAAGATAGGGGCATTAGGTGG
+X02763 2299 2326 f3d7635a_3_RIGHT_9 2 - TGTTGATAAGATAGGGGCATTTGATGG
+X02763 2299 2326 f3d7635a_3_RIGHT_10 2 - TGTTGATAAGATAGGGGCATTTGTTGG
+X02763 2299 2326 f3d7635a_3_RIGHT_11 2 - TGTTGATAAGATAGGGGCATTTTGTGG
+X02763 2204 2232 f3d7635a_4_LEFT_0 1 + GGTTCCACATTTCCTGTCTTACTTTTGG
+X02763 2204 2232 f3d7635a_4_LEFT_1 1 + GGTTTCACATCTCCTGTCTTACTTTTGG
+X02763 2204 2232 f3d7635a_4_LEFT_2 1 + GGTTTCACATTTCCTGTCTTACCTTTGG
+X02763 2204 2232 f3d7635a_4_LEFT_3 1 + GGTTTCACATTTCCTGTCTTACTTTCGG
+X02763 2204 2232 f3d7635a_4_LEFT_4 1 + GGTTTCACATTTCCTGTCTTACTTTTGG
+X02763 2204 2232 f3d7635a_4_LEFT_5 1 + GGTTTCACATTTCTTGTCTCACTTTTGG
+X02763 2202 2232 f3d7635a_4_LEFT_6 1 + GTGGTTTCATATATCTTGCCTTACTTTTGG
+X02763 2202 2232 f3d7635a_4_LEFT_7 1 + GTGGTTTCATATTTCCTGTCTTACTTTTGG
+X02763 2205 2232 f3d7635a_4_LEFT_8 1 + GTTTCACACTTCTTGTCTCACTTTTGG
+X02763 2205 2232 f3d7635a_4_LEFT_9 1 + GTTTCACATTTCCTGCCTTACTTTTGG
+X02763 2205 2232 f3d7635a_4_LEFT_10 1 + GTTTCACATTTCCTGTCTCACTTTTGG
+X02763 2205 2232 f3d7635a_4_LEFT_11 1 + GTTTCACGTTTCTTGTCTCACTTTTGG
+X02763 2203 2232 f3d7635a_4_LEFT_12 1 + TGGTTTCACATTTCTTGTCTTACTTTTGG
+X02763 2203 2232 f3d7635a_4_LEFT_13 1 + TGGTTTCATATTTCTTGCCTTACTTTTGG
+X02763 2204 2232 de464cad_4_LEFT_14 1 + GGTTTCATATTTCCTGCCTTACATTTGG
+X02763 2205 2232 de464cad_4_LEFT_15 1 + GTTTCACATTTCCTGCCTTACATTTGG
+X02763 2834 2855 f3d7635a_4_RIGHT_0 1 - ATGCCGTAGCTCTTGTTCCCA
+X02763 2834 2857 f3d7635a_4_RIGHT_1 1 - CCATGCTGTAGCTCTTGTTCCCA
+X02763 2834 2857 f3d7635a_4_RIGHT_2 1 - CCATGCTGTAGCTTTTGTTCCCA
+X02763 2834 2858 f3d7635a_4_RIGHT_3 1 - CCCATGATGTAGCTCTTGTTCCCA
+X02763 2834 2858 f3d7635a_4_RIGHT_4 1 - CCCATGCTGTAGATCTTGTTCCCA
+X02763 2834 2858 f3d7635a_4_RIGHT_5 1 - CCCATGCTGTATCTCTTGTTCCCA
+X02763 2834 2858 f3d7635a_4_RIGHT_6 1 - GGTTTGCTGTAGCTCTTGTTCCCA
+X02763 2768 2794 f3d7635a_5_LEFT_0 2 + AAGGCGGGCATTCTATATAAGAGAGA
+X02763 2769 2794 f3d7635a_5_LEFT_1 2 + AGGCGGGCATCTTATATAAAAGGGA
+X02763 2769 2794 f3d7635a_5_LEFT_2 2 + AGGCTGGGATCCTATATAAGAGGGA
+X02763 2764 2794 f3d7635a_5_LEFT_3 2 + ATGGAAGGCGGGTATATTATATAAGAGAGA
+X02763 2760 2794 f3d7635a_5_LEFT_4 2 + CTTTATGGAAAGCAGGAATTCTATATAAGAGAGA
+X02763 2767 2794 f3d7635a_5_LEFT_5 2 + GAAGGCTGGGATTCTATATAAGAGGGA
+X02763 2766 2794 f3d7635a_5_LEFT_6 2 + GGAAGGCGGGAATCTTATATAAGAGAGA
+X02763 2766 2794 f3d7635a_5_LEFT_7 2 + GGAAGGCGGGCATCTTATATAAAAGAGA
+X02763 2766 2794 f3d7635a_5_LEFT_8 2 + GGAAGGCGGGGATCTTATATAAAAGAGA
+X02763 2766 2794 f3d7635a_5_LEFT_9 2 + GGAAGGCGGGTATCTTATATAAGAGAGA
+X02763 2766 2794 f3d7635a_5_LEFT_10 2 + GGAAGGCGGGTGTATTATATAAGAGAGA
+X02763 2766 2794 f3d7635a_5_LEFT_11 2 + GGAAGGCTGGCATTCTATATAAGAGAGA
+X02763 2766 2794 f3d7635a_5_LEFT_12 2 + GGAAGGCTGGTATTCTATATAAGAGGGA
+X02763 2764 2794 f3d7635a_5_LEFT_13 2 + GTGGAAGGCTGGTATTCTATATAAGAGAGA
+X02763 2765 2794 f3d7635a_5_LEFT_14 2 + TGGAAAGCTGGCATTCTATATAAGAGAGA
+X02763 2765 2794 f3d7635a_5_LEFT_15 2 + TGGAAGGCGGGAATTTTATATAAGAGAGA
+X02763 2765 2794 f3d7635a_5_LEFT_16 2 + TGGAAGGCGGGTATCTTATATAAAAGAGA
+X02763 2765 2794 f3d7635a_5_LEFT_17 2 + TGGAAGGCGGGTATTTTATATAAGAGAGA
+X02763 2765 2794 f3d7635a_5_LEFT_18 2 + TGGAAGGCTGGCATTCTATATAAAAGAGA
+X02763 2765 2794 f3d7635a_5_LEFT_19 2 + TGGAAGGCTGGCATTTTATATAAGAGAGA
+X02763 2763 2794 f3d7635a_5_LEFT_20 2 + TGTGGAAGGCTGGTATTTTATATAAGAGAGA
+X02763 2763 2794 f3d7635a_5_LEFT_21 2 + TTTGGAAGGCGGGTATTTTATATAAAAGAGA
+X02763 2766 2794 de464cad_5_LEFT_22 2 + GGAAGGCGGGTATTCTATATAAGAGAGA
+X02763 2765 2794 de464cad_5_LEFT_23 2 + TGGAAGGCAGGAATTCTATATAAGAGAGA
+X02763 225 249 f3d7635a_5_RIGHT_0 2 - AGACTCTGCGGTATTGTGAGGATT
+X02763 225 254 f3d7635a_5_RIGHT_1 2 - AGTCTAGACTCTTTGGTATTGTGAGGATT
+X02763 225 253 f3d7635a_5_RIGHT_2 2 - GTCTAGACTCTGTGGTATTGTGAGGATT
+X02763 225 253 f3d7635a_5_RIGHT_3 2 - GTCTAGACTCTTCGGTATTGTGAGGATT
\ No newline at end of file
diff --git a/primerschemes/hbv/600/v2.1.0/reference.fasta b/primerschemes/hbv/600/v2.1.0/reference.fasta
new file mode 100644
index 0000000..1da3881
--- /dev/null
+++ b/primerschemes/hbv/600/v2.1.0/reference.fasta
@@ -0,0 +1,55 @@
+>X02763
+TTCCACTGCCTTCCACCAAACTCTGCAGGATCCCAGAGTCAGGGGTCTGTATCTTCCTGC
+TGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTC
+AATCTCCGCGAGGACTGGGGACCCTGTGACGAACATGGAGAACATCACATCAGGATTCCT
+AGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAGAATCCTCACAATACC
+GCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCTCCCGTGTGTCT
+TGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAATTTG
+TCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCT
+ATGCCTCATCTTCTTATTGGTTCTTCTGGATTATCAAGGTATGTTGCCCGTTTGTCCTCT
+AATTCCAGGATCAACAACAACCAGTACGGGACCATGCAAAACCTGCACGACTCCTGCTCA
+AGGCAACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGCACCTG
+TATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCG
+TTTCTCTTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC
+TGTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGT
+GAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCT
+AACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCATGGGCTACATAATTGGAAGTTGG
+GGAACTTTGCCACAGGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCT
+GTTAACAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCT
+GCTCCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCT
+AAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTACATGAAC
+CTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCC
+ACTGGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTG
+CCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTGGAGCAAAG
+CTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGTTTCCATGGCTG
+CTAGGCTGTACTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCG
+CTGAATCCCGCGGACGACCCCTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGT
+CTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCCGTCTGTGCCT
+TCTCATCTGCCGGTCCGTGTGCACTTCGCTTCACCTCTGCACGTTGCATGGAGACCACCG
+TGAACGCCCATCAGATCCTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCCCAGCAA
+TGTCAACGACCGACCTTGAGGCCTACTTCAAAGACTGTGTGTTTAAGGACTGGGAGGAGC
+TGGGGGAGGAGATTAGGTTAAAGGTCTTTGTATTAGGAGGCTGTAGGCACAAATTGGTCT
+GCGCACCAGCACCATGCAACTTTTTCACCTCTGCCTAATCATCTCTTGTACATGTCCCAC
+TGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTATAA
+AGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGT
+CAGAGATCTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGCA
+TTGCTCACCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGAC
+TCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGCATCTAGGGATCTTGTAGTAAA
+TTATGTTAATACTAACGTGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTG
+CCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCG
+CACTCCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC
+TGTTGTTAGACGACGGGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAG
+ACGCAGATCTCCATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTA
+TTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAGTACCTATCT
+TTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAGAGGACATTATTA
+ATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAA
+TTATGCCTGCTAGATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTA
+AACCTTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATTTACATA
+CTCTTTGGAAGGCTGGTATTCTATATAAGCGGGAAACCACACGTAGCGCATCATTTTGCG
+GGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCATCAAAACCTCGC
+AAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCATCAG
+TTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCGTCAAG
+GACGACTGGCCAGCAGCCAACCAAGTAGGAGTGGGAGCATTCGGGCCAAGGCTCACCCCT
+CCACACGGCGGTATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTGTCA
+ACAATTCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCAGCCTACTCCCATCTCT
+CCACCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAA
\ No newline at end of file
diff --git a/primerschemes/hbv/600/v2.1.0/work/X02763.html b/primerschemes/hbv/600/v2.1.0/work/X02763.html
new file mode 100644
index 0000000..7bb9319
--- /dev/null
+++ b/primerschemes/hbv/600/v2.1.0/work/X02763.html
@@ -0,0 +1,14 @@
+
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+
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