Automated 16S sequence extraction using Barrnap output gff files
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16S rRNA sequence extraction from Barrnap outputs


This is a simple automated Shell script that uses the Barrnap ribosomal RNA predictor ( output gff files with 16S gene positions as input to extract single 16S rRNA sequence from original genome FASTA sequence files.


  • Where GFF is the outputs from Barrnap and FASTA is the original FASTA sequence.
  • This script will generate the final sequence file named FASTA-16S.fna, this should contain only single 16S sequence predicted by Barrnap - only the first 16S rRNA sequence predicted will be extracted using this script.