Neural Systems & Behavior Course at the Marine Biological Laboratory
July 7, 2014 – July 19, 2014
Rayna M. Harris Hans A. Hofmann
This laboratory manual was created in the spring of 2013 for use in the Integrative Molecular Neuroethology Module of the Neural Systems & Behavior Course at the Marine Biological Laboratory in Woods Hole, MA. The objective was to teach quantitative real time PCR and immunohistochemistry techniques in one week and then have students conduct independent research projectes in the second week.
Instructor Information
Module Objective
Chapter 1: Gene Identification
Chapter 2: Tissue Processing for Gene Expression Profiling
Chapter 3: RNA Isolation
Chapter 4: Nucleic Acid Quantification
Chapter 5: Reverse Transcription
Chapter 6: Quantitative Real-time PCR (qPCR)
Chapter 7: Immunohistochemistry
Chapter 8: Other Molecular Protocols
Chapter 9: The Laboratory Notebook
Laboratory Notebook Pages
Appendix: Hyperlinks for QR codes
Acknowledgements
This manual has been published on Zenodo.
Rayna M Harris (2016). 2014_NSBLabManual: 2014 Integrative Molecular Neuroethology Module Laboratory Manual. Zenodo. 10.5281/zenodo.60725