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function outnm = nii_nii2objMesh (fnm, thresh, clusterVox, isSmooth, reduce, outnm, floodfill) | ||
%convert NIfTI image to mesh, all arguments are optional | ||
% fnm : nifti image to meshify | ||
% thresh : air/surface threshold, e.g. 2=voxels darker than 2 are air | ||
% n.b. for Altases, set thresh=0: one image will be created per region | ||
% clusterVox : only clusters of with more voxels than this value survive | ||
% isSmooth : if true, input is blurred, otherwise raw data | ||
% reduce : reduction ratio, e.g. 0.2 will decimate 80% of triangles | ||
% outnm : name for output image, e.g. "mesh.obj" | ||
% floodfill : if true, remove bubbles inside objects (e.g. ventricles embedded below cortex) | ||
%Examples | ||
% nii_nii2objMesh %use GUI | ||
% nii_nii2objMesh('motor.nii', 3, 30, false, 0.3); | ||
% nii_nii2objMesh('MNI152_T1_1mm_brain.nii.gz', 5500, 1000000, true, 0.1); | ||
% nii_nii2objMesh('HarvardOxford-cort-maxprob-thr0-1mm.nii.gz', 0, 1, true, 0.1); %convert smoothed atlas | ||
% nii_nii2objMesh('natbrainlab.nii.gz', 0, 1, false, 0.1); %convert unsmoothed atlas | ||
if ~exist('fnm','var') %no files specified | ||
[files,pth] = uigetfile({'*.gz;*.nii;*.hdr;';'*.*'},'Choose images to merge', 'MultiSelect', 'off'); | ||
fnm = strcat(pth,char(files)); | ||
end | ||
%load image | ||
[hdr,img, fnm] = loadNiiSub(fnm); | ||
img(isnan(img)) = 0; | ||
%set preferences | ||
if ~exist('outnm','var') | ||
[pth nm] = spm_fileparts(fnm); | ||
outnm = fullfile(pth, [nm '.obj']); | ||
end; | ||
if ~exist('thresh','var') thresh = (0.5 * max(img(:))-min(img(:))) + min(img(:)); end; | ||
if ~exist('clusterVox','var') clusterVox = 32; end; | ||
if ~exist('isSmooth','var') isSmooth = false; end; | ||
if ~exist('floodfill','var') floodfill = false; end; | ||
fprintf('Image intensity range %g..%g\n', min(img(:)), max(img(:))); | ||
if ~exist('reduce','var') %provide user interface if prefences not specified | ||
prompt = {'Enter threshold (2=only voxels brighter than 2, 0=image is atlas)','Enter minimum cluster size (voxels)','Smooth (blur) input(1=yes, 0=no)','Reduction ratio (0.5=remove 50% of vertices)'}; | ||
dlg_title = 'Values for thresholding'; | ||
num_lines = 1; | ||
def = {num2str(thresh),num2str(clusterVox), num2str(isSmooth), '0.3'}; | ||
answer = inputdlg(prompt,dlg_title,num_lines,def); | ||
thresh = str2double(answer{1}); | ||
clusterVox = str2double(answer{2}); | ||
isSmooth = str2double(answer{3}); | ||
reduce = str2double(answer{4}); | ||
end; | ||
if thresh == 0 | ||
if clusterVox > 1, fprintf('nb: cluster size ignored for Atlases'); end; | ||
if floodfill, fprintf('nb: floodfill ignored for Atlases'); end; | ||
saveAtlasSub(hdr, img, isSmooth, reduce, outnm); | ||
return; | ||
end | ||
if isSmooth | ||
presmooth = img+0; %+0 forces new matrix | ||
spm_smooth(presmooth,img,1,0); %smooth data | ||
end | ||
isInvert = thresh < 0; | ||
if isInvert | ||
img = -img; | ||
thresh = -thresh; | ||
end | ||
[img, nVoxPost] = clusterSub(hdr, img, thresh, clusterVox, isInvert); | ||
if nVoxPost < 1, fprintf('No voxels exceed threshold\n'); return; end; | ||
if floodfill | ||
img = floodSub(img, thresh, 2); %optional: fill bubbles inside | ||
end; | ||
img2meshSub(hdr, img, outnm, reduce, thresh); | ||
%end nii_nii2obj() | ||
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||
function saveAtlasSub(hdr, img, isSmooth, reduce, outnm) | ||
if ~(spm_type(hdr.dt,'intt')) %integer output | ||
error('Threshold=0 suggests Atlas, but image is not saved as discrete integers.'); | ||
end; | ||
mn = min(img(:))+1; | ||
mx = max(img(:)); | ||
if (mn >= mx), error('No variability in input image'); end; | ||
if isSmooth, fprintf('n.b. each region will be smoothed'); end; | ||
[pth nm] = spm_fileparts(outnm); | ||
for i = mn : mx | ||
outnm = fullfile(pth, [nm '_' num2str(i) '.obj']); | ||
bw = (img == i)+0; %black and white image, +0 converts logical->double | ||
if isSmooth | ||
presmooth = bw+0; %+0 forces new matrix | ||
spm_smooth(presmooth,bw,1,0); %smooth data | ||
end | ||
vx = sum(bw(:) >= 0.5); | ||
if vx > 0 | ||
fprintf('Region %d has %d voxels\n', i, vx); | ||
img2meshSub(hdr, bw, outnm, reduce, 0.5); | ||
end; | ||
end | ||
%end saveAtlasSub() | ||
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||
function img2meshSub(hdr, img, outnm, reduce, thresh) | ||
if (max(img(:))< thresh) || (min(img(:)) > thresh) return; end; | ||
FV = isosurface(img,thresh); | ||
FV = reducepatch(FV,reduce); | ||
FV.vertices = FV.vertices(:,[2,1,3]); %isosurface swaps X/Y | ||
vx = [ FV.vertices ones(size(FV.vertices,1),1)]; | ||
vx = mtimes(hdr.mat,vx')'; | ||
FV.vertices = vx(:,1:3); | ||
FV.faces = fliplr(FV.faces); %reverse winding | ||
writeObjSub(FV.vertices,FV.faces, outnm); | ||
%end img2meshSub() | ||
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function [img, nVoxPost] = clusterSub(hdr, img, thresh, clusterVox, isInvert) | ||
bw=img; | ||
bw = (bw >= abs(thresh)) * 1.0; | ||
nVox = sum(bw(:)); | ||
nVoxPost = nVox; | ||
if (clusterVox < 2) || (nVox < 1), return; end; | ||
[bw,nCluster] = spm_bwlabel(bw,18); | ||
nClusterPost = nCluster; | ||
minClusterVox = nCluster; | ||
maxClusterVox = 0; | ||
for i = 1:nCluster | ||
vox = sum(bw(:)== i); | ||
%fprintf('Cluster %d has %d voxels\n', i,vox); | ||
minClusterVox = min(minClusterVox,vox); | ||
maxClusterVox = max(maxClusterVox,vox); | ||
if vox < clusterVox | ||
nVoxPost = nVoxPost - vox; | ||
nClusterPost = nClusterPost - 1; | ||
bw(bw == i) = 0; | ||
end | ||
end | ||
%any voxels that do not survive threshold need to be set just below threshold | ||
bw = (bw < 1); %mask now inverted and logical: TRUEs are voxels that can be reduced | ||
maxsubthresh = thresh - (2 * eps);%max(img(img(:) < thresh)); | ||
mask = (img >= thresh) .* bw; %mask voxels that are over thresh undersized | ||
img(mask == 1) = maxsubthresh; | ||
if isInvert, thresh = - thresh; end; | ||
if minClusterVox ~= maxClusterVox | ||
fprintf('Cluster sizes range from %d to %d voxels\n',minClusterVox, maxClusterVox); | ||
end | ||
outmm3=prod(abs(hdr.mat(1:3, 1:3)*[1;1;1])); | ||
fprintf('%d voxels (%d clusters) exceed %g, of which %d (%d) are in clusters larger than %d voxels (%gmm^3)\n', ... | ||
nVox, nCluster, thresh, nVoxPost, nClusterPost, clusterVox, clusterVox * outmm3); | ||
%end clusterSub() | ||
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function [hdr,img, fnm] = loadNiiSub(fnm) | ||
isGz = false; | ||
if ~exist('spm_fileparts','file'), fprintf('Please install SPM12 or later'); end; | ||
[pth,nam,ext] = spm_fileparts(fnm); | ||
if strcmpi(ext,'.gz') %.nii.gz | ||
fnm = char(gunzip(fnm)); | ||
isGz = true; | ||
elseif strcmpi(ext,'.voi') %.voi -> | ||
onam = char(gunzip(fnm)); | ||
fnm = fullfile(pth, [nam '.nii']); | ||
movefile(onam,fnm); | ||
isGz = true; | ||
end; | ||
hdr = spm_vol(fnm); | ||
img = spm_read_vols(hdr); | ||
if isGz, delete(fnm); end; | ||
%end loadNiiSub() | ||
function writeObjSub(vertex,face,filename) | ||
% --- save Face/Vertex data as WaveFront Object format file | ||
%inputs: | ||
% vertex: vertices matrix where cols are xyz and each row a vertix | ||
% face: face matrix where cols are xyz and each row is face | ||
% fileName: the Wavefront Object file to create | ||
%notes | ||
% https://en.wikipedia.org/wiki/Wavefront_.obj_file | ||
[nF nFd] =size(face); | ||
[nV nVd] =size(vertex); | ||
if (nF <1) || (nV <3 || (nFd ~=3) || (nVd ~=3)), warning('Problem with writeObj'); return; end; %#ok<WNTAG> | ||
fid = fopen(filename, 'wt'); | ||
fprintf(fid, '# WaveFront Object format image created with MRIcroS\n'); | ||
fprintf(fid, 'v %.12g %.12g %.12g\n', vertex'); | ||
fprintf(fid, 'f %d %d %d\n', (face)'); | ||
fclose(fid); | ||
%end writeObjSub() | ||
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function img = floodSub(img, thresh, smoothVox) | ||
%similar to http://www.mathworks.com/matlabcentral/fileexchange/12184-floodfill3d | ||
imgBin = (img < thresh); | ||
imgBin = double(imgBin); % In case a logical matrix comes in. | ||
imgBin(1,:,:) = NaN; %pad LEFT | ||
imgBin(end,:) = NaN; %pad RIGHT | ||
imgBin(:,1,:) = NaN; %pad ANTERIOR | ||
imgBin(:,end,:) = NaN; %pad POSTERIOR | ||
imgBin(:,:,1) = NaN; %pad INFERIOR | ||
imgBin(:,:,end) = NaN; %pad SUPERIOR | ||
imgBin = floodFill3DSub(imgBin, [2,2,2]); | ||
%fill bubbles | ||
mx = max(img(:)); | ||
img(isfinite(imgBin))= mx; | ||
%next: optional - blur to feather edges - only useful if marching cubes uses subvoxel smoothing | ||
maskIn = img + 0.0; | ||
if exist('smoothVox', 'var') | ||
spm_smooth(maskIn, img, smoothVox); %feather the edges a lot: weak blur | ||
end | ||
%end floodSub() | ||
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||
%Copyrights for Matlab Central Code | ||
% stlwriteSub Copyright (c) 2015, Sven Holcombe | ||
% floodFill3DSub Copyright (c) 2006, F Dinath | ||
% | ||
% All rights reserved. | ||
% | ||
% Redistribution and use in source and binary forms, with or without | ||
% modification, are permitted provided that the following conditions are | ||
% met: | ||
% | ||
% * Redistributions of source code must retain the above copyright | ||
% notice, this list of conditions and the following disclaimer. | ||
% * Redistributions in binary form must reproduce the above copyright | ||
% notice, this list of conditions and the following disclaimer in | ||
% the documentation and/or other materials provided with the distribution | ||
% | ||
% THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" | ||
% AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE | ||
% IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE | ||
% ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE | ||
% LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR | ||
% CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF | ||
% SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS | ||
% INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN | ||
% CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) | ||
% ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE | ||
% POSSIBILITY OF SUCH DAMAGE. | ||
|
||
function [A] = floodFill3DSub(A, point) | ||
% http://www.mathworks.com/matlabcentral/fileexchange/12184-floodfill3d | ||
% [B] = FloodFill3D(A, slice); | ||
% This program flood fills a 6-connected 3D region. The input matrix MUST | ||
% be a binary image. The user will select a seed (point) in the matrix to | ||
% initiate the flood fill. You must specify the matrix slice in which you | ||
% wish to place the seed. | ||
% | ||
% A = binary matrix | ||
% slice = a chosen slice in the matrix where you wish to place the seed. | ||
% | ||
% Enjoy, | ||
% F. Dinath | ||
if A(point(1), point(2), point(3)); | ||
A(point(1), point(2), point(3)) = NaN; | ||
a{1} = sub2ind(size(A), point(1), point(2), point(3)); | ||
i = 1; | ||
while 1 | ||
i = i+1; | ||
a{i} = []; %#ok<AGROW> | ||
[x, y, z] = ind2sub(size(A), a{i-1}); | ||
ob = nonzeros((A(sub2ind(size(A), x, y, z-1)) == 1).*sub2ind(size(A), x, y, z-1)); | ||
A(ob) = NaN; | ||
a{i} = [a{i} ob']; %#ok<AGROW> | ||
ob = nonzeros((A(sub2ind(size(A), x, y, z+1)) == 1).*sub2ind(size(A), x, y, z+1)); | ||
A(ob) = NaN; | ||
a{i} = [a{i} ob']; %#ok<AGROW> | ||
ob = nonzeros((A(sub2ind(size(A), x-1, y, z)) == 1).*sub2ind(size(A), x-1, y, z)); | ||
A(ob) = NaN; | ||
a{i} = [a{i} ob']; %#ok<AGROW> | ||
ob = nonzeros((A(sub2ind(size(A), x+1, y, z)) == 1).*sub2ind(size(A), x+1, y, z)); | ||
A(ob) = NaN; | ||
a{i} = [a{i} ob']; %#ok<AGROW> | ||
ob = nonzeros((A(sub2ind(size(A), x, y-1, z)) == 1).*sub2ind(size(A), x, y-1, z)); | ||
A(ob) = NaN; | ||
a{i} = [a{i} ob']; %#ok<AGROW> | ||
ob = nonzeros((A(sub2ind(size(A), x, y+1, z)) == 1).*sub2ind(size(A), x, y+1, z)); | ||
A(ob) = NaN; | ||
a{i} = [a{i} ob']; %#ok<AGROW> | ||
if isempty(a{i}); | ||
break; | ||
end | ||
end | ||
end | ||
%end floodFill3DSub() |
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